2. Neuronal Signaling
  3. Cholinesterase (ChE)
  4. SD-6

SD-6 is an orally active inhibitor of hAChE and hBChE with IC50 values of 0.907 µM and 1.579 µM, respectively. SD-6 has excellent blood-brain barrier (BBB) permeability and no neurotoxicity, which can be used for research on Alzheimer's disease.

For research use only. We do not sell to patients.

SD-6 Chemical Structure

SD-6 Chemical Structure

CAS No. : 744206-31-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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10 mg In-stock
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Based on 1 publication(s) in Google Scholar

SD-6 Related Antibodies

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Description

SD-6 is an orally active inhibitor of hAChE and hBChE with IC50 values of 0.907 µM and 1.579 µM, respectively. SD-6 has excellent blood-brain barrier (BBB) permeability and no neurotoxicity, which can be used for research on Alzheimer's disease[1].

IC50 & Target[1]

hAChE

0.907 μM (IC50)

hBCHE

1.579 μM (IC50)

In Vitro

SD-6 (5-80 µM; 24 h) has neuroprotective effects in SH-SY5Y cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SD-6 Related Antibodies

Cell Viability Assay[1]

Cell Line: SH-SY5Y cells.
Concentration: 5, 10, 20, 40 or 80 µM.
Incubation Time: 24 h.
Result: Exhibited toxicity to differentiated SH-SY5Y cell lines and has a protective effect on undifferentiated SH-SY5Y cell lines.
In Vivo

SD-6 (100, 300 and 500 mg/kg; p.o.; single dose) has no toxic effect on nonpregnant female Wistar rats[1].
SD-6 (2.5, 5 and 10 mg/kg; p.o.; 3 times a day for 7 days) improves cognitive and memory deficits induced by Scopolamine (HY-N0296) in male Wistar rats[1].
SD-6 (2.5, 5 and 10 mg/kg; p.o.; single dose) significantly improves the abnormalities induced by Scopolamine in rats and promotes the normalization of biochemical indicators[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 10–11 weeks nonpregnant female Wistar rats (220–280 g)[1].
Dosage: 100, 300 and 500 mg/kg.
Administration: Oral gavage; single dose.
Result: Showed safety and tolerability.
Animal Model: 10–11 weeks male Wistar rats (220–280 g)[1].
Dosage: 2.5、5、10 mg/kg.
Administration: Oral gavage; 3 times a day for 7 days or single dose.
Result: Had the function of repairing cognitive and memory deficits.
Reduced the level of AChE and malonaldehyde (MDA), increased the level of acetylcholine, superoxide dismutase (SOD), glutathione (GSH) and catalase.
Molecular Weight

366.48

Formula

C20H22N4OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

S=C1OC(C2=CC=CC=C2)=NN1CN3CCN(CC3)CC4=CC=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (68.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7287 mL 13.6433 mL 27.2866 mL
5 mM 0.5457 mL 2.7287 mL 5.4573 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7287 mL 13.6433 mL 27.2866 mL 68.2165 mL
5 mM 0.5457 mL 2.7287 mL 5.4573 mL 13.6433 mL
10 mM 0.2729 mL 1.3643 mL 2.7287 mL 6.8217 mL
15 mM 0.1819 mL 0.9096 mL 1.8191 mL 4.5478 mL
20 mM 0.1364 mL 0.6822 mL 1.3643 mL 3.4108 mL
25 mM 0.1091 mL 0.5457 mL 1.0915 mL 2.7287 mL
30 mM 0.0910 mL 0.4548 mL 0.9096 mL 2.2739 mL
40 mM 0.0682 mL 0.3411 mL 0.6822 mL 1.7054 mL
50 mM 0.0546 mL 0.2729 mL 0.5457 mL 1.3643 mL
60 mM 0.0455 mL 0.2274 mL 0.4548 mL 1.1369 mL
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SD-6 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SD-6744206-31-3SD6SD 6Cholinesterase (ChE)Wistar ratsScopolamineSH-SY5Y cellsInhibitorinhibitorinhibit

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