2. Apoptosis
  3. Apoptosis
  4. SEC inhibitor KL-1

SEC inhibitor KL-1  (Synonyms: KL-1)

Cat. No.: HY-122720 Purity: 99.38%
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SEC inhibitor KL-1 (KL-1) is a peptide-like lead compound that is an effective selective SEC inhibitor. SEC inhibitor KL-1 promotes apoptosis and has anti-tumor activity. SEC inhibitor KL-1 doses dependently inhibits the AFF4-CCNT1 interaction, with a Ki value of 3.48 μM.

For research use only. We do not sell to patients.

SEC inhibitor KL-1 Chemical Structure

SEC inhibitor KL-1 Chemical Structure

CAS No. : 900308-84-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 187 In-stock
Solution
10 mM * 1 mL in DMSO USD 187 In-stock
Solid
5 mg USD 170 In-stock
10 mg USD 270 In-stock
50 mg USD 690 In-stock
100 mg USD 980 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

SEC inhibitor KL-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE SEC inhibitor KL-1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SEC inhibitor KL-1 (KL-1) is a peptide-like lead compound that is an effective selective SEC inhibitor. SEC inhibitor KL-1 promotes apoptosis and has anti-tumor activity. SEC inhibitor KL-1 doses dependently inhibits the AFF4-CCNT1 interaction, with a Ki value of 3.48 μM[1][2].

IC50 & Target

SEC[2]
In Vitro

SEC inhibitor KL-1 (0-100μM, 24-72h) inhibits the growth of DIPG cells and increases apoptosis[1].
SEC inhibitor KL-1 for H3 wild-type astrocytes, the h3g34v mutant glioma cells, and NHA cells with IC50 are 18, 16 and 18μM, respectively[1].
SEC inhibitor KL-1 disrupts SEC, leading to increase occupancy of Pol II near the promoter, which can delay Pol II mutants and slow down Pol II's elongation rate[2].
SEC inhibitor KL-1 (5h) blocks the induction of heat shock-induced genes FOS, HSPD1, HSPE1, and EGR1 in HCT116 cells, impairing the heat shock response[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SEC inhibitor KL-1 Related Antibodies

Cell Viability Assay[1]

Cell Line: SF8628, DIPG-007, SU-DIPG4, SU-DIPG36
Concentration: 0-100 μM
Incubation Time: 24, 72 h
Result: Inhibited the growth of DIPG cells.

Apoptosis Analysis[1]

Cell Line: H3K27M mutant DIPG cells
Concentration: 20, 40 μM
Incubation Time: 72 h
Result: Increased in a dose-dependent manner in Annexin v positive cells, increasing apoptosis.
In Vivo

SEC inhibitor KL-1 (50 mg/kg, administered intraperitoneally, once daily, 15 days) disrupts SEC, which can slow down tumor progression and improve survival rates in MDA231-LM2 tumor mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mouse MDA231-LM2 tumor model[2]
Dosage: 50 mg/kg, once a day, 15 days
Administration: Intraperitoneal injection (i.p.)
Result: Reduced tumor volume and significantly extended survival time of recipient mice.
Molecular Weight

345.78

Formula

C18H16ClNO4
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC1=CC(Cl)=CC=C1C)/C(O)=C/C(C2=CC=CC(OC)=C2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 41.67 mg/mL (120.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8920 mL 14.4601 mL 28.9201 mL
5 mM 0.5784 mL 2.8920 mL 5.7840 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.08 mg/mL (6.02 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (6.02 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 5 mg/mL (14.46 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8920 mL 14.4601 mL 28.9201 mL 72.3003 mL
5 mM 0.5784 mL 2.8920 mL 5.7840 mL 14.4601 mL
10 mM 0.2892 mL 1.4460 mL 2.8920 mL 7.2300 mL
15 mM 0.1928 mL 0.9640 mL 1.9280 mL 4.8200 mL
20 mM 0.1446 mL 0.7230 mL 1.4460 mL 3.6150 mL
25 mM 0.1157 mL 0.5784 mL 1.1568 mL 2.8920 mL
30 mM 0.0964 mL 0.4820 mL 0.9640 mL 2.4100 mL
40 mM 0.0723 mL 0.3615 mL 0.7230 mL 1.8075 mL
50 mM 0.0578 mL 0.2892 mL 0.5784 mL 1.4460 mL
60 mM 0.0482 mL 0.2410 mL 0.4820 mL 1.2050 mL
80 mM 0.0362 mL 0.1808 mL 0.3615 mL 0.9038 mL
100 mM 0.0289 mL 0.1446 mL 0.2892 mL 0.7230 mL
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SEC inhibitor KL-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SEC inhibitor KL-1900308-84-1KL-1SEC inhibitor KL1SEC inhibitor KL 1KL1KL 1Apoptosistumor suppressionSF8628DIPG-007SU-DIPG4SU-DIPG36Inhibitorinhibitorinhibit

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