2. Apoptosis Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Apoptosis Reactive Oxygen Species
  4. SFI003

SFI003 is a SRSF3 inhibitor that drives CRC cell apoptosis via the SRSF3/DHCR24/ROS axis and exhibits potent antitumor effects both in vitro and in vivo.

For research use only. We do not sell to patients.

SFI003 Chemical Structure

SFI003 Chemical Structure

CAS No. : 2361332-90-1

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5 mg USD 215 In-stock
10 mg USD 344 In-stock
25 mg USD 689 In-stock
50 mg USD 1103 In-stock
100 mg USD 1765 In-stock
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Based on 1 publication(s) in Google Scholar

SFI003 Related Antibodies

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Description

SFI003 is a SRSF3 inhibitor that drives CRC cell apoptosis via the SRSF3/DHCR24/ROS axis and exhibits potent antitumor effects both in vitro and in vivo[1].

In Vitro

SFI003 (0-50 μM, 0-72 h) inhibits the proliferation of HCT116 and SW480 cells in a dose- and time-dependent manner with IC50values ​​of 8.78 µM and 48.67 µM, respectively[1].
SFI003 (0-50 μM, 24-72 h) induces apoptosis in HCT-116 and SW480 cells[1].
SFI003 (0-50 μM, 24-72 h) induces ROS generation in both HCT-116 and SW480 cells in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SFI003 Related Antibodies

Western Blot Analysis[1]

Cell Line: HCT-116 and SW480 cells
Concentration: 10, 20, 50 μM
Incubation Time: 24, 48, 72 h
Result: Inhibited the activity of Akt (p-Akt) and mTOR (p-mTOR).
Inhibited DHCR24 protein expression.
In Vivo

SFI003 (p.o., 100, 200 mg/kg; for 2 consecutive weeks) dose-dependently inhibits the growth of HCT-116 and SW480 xenografted SCID mice in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Subcutaneous injection of HCT-116 and SW480 cells into aplastic male SCID mice[1]
Dosage: 100 and 200 mg/kg
Administration: Oral gavage (p.o.) for two weeks
Result: At a dose of 200 mg/kg, the tumors in half of the mice disappeared, and at a dose of 100 mg/kg, the tumors in some mice also disappeared.
Molecular Weight

363.44

Formula

C19H17N5OS
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

COC(C=C1)=CC=C1C2=CSC(N/N=C(C3=NC4=CC=CC=C4N3)\C)=N2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (343.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7515 mL 13.7574 mL 27.5149 mL
5 mM 0.5503 mL 2.7515 mL 5.5030 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7515 mL 13.7574 mL 27.5149 mL 68.7871 mL
5 mM 0.5503 mL 2.7515 mL 5.5030 mL 13.7574 mL
10 mM 0.2751 mL 1.3757 mL 2.7515 mL 6.8787 mL
15 mM 0.1834 mL 0.9172 mL 1.8343 mL 4.5858 mL
20 mM 0.1376 mL 0.6879 mL 1.3757 mL 3.4394 mL
25 mM 0.1101 mL 0.5503 mL 1.1006 mL 2.7515 mL
30 mM 0.0917 mL 0.4586 mL 0.9172 mL 2.2929 mL
40 mM 0.0688 mL 0.3439 mL 0.6879 mL 1.7197 mL
50 mM 0.0550 mL 0.2751 mL 0.5503 mL 1.3757 mL
60 mM 0.0459 mL 0.2293 mL 0.4586 mL 1.1465 mL
80 mM 0.0344 mL 0.1720 mL 0.3439 mL 0.8598 mL
100 mM 0.0275 mL 0.1376 mL 0.2751 mL 0.6879 mL
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SFI003 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SFI0032361332-90-1SFI 003SFI-003ApoptosisReactive Oxygen SpeciesHCT-116SW480colorectal cancerInhibitorinhibitorinhibit

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