2. Vitamin D Related/Nuclear Receptor
  3. Androgen Receptor
  4. SK33

SK33, a trifluoromethylated enobosarm analog, is a potent, and tissue selective anti-androgen. SK33reduces androgen receptor (AR) transcriptional activity.

For research use only. We do not sell to patients.

SK33 Chemical Structure

SK33 Chemical Structure

CAS No. : 1928724-23-5

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5 mg USD 320 In-stock
10 mg USD 490 In-stock
50 mg USD 1350 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

SK33 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SK33, a trifluoromethylated enobosarm analog, is a potent, and tissue selective anti-androgen. SK33reduces androgen receptor (AR) transcriptional activity[1].

Cellular Effect
Cell Line Type Value Description References
CWR22R IC50
2.64 μM
Compound: 30a
Antiproliferative activity against human 22Rv1 cells assessed as reduction in cell viability after 96 hrs by propidium iodide staining based fluorescence assay
Antiproliferative activity against human 22Rv1 cells assessed as reduction in cell viability after 96 hrs by propidium iodide staining based fluorescence assay
[PMID: 27301368]
DU-145 IC50
4.7 μM
Compound: 30a
Antiproliferative activity against human DU145 cells assessed as reduction in cell viability after 96 hrs by propidium iodide staining based fluorescence assay
Antiproliferative activity against human DU145 cells assessed as reduction in cell viability after 96 hrs by propidium iodide staining based fluorescence assay
[PMID: 27301368]
LNCaP IC50
3.01 μM
Compound: 30a
Antiproliferative activity against human LNCAP cells assessed as reduction in cell viability after 96 hrs by propidium iodide staining based fluorescence assay
Antiproliferative activity against human LNCAP cells assessed as reduction in cell viability after 96 hrs by propidium iodide staining based fluorescence assay
[PMID: 27301368]
VCaP IC50
2.47 μM
Compound: 30a
Antiproliferative activity against human VCaP cells assessed as reduction in cell viability after 96 hrs by propidium iodide staining based fluorescence assay
Antiproliferative activity against human VCaP cells assessed as reduction in cell viability after 96 hrs by propidium iodide staining based fluorescence assay
[PMID: 27301368]
In Vitro

In LNCaPAR+?cells, SK33, demonstrates a significantly potent activity with IC50=0.2 μM. SK33 decreases cells entering S-phase in LNCaP cells.?Treatment of LNCaP/BicR cells with increasing concentrations of SK33 for 96 hours results in a dose-dependent response and an inhibition of cell growth, with IC50?of approximately 5 μM for SK33[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SK33 Related Antibodies

Cell Viability Assay[1]

Cell Line: LNCaP/BicR cells
Concentration: 0.1-100 μM
Incubation Time: 96 hours
Result: Results in a dose-dependent response and an inhibition of cell growth.
In Vivo

SK33 (50 mg/kg; s.c.; 24 hours) inhibits AR transcriptional activity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ARE-Luc mice[1]
Dosage: 50 mg/kg
Administration: Subcutaneously; 24 hours
Result: Inhibited AR transcriptional activity.
Molecular Weight

500.31

Formula

C20H13F9N2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=C(C#N)C(C(F)(F)F)=C1)C(C)(O)COC2=CC(C(F)(F)F)=CC(C(F)(F)F)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (199.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9988 mL 9.9938 mL 19.9876 mL
5 mM 0.3998 mL 1.9988 mL 3.9975 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9988 mL 9.9938 mL 19.9876 mL 49.9690 mL
5 mM 0.3998 mL 1.9988 mL 3.9975 mL 9.9938 mL
10 mM 0.1999 mL 0.9994 mL 1.9988 mL 4.9969 mL
15 mM 0.1333 mL 0.6663 mL 1.3325 mL 3.3313 mL
20 mM 0.0999 mL 0.4997 mL 0.9994 mL 2.4985 mL
25 mM 0.0800 mL 0.3998 mL 0.7995 mL 1.9988 mL
30 mM 0.0666 mL 0.3331 mL 0.6663 mL 1.6656 mL
40 mM 0.0500 mL 0.2498 mL 0.4997 mL 1.2492 mL
50 mM 0.0400 mL 0.1999 mL 0.3998 mL 0.9994 mL
60 mM 0.0333 mL 0.1666 mL 0.3331 mL 0.8328 mL
80 mM 0.0250 mL 0.1249 mL 0.2498 mL 0.6246 mL
100 mM 0.0200 mL 0.0999 mL 0.1999 mL 0.4997 mL
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SK33 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SK331928724-23-5SK 33SK-33Androgen ReceptorPC3cellsandrogenprostatecancerLNCaPtissueanaloguesInhibitorinhibitorinhibit

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