SKF 81297

Name: SKF 81297
Brand: MedChemExpress
SKU: HY-12236A
Rating: 4.5 (1 reviews)

Cat. No.: HY-12236A Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SKF 81297 is a potent and selective dopamine D1 receptor agonist.

For research use only. We do not sell to patients.

SKF 81297 Chemical Structure

CAS No. : 71636-61-8

Size	Price	Stock	Quantity
5 mg	USD 85	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 135	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 270	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 460	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of SKF 81297:

SKF 81297 hydrobromide Get quote

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View All Isoforms

Dopamine Receptor D₁ Receptor D₂ Receptor D₃ Receptor D₄ Receptor D₅ Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

SKF 81297 is a potent and selective dopamine D1 receptor agonist^[1].

IC₅₀ & Target

D₁ Receptor

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	1.4 nM Compound: 2; SKF81297	Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay Agonist activity at D5R (unknown origin) expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay	[PMID: 31098001]
HEK293	EC₅₀	360 nM Compound: 2; SKF81297	Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on beta-arrestin2 recruitment by PRESTO-Tango beta-arrestin2 recruitment assay Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on beta-arrestin2 recruitment by PRESTO-Tango beta-arrestin2 recruitment assay	[PMID: 31098001]
HEK293	EC₅₀	4.7 nM Compound: 2; SKF81297	Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay Agonist activity at wild type human D1R expressed in HEK293 cells assessed as effect on cAMP accumulation incubated for 10 mins by Gs-cAMP Glosensor assay	[PMID: 31098001]

In Vivo

SKF 81297 (0.05-0.3 mg/kg, i.m., once) stimulates motor behaviour of MPTP-lesioned monkeys^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Four male rhesus monkeys (Macaca mulatta, 7.0-11.3 kg)^[1]
Dosage:	0.05-0.3 mg/kg
Administration:	Injected intramuscularly (i.m.), once
Result:	Significantly increased rotational behaviour and right-sided hand use in unilateral MPTP-lesioned rhesus monkeys.

Molecular Weight

289.76

Formula

C₁₆H₁₆ClNO₂

CAS No.

71636-61-8

Appearance

Solid

Color

White to yellow

SMILES

OC1=C(O)C=C2C(C3=CC=CC=C3)CNCCC2=C1Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (345.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4511 mL	17.2557 mL	34.5113 mL
5 mM	0.6902 mL	3.4511 mL	6.9023 mL
10 mM	0.3451 mL	1.7256 mL	3.4511 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (258 KB) HNMR (253 KB) RP-HPLC (331 KB) LCMS (138 KB)

Handling Instructions (2659 KB)

References

[1]. Vermeulen RJ, et al. The selective dopamine D1 receptor agonist, SKF 81297, stimulates motor behaviour of MPTP-lesioned monkeys. Eur J Pharmacol. 1993 Apr 22;235(1):143-7. [Content Brief]

[2]. Auger ML, et al. Amelioration of cognitive impairments induced by GABA hypofunction in the male rat prefrontal cortex by direct and indirect dopamine D1 agonists SKF-81297 and d-Govadine. Neuropharmacology. 2020 Jan 1;162:107844. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4511 mL	17.2557 mL	34.5113 mL	86.2783 mL
	5 mM	0.6902 mL	3.4511 mL	6.9023 mL	17.2557 mL
	10 mM	0.3451 mL	1.7256 mL	3.4511 mL	8.6278 mL
	15 mM	0.2301 mL	1.1504 mL	2.3008 mL	5.7519 mL
	20 mM	0.1726 mL	0.8628 mL	1.7256 mL	4.3139 mL
	25 mM	0.1380 mL	0.6902 mL	1.3805 mL	3.4511 mL
	30 mM	0.1150 mL	0.5752 mL	1.1504 mL	2.8759 mL
	40 mM	0.0863 mL	0.4314 mL	0.8628 mL	2.1570 mL
	50 mM	0.0690 mL	0.3451 mL	0.6902 mL	1.7256 mL
	60 mM	0.0575 mL	0.2876 mL	0.5752 mL	1.4380 mL
	80 mM	0.0431 mL	0.2157 mL	0.4314 mL	1.0785 mL
	100 mM	0.0345 mL	0.1726 mL	0.3451 mL	0.8628 mL

SKF 81297 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SKF 8129771636-61-8SKF81297SKF-81297Dopamine ReceptorInhibitorinhibitorinhibit

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SKF 81297

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Complete Stock Solution Preparation Table

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