2. NF-κB
  3. NF-κB
  4. SP-100030

SP-100030 is a potent NF-κB and activator protein-1 (AP-1) double inhibitor (IC50s=50 and 50 nM, respectively). SP-100030 inhibits IL-2, IL-8, and TNF-alpha production in Jurkat and other T cell lines. SP-100030 decreases murine collagen-induced arthritis (CIA).

For research use only. We do not sell to patients.

SP-100030 Chemical Structure

SP-100030 Chemical Structure

CAS No. : 154563-54-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 154 In-stock
Solution
10 mM * 1 mL in DMSO USD 154 In-stock
Solid
5 mg USD 140 In-stock
10 mg USD 220 In-stock
25 mg USD 590 In-stock
50 mg USD 920 In-stock
100 mg USD 1500 In-stock
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Based on 1 publication(s) in Google Scholar

SP-100030 Related Antibodies

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Description

SP-100030 is a potent NF-κB and activator protein-1 (AP-1) double inhibitor (IC50s=50 and 50 nM, respectively). SP-100030 inhibits IL-2, IL-8, and TNF-alpha production in Jurkat and other T cell lines. SP-100030 decreases murine collagen-induced arthritis (CIA)[1][2].

Cellular Effect
Cell Line Type Value Description References
Jurkat IC50
0.05 μM
Compound: 1, SP100030
Inhibition of AP-1-mediated transcriptional activation in human Jurkat cells by luciferase reporter gene assay
Inhibition of AP-1-mediated transcriptional activation in human Jurkat cells by luciferase reporter gene assay
[PMID: 24831826]
Jurkat IC50
0.05 μM
Compound: 1, SP100030
Inhibition of NF-kappaB-mediated transcriptional activation in human Jurkat cells by luciferase reporter gene assay
Inhibition of NF-kappaB-mediated transcriptional activation in human Jurkat cells by luciferase reporter gene assay
[PMID: 24831826]
In Vitro

SP-100030 blocks the production of IL-2 and IL-8 in Jurkat T-cells at the same concentrations as seen in the luciferase assay (IC50≈30 nM)[1].
ELISA and RT-PCR confirmed that IL-2, IL-8, and TNF-alpha production by activated Jurkat and other T cell lines were inhibited by SP100030[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SP-100030 Related Antibodies
In Vivo

SP100030 (10 mg/kg/day; i.p.; day 21 to day 34) significantly decreased arthritis severity[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six- to 8-wk-old male DBA/1J mice (immunized with type II collagen)[2]
Dosage: 10 mg/kg
Administration: I.p.; daily, starting at day 21 to the end of the study on day 34
Result: Significantly decreased arthritis scores compared with controls; Histologic evaluation of the paws from mice treated from day 20 to 34 showed a trend toward decreased inflammation.
Molecular Weight

437.65

Formula

C14H5ClF9N3O
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

FC(F)(F)C1=CC(C(F)(F)F)=CC(NC(C2=CN=C(Cl)N=C2C(F)(F)F)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (114.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2849 mL 11.4247 mL 22.8493 mL
5 mM 0.4570 mL 2.2849 mL 4.5699 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2849 mL 11.4247 mL 22.8493 mL 57.1233 mL
5 mM 0.4570 mL 2.2849 mL 4.5699 mL 11.4247 mL
10 mM 0.2285 mL 1.1425 mL 2.2849 mL 5.7123 mL
15 mM 0.1523 mL 0.7616 mL 1.5233 mL 3.8082 mL
20 mM 0.1142 mL 0.5712 mL 1.1425 mL 2.8562 mL
25 mM 0.0914 mL 0.4570 mL 0.9140 mL 2.2849 mL
30 mM 0.0762 mL 0.3808 mL 0.7616 mL 1.9041 mL
40 mM 0.0571 mL 0.2856 mL 0.5712 mL 1.4281 mL
50 mM 0.0457 mL 0.2285 mL 0.4570 mL 1.1425 mL
60 mM 0.0381 mL 0.1904 mL 0.3808 mL 0.9521 mL
80 mM 0.0286 mL 0.1428 mL 0.2856 mL 0.7140 mL
100 mM 0.0228 mL 0.1142 mL 0.2285 mL 0.5712 mL
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SP-100030 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SP-100030154563-54-9SP100030SP 100030NF-κBNuclear factor-κBNuclear factor-kappaBproductionIL-2IL-8CIAJurkatcellsTimmunosuppressionInhibitorinhibitorinhibit

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