SPR inhibitor 3 (Synonyms: SPRi3)

Name: SPR inhibitor 3
Brand: MedChemExpress
SKU: HY-115510
Rating: 4.5 (1 reviews)

Cat. No.: HY-115510 Purity: 99.84%: FAQs
Data Sheet SDS COA Handling Instructions

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SPR inhibitor 3 (SPRi3) is a potent sepiapterin reductase (SPR) inhibitor. SPR inhibitor 3 (SPRi3) displays high binding affinity to human SPR in a cell-free assay (IC₅₀=74 nM) and efficiently reduces biopterin levels in a cell-based assay (IC₅₀=5.2 μM). SPR inhibitor 3 (SPRi3) reduces neuropathic and inflammatory pain through a reduction of BH4 levels.

For research use only. We do not sell to patients.

SPR inhibitor 3 Chemical Structure

CAS No. : 1292285-54-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 157	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 157	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 143	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 204	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 693	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 748	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

IC₅₀ & Target

IC50: 74 nM (sepiapterin reductase)^[1]

In Vitro

SPR inhibitor 3 (SPRi3) also significantly reduces SPR activity in mouse primary cultures of sensory neurons (IC₅₀=0.45 μM) without affecting the activity of GTP cyclohydroxylase 1 enzyme (GCH1)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

262.30

Formula

C₁₄H₁₈N₂O₃

CAS No.

1292285-54-1

Appearance

Solid

Color

Off-white to gray

SMILES

O=C(NCCC1=C(C)NC2=C1C=C(O)C=C2)COC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (381.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8124 mL	19.0621 mL	38.1243 mL
5 mM	0.7625 mL	3.8124 mL	7.6249 mL
10 mM	0.3812 mL	1.9062 mL	3.8124 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.84%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (248 KB) HNMR (132 KB) RP-HPLC (246 KB) MS (209 KB)

Handling Instructions (2659 KB)

References

[1]. Latremoliere A, et al. Reduction of Neuropathic and Inflammatory Pain through Inhibition of the Tetrahydrobiopterin Pathway. Neuron. 2015 Jun 17;86(6):1393-406. [Content Brief]

[2]. Cronin SJF, et al. The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature. 2018 Nov;563(7732):564-568. [Content Brief]

[3]. Haruki H, et al. Tetrahydrobiopterin Biosynthesis as a Potential Target of the Kynurenine Pathway Metabolite Xanthurenic Acid. J Biol Chem. 2016 Jan 8;291(2):652-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8124 mL	19.0621 mL	38.1243 mL	95.3107 mL
	5 mM	0.7625 mL	3.8124 mL	7.6249 mL	19.0621 mL
	10 mM	0.3812 mL	1.9062 mL	3.8124 mL	9.5311 mL
	15 mM	0.2542 mL	1.2708 mL	2.5416 mL	6.3540 mL
	20 mM	0.1906 mL	0.9531 mL	1.9062 mL	4.7655 mL
	25 mM	0.1525 mL	0.7625 mL	1.5250 mL	3.8124 mL
	30 mM	0.1271 mL	0.6354 mL	1.2708 mL	3.1770 mL
	40 mM	0.0953 mL	0.4766 mL	0.9531 mL	2.3828 mL
	50 mM	0.0762 mL	0.3812 mL	0.7625 mL	1.9062 mL
	60 mM	0.0635 mL	0.3177 mL	0.6354 mL	1.5885 mL
	80 mM	0.0477 mL	0.2383 mL	0.4766 mL	1.1914 mL
	100 mM	0.0381 mL	0.1906 mL	0.3812 mL	0.9531 mL

SPR inhibitor 3 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SPR inhibitor 31292285-54-1SPRi3SPR inhibitor3SPR inhibitor-3SPRi 3SPRi-3OthersInhibitorinhibitorinhibit

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SPR inhibitor 3 (Synonyms: SPRi3)

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SPR inhibitor 3 Related Antibodies

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Biological Activity

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SPR inhibitor 3 Related Antibodies

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Complete Stock Solution Preparation Table

SPR inhibitor 3 Related Classifications

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