SR1555

Name: SR1555
Brand: MedChemExpress
SKU: HY-120785
Rating: 4.5 (1 reviews)

Cat. No.: HY-120785 Purity: 99.69%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SR1555 is a specific retinoic acid receptor-related orphan nuclear receptor γ (RORγ) inverse agonist with an IC₅₀ value of 1 μM. SR1555 not only inhibits TH17 cell development and function but also increases the frequency of T regulatory cells, as well as inhibits the expression of IL-17. SR1555 can be used for researching autoimmune diseases.

For research use only. We do not sell to patients.

SR1555 Chemical Structure

CAS No. : 1386439-51-5

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 248	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 890	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1470	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of SR1555:

SR1555 hydrochloride Get quote

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RORγ RORα RORβ

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

RORγ

1 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	1.5 μM Compound: 14, SR1555	Agonist activity at RoRc-LBD (unknown origin) expressed in HEK293 cells assessed as transcriptional activity by GAL4 reporter assay Agonist activity at RoRc-LBD (unknown origin) expressed in HEK293 cells assessed as transcriptional activity by GAL4 reporter assay	[PMID: 24502334]
HEK-293T	IC₅₀	1.5 μM Compound: SR1555	Inhibition of GAL4-fused ROR gamma (unknown origin) expressed in HEK293T cells assessed as suppression of transcriptional activity after 20 hrs Inhibition of GAL4-fused ROR gamma (unknown origin) expressed in HEK293T cells assessed as suppression of transcriptional activity after 20 hrs	10.1039/C3MD00005B

In Vitro

SR1555 (10 μM; 24 h) inhibits Il17a gene expression by greater than 70%in EL4 cells^[1].
SR1555 (10 μM; 24 h) inhibits IL-17 protein expression, inhibits T_H17 cell differentiation and function, and does not induce cell death during T_H17 cell differentiation^[1].
SR1555 (10 μM; 24 h) effectively increases the gene expression of Foxp3 while simultaneously suppressing the gene expression of Rorγt^[1].
SR1555 increases the frequency of T regulatory cells as evidenced by the almost 2-fold increase in the expression of Foxp3+ T cells in the splenocyte culture^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	EL4 cells
Concentration:	10 μM
Incubation Time:	24 h
Result:	Inhibited Il17a gene expression by greater than 70%.

Cell Differentiation Assay^[1]

Cell Line:	Splenocytes (cultured under T_H17 polarizing conditions for 3 days)
Concentration:	10 μM
Incubation Time:	24 h
Result:	Inhibited IL-17 protein expression, inhibited T_H17 cell differentiation and function, and did not induce cell death during T_H17 cell differentiation.

RT-PCR^[1]

Cell Line:	Splenocytes (cultured under T regulatory cell polarizing conditions for 3 days)
Concentration:	10 μM
Incubation Time:	24 h
Result:	Effectively increased the gene expression of Foxp3 while simultaneously suppressing the gene expression of Rorγt.

Molecular Weight

460.41

Formula

C₂₂H₂₂F₆N₂O₂

CAS No.

1386439-51-5

Appearance

Solid

Color

White to light yellow

SMILES

CC(N1CCN(CC2=CC=C(C3=CC=C(C(C(F)(F)F)(O)C(F)(F)F)C=C3)C=C2)CC1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (217.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1720 mL	10.8599 mL	21.7198 mL
5 mM	0.4344 mL	2.1720 mL	4.3440 mL
10 mM	0.2172 mL	1.0860 mL	2.1720 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (247 KB) HNMR (138 KB) RP-HPLC (138 KB) LCMS (387 KB)

Handling Instructions (2659 KB)

References

[1]. Solt LA, Kumar N, He Y, Kamenecka TM, Griffin PR, Burris TP. Identification of a selective RORγ ligand that suppresses T(H)17 cells and stimulates T regulatory cells. ACS Chem Biol. 2012 Sep 21;7(9):1515-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1720 mL	10.8599 mL	21.7198 mL	54.2994 mL
	5 mM	0.4344 mL	2.1720 mL	4.3440 mL	10.8599 mL
	10 mM	0.2172 mL	1.0860 mL	2.1720 mL	5.4299 mL
	15 mM	0.1448 mL	0.7240 mL	1.4480 mL	3.6200 mL
	20 mM	0.1086 mL	0.5430 mL	1.0860 mL	2.7150 mL
	25 mM	0.0869 mL	0.4344 mL	0.8688 mL	2.1720 mL
	30 mM	0.0724 mL	0.3620 mL	0.7240 mL	1.8100 mL
	40 mM	0.0543 mL	0.2715 mL	0.5430 mL	1.3575 mL
	50 mM	0.0434 mL	0.2172 mL	0.4344 mL	1.0860 mL
	60 mM	0.0362 mL	0.1810 mL	0.3620 mL	0.9050 mL
	80 mM	0.0271 mL	0.1357 mL	0.2715 mL	0.6787 mL
	100 mM	0.0217 mL	0.1086 mL	0.2172 mL	0.5430 mL

SR1555 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SR15551386439-51-5SR 1555SR-1555RORRAR-related orphan receptorRORγTH17 cellsT regulatory cellsIL-17EL4 cellssplenocytesautoimmune diseasesInhibitorinhibitorinhibit

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SR1555

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Other Forms of SR1555:

SR1555 Related Antibodies

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Customer Review

SR1555 Related Antibodies

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Complete Stock Solution Preparation Table

SR1555 Related Classifications

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