STAT3-IN-12

Name: STAT3-IN-12
Brand: MedChemExpress
SKU: HY-150538
Rating: 4.5 (1 reviews)

Cat. No.: HY-150538 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

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STAT3-IN-12 is a potent STAT3 signal inhibitor that can inhibit IL-6 induced JAK/STAT3 signalling pathway activation. STAT3-IN-12 inhibits cancer cell growth, migration, and induce cell apoptosis as well as cycle arrest. STAT3-IN-12 can be used in cancer-related research, such as hepatocellular carcinoma (HCC) and oesophageal carcinoma.

For research use only. We do not sell to patients.

STAT3-IN-12 Chemical Structure

CAS No. : 2980758-31-2

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1550	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

STAT3

Cellular Effect

Cell Line	Type	Value	Description	References
ECa-109 cell line	IC₅₀	3.63 μM Compound: 24	Antitumor activity against human EC109 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antitumor activity against human EC109 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35504209]
HepG2	IC₅₀	4.3 μM Compound: 24	Antitumor activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antitumor activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35504209]

In Vitro

STAT3-IN-12 (compound 24, 0-10 μM approximately, 72 h) inhibits cancer cell growth and migration in HepG2 and EC109 cells^[1].
STAT3-IN-12 (0-20 μM, 16 h) binds to the STAT3 protein and inhibits IL-6-mediated STAT3 phosphorylation, also inhibts STAT3 nuclear localization and dimerization in EC109 and HepG2 cells^[1].
STAT3-IN-12 (0-20 μM, 48 h) induces cell apoptosis as well as cycle arrest in HepG2 and EC109 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HepG2 and EC109 cells
Concentration:	0, 1.25, 2.5, 5 and 10 μM.
Incubation Time:	72 h
Result:	Inhibited cancer cell growth with IC₅₀ values of 4.32 and 3.63 μM.

Cell Migration Assay ^[1]

Cell Line:	HepG2 and EC109 cells
Concentration:	0-10 μM
Incubation Time:	24 h
Result:	Inhibited cancer cell migration.

Western Blot Analysis^[1]

Cell Line:	HepG2 and EC109 cells
Concentration:	0, 2.5, 5, 10 and 20 μM
Incubation Time:	16 h
Result:	Inhibited phosphorylation of STAT3 tyrosine 705 with high selectivity.

In Vivo

STAT3-IN-12 (compound 24, intraperitoneal injection, 20, 40 mg/kg, daily for 24 days) displays obvious antitumor activity in a mouse HepG2 cell xenograft tumor model without no obvious toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	HepG2 cell xenograft tumor model^[1]
Dosage:	20, 40 mg/kg, daily for 24 days
Administration:	Intraperitoneal injection
Result:	Inhibited tumor growth without affecting the body weight.

Molecular Weight

454.56

Formula

C₂₈H₃₀N₄O₂

CAS No.

2980758-31-2

Appearance

Solid

Color

Off-white to light yellow

SMILES

CCCN1C2=C(C=C3C(N(CCC)C(C4=CC=CC(OC)=C4)=N3)=C2)N=C1C5=CC=CC(OC)=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (219.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1999 mL	10.9996 mL	21.9993 mL
5 mM	0.4400 mL	2.1999 mL	4.3999 mL
10 mM	0.2200 mL	1.1000 mL	2.1999 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (247 KB) HNMR (147 KB) RP-HPLC (171 KB) LCMS (387 KB)

Handling Instructions (2659 KB)

References

[1]. Yi-Chen Liu, et al. Benzobis(imidazole) derivatives as STAT3 signal inhibitors with antitumor activity. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1999 mL	10.9996 mL	21.9993 mL	54.9982 mL
	5 mM	0.4400 mL	2.1999 mL	4.3999 mL	10.9996 mL
	10 mM	0.2200 mL	1.1000 mL	2.1999 mL	5.4998 mL
	15 mM	0.1467 mL	0.7333 mL	1.4666 mL	3.6665 mL
	20 mM	0.1100 mL	0.5500 mL	1.1000 mL	2.7499 mL
	25 mM	0.0880 mL	0.4400 mL	0.8800 mL	2.1999 mL
	30 mM	0.0733 mL	0.3667 mL	0.7333 mL	1.8333 mL
	40 mM	0.0550 mL	0.2750 mL	0.5500 mL	1.3750 mL
	50 mM	0.0440 mL	0.2200 mL	0.4400 mL	1.1000 mL
	60 mM	0.0367 mL	0.1833 mL	0.3667 mL	0.9166 mL
	80 mM	0.0275 mL	0.1375 mL	0.2750 mL	0.6875 mL
	100 mM	0.0220 mL	0.1100 mL	0.2200 mL	0.5500 mL

STAT3-IN-12 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

STAT3-IN-122980758-31-2STATApoptosisBenzobis derivativesapoptosis HepG2EC109xenograft tumor modelanticancerInhibitorinhibitorinhibit

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STAT3-IN-12

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STAT3-IN-12 Related Antibodies

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Complete Stock Solution Preparation Table

STAT3-IN-12 Related Classifications

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