STING agonist-3 trihydrochloride

Name: STING agonist-3 trihydrochloride
Brand: MedChemExpress
SKU: HY-103665A
Rating: 5.0 (9 reviews)

Cat. No.: HY-103665A Purity: 98.36%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

STING agonist-3 trihydrochloride, extracted from patent WO2017175147A1 (example 10), is a selective and non-nucleotide small-molecule STING agonist with a pEC₅₀ and pEC₅₀ of 7.5 and 9.5, respectively. STING agonist-3 trihydrochloride has durable anti-tumor effect and tremendous potential to improve treatment of cancer.

For research use only. We do not sell to patients.

STING agonist-3 trihydrochloride Chemical Structure

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of STING agonist-3 trihydrochloride:

STING agonist-3 Get quote

7 Publications Citing Use of MCE STING agonist-3 trihydrochloride

•SSRN. 2023 Nov 9.

: STING agonist-3 trihydrochloride purchased from MedChemExpress. Usage Cited in: Nature. 2022 Oct;610(7933):761-767. [Abstract]; Incubate HeLa cGAS KO cells with NC siRNA or siRNAs targeting AP-1σ1 and AP-1 σ3 for 3 days and reconstitute with empty vector or HA-tagged AP-1σ1 are treated with 2.5 µM diABZI (STING agonist-3) for 0, 2, 4 h before analysis by western blot.

: STING agonist-3 trihydrochloride purchased from MedChemExpress. Usage Cited in: Nature. 2022 Oct;610(7933):761-767. [Abstract]; STING and pSTING are enriched in CCVs obtained from STING agonist-3 (diABZI; 2.5 μM; for 2 hours)-stimulated cells, but not in those obtained from unstimulated cells.

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

STING^[1]

In Vitro

STING agonist-3 trihydrochloride exhibits a pEC₅₀ value of 7.5 in activation of STING in cells, this assay is determined using a luciferase reporter assay in human embryonic kidney cells (HEK293T) co-transfected with plasmids expressing STING and the enzyme firefly luciferase driven by the interferon stimulated response element promoter^[1].
STING agonist-3 trihydrochloride exhibits a pIC₅₀ value of 9.5 in FRET assay. This is a competition binding assay which aims to determine the binding potency of molecules to the C-terminal Domain (CTD) of human STING^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

860.19

Formula

C₃₇H₄₅Cl₃N₁₂O₆

Appearance

Solid

SMILES

O=C(N)C1=CC=C(N(C(NC(C2=CC(C)=NN2CC)=O)=N3)C/C=C/CN4C5=C(N=C4NC(C6=CC(C)=NN6CC)=O)C=C(C(N)=O)C=C5OCCCO)C3=C1.Cl.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (23.25 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1625 mL	5.8127 mL	11.6253 mL
5 mM	0.2325 mL	1.1625 mL	2.3251 mL
10 mM	0.1163 mL	0.5813 mL	1.1625 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2 mg/mL (2.33 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2 mg/mL (2.33 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2 mg/mL (2.33 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.36%

Select Batch:

Data Sheet (305 KB) SDS (251 KB)

COA (265 KB) HNMR (163 KB) RP-HPLC (258 KB) LCMS (262 KB)

Handling Instructions (2659 KB)

References

[1]. Adam Kenneth, et al. Heterocyclic amides useful as protein modulators.patent WO2017175147A1

[2]. Ramanjulu JM, Pesiridis GS, Yang J, et al. Design of amidobenzimidazole STING receptor agonists with systemic activity [published correction appears in Nature. 2019 Jun;570(7761):E53. doi: 10.1038/s41586-019-1265-5]. Nature. 2018;564(7736):439-443. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1625 mL	5.8127 mL	11.6253 mL	29.0633 mL
	5 mM	0.2325 mL	1.1625 mL	2.3251 mL	5.8127 mL
	10 mM	0.1163 mL	0.5813 mL	1.1625 mL	2.9063 mL
	15 mM	0.0775 mL	0.3875 mL	0.7750 mL	1.9376 mL
	20 mM	0.0581 mL	0.2906 mL	0.5813 mL	1.4532 mL