2. Immunology/Inflammation
  3. STING
  4. STING agonist-3

STING agonist-3, extracted from patent WO2017175147A1 (example 10), is a selective and non-nucleotide small-molecule STING agonist with a pEC50 and pIC50 of 7.5 and 9.5, respectively. STING agonist-3 has durable anti-tumor effect and tremendous potential to improve treatment of cancer.

For research use only. We do not sell to patients.

STING agonist-3 Chemical Structure

STING agonist-3 Chemical Structure

CAS No. : 2138299-29-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 578 In-stock
Solution
10 mM * 1 mL in DMSO USD 578 In-stock
Solid
1 mg USD 133 In-stock
5 mg USD 400 In-stock
10 mg USD 700 In-stock
25 mg USD 1100 In-stock
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100 mg   Get quote  

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of STING agonist-3:

STING agonist-3 Related Antibodies

Top Publications Citing Use of Products

    STING agonist-3 purchased from MedChemExpress. Usage Cited in: Nature. 2022 Oct;610(7933):761-767.  [Abstract]

    Incubate HeLa cGAS KO cells with NC siRNA or siRNAs targeting AP-1σ1 and AP-1 σ3 for 3 days and reconstitute with empty vector or HA-tagged AP-1σ1 are treated with 2.5 µM diABZI (STING agonist-3) for 0, 2, 4 h before analysis by western blot.

    STING agonist-3 purchased from MedChemExpress. Usage Cited in: Nature. 2022 Oct;610(7933):761-767.  [Abstract]

    STING and pSTING are enriched in CCVs obtained from STING agonist-3 (diABZI; 2.5 μM; for 2 hours)-stimulated cells, but not in those obtained from unstimulated cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    STING agonist-3, extracted from patent WO2017175147A1 (example 10), is a selective and non-nucleotide small-molecule STING agonist with a pEC50 and pIC50 of 7.5 and 9.5, respectively. STING agonist-3 has durable anti-tumor effect and tremendous potential to improve treatment of cancer[1].

    In Vitro

    STING agonist-3 exhibits a pEC50 value of 7.5 in activation of STING in cells, this assay is determined using a luciferase reporter assay in human embryonic kidney cells (HEK293T) co-transfected with plasmids expressing STING and the enzyme firefly luciferase driven by the interferon stimulated response element promoter[1].
    STING agonist-3 exhibits a pIC50 value of 9.5 in FRET assay. This is a competition binding assay which aims to determine the binding potency of molecules to the C-terminal Domain (CTD) of human STING[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    STING agonist-3 Related Antibodies
    Molecular Weight

    750.81

    Formula

    C37H42N12O6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(N)C1=CC=C(N(C(NC(C2=CC(C)=NN2CC)=O)=N3)C/C=C/CN4C5=C(N=C4NC(C6=CC(C)=NN6CC)=O)C=C(C(N)=O)C=C5OCCCO)C3=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 41.67 mg/mL (55.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3319 mL 6.6595 mL 13.3189 mL
    5 mM 0.2664 mL 1.3319 mL 2.6638 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (2.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.08 mg/mL (2.77 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Dosing volume
    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    % 
    +
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    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.90%

    COA (264 KB) LCMS (92 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.3319 mL 6.6595 mL 13.3189 mL 33.2974 mL
    5 mM 0.2664 mL 1.3319 mL 2.6638 mL 6.6595 mL
    10 mM 0.1332 mL 0.6659 mL 1.3319 mL 3.3297 mL
    15 mM 0.0888 mL 0.4440 mL 0.8879 mL 2.2198 mL
    20 mM 0.0666 mL 0.3330 mL 0.6659 mL 1.6649 mL
    25 mM 0.0533 mL 0.2664 mL 0.5328 mL 1.3319 mL
    30 mM 0.0444 mL 0.2220 mL 0.4440 mL 1.1099 mL
    40 mM 0.0333 mL 0.1665 mL 0.3330 mL 0.8324 mL
    50 mM 0.0266 mL 0.1332 mL 0.2664 mL 0.6659 mL
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    STING agonist-3 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    STING agonist-32138299-29-1STING agonist3STING agonist 3STINGStimulator of Interferon GenesTMEM173MITAERISMPYSstingagonisttumorcancerInhibitorinhibitorinhibit

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