2. Peptides
  3. Peptide and Derivatives
  4. Inhibitors and Substrates
  5. Enzyme Substrates
  6. Suc-AAPF-AMC

Suc-AAPF-AMC  (Synonyms: Suc-Ala-Ala-Pro-Phe-AMC)

Cat. No.: HY-137811 Purity: 99.07%
COA Handling Instructions

Molarity Calculator

Suc-AAPF-AMC is an α-chymotrypsin fluorogenic substrate for the quantitative determination of chymotrypsin activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Suc-AAPF-AMC Chemical Structure

Suc-AAPF-AMC Chemical Structure

CAS No. : 88467-45-2

Size Price Stock Quantity
5 mg USD 75 In-stock
10 mg USD 125 In-stock
25 mg USD 250 In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Suc-AAPF-AMC Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Suc-AAPF-AMC

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Suc-AAPF-AMC is an α-chymotrypsin fluorogenic substrate for the quantitative determination of chymotrypsin activity[1].

Molecular Weight

661.70

Formula

C34H39N5O9
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

{Suc}-Ala-Ala-Pro-Phe-{AMC}
Sequence Shortening

{Suc}-AAPF-{AMC}
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (151.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5113 mL 7.5563 mL 15.1126 mL
5 mM 0.3023 mL 1.5113 mL 3.0225 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.78 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.78 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5113 mL 7.5563 mL 15.1126 mL 37.7815 mL
5 mM 0.3023 mL 1.5113 mL 3.0225 mL 7.5563 mL
10 mM 0.1511 mL 0.7556 mL 1.5113 mL 3.7781 mL
15 mM 0.1008 mL 0.5038 mL 1.0075 mL 2.5188 mL
20 mM 0.0756 mL 0.3778 mL 0.7556 mL 1.8891 mL
25 mM 0.0605 mL 0.3023 mL 0.6045 mL 1.5113 mL
30 mM 0.0504 mL 0.2519 mL 0.5038 mL 1.2594 mL
40 mM 0.0378 mL 0.1889 mL 0.3778 mL 0.9445 mL
50 mM 0.0302 mL 0.1511 mL 0.3023 mL 0.7556 mL
60 mM 0.0252 mL 0.1259 mL 0.2519 mL 0.6297 mL
80 mM 0.0189 mL 0.0945 mL 0.1889 mL 0.4723 mL
100 mM 0.0151 mL 0.0756 mL 0.1511 mL 0.3778 mL
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Suc-AAPF-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Suc-AAPF-AMC88467-45-2Suc-Ala-Ala-Pro-Phe-AMCOthersα-chymotrypsinchymotrypsinInhibitorinhibitorinhibit

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