2. Antibody-drug Conjugate/ADC Related
  3. ADC Cytotoxin
  4. T01-1

T01-1 is an anticancer agent (camptothecin derivative) with good anti-proliferative activity.

For research use only. We do not sell to patients.

T01-1 Chemical Structure

T01-1 Chemical Structure

CAS No. : 2356229-14-4

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Customer Review

Based on 1 publication(s) in Google Scholar

T01-1 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

T01-1 is an anticancer agent (camptothecin derivative) with good anti-proliferative activity[1].

In Vitro

T01-1 (0-50 nM; 3 days) anti-proliferative activity in HCC1806 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

T01-1 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: HCC1806 cells
Concentration: 0-50 nM
Incubation Time: 3 days
Result: Inhibited cell proliferation with an EC50 value of 6.7 nM.
In Vivo

T01-1 (1 mg/kg; i.v.; single) shows T1/2 values of 2.78 and 1.97 h in female and male rats, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague Dawley rat (half male and half female)[1].
Dosage: 1 mg/kg
Administration: Intravenous injection; single
Result: 1.19 Pharmacokinetic Parameters of T01-1 in Sprague Dawley rat [1].
IV (1 mg/kg)
Female Male
AUCLast (ng/mL•h) 437 214
Cmax (ng/mL) 838 625
MRTINF (h) 2.81 0.44
T1/2 (h) 2.78 1.97
Tmax (h) 0.08 0.08
Molecular Weight

511.59

Formula

C26H29N3O6S
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1N2C(C3=NC4=CC=CC=C4C(CCN(C(C)C)S(C)(=O)=O)=C3C2)=CC5=C1COC([C@]5(O)CC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (48.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9547 mL 9.7735 mL 19.5469 mL
5 mM 0.3909 mL 1.9547 mL 3.9094 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.80%

COA (255 KB) HNMR (343 KB) LCMS (98 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9547 mL 9.7735 mL 19.5469 mL 48.8673 mL
5 mM 0.3909 mL 1.9547 mL 3.9094 mL 9.7735 mL
10 mM 0.1955 mL 0.9773 mL 1.9547 mL 4.8867 mL
15 mM 0.1303 mL 0.6516 mL 1.3031 mL 3.2578 mL
20 mM 0.0977 mL 0.4887 mL 0.9773 mL 2.4434 mL
25 mM 0.0782 mL 0.3909 mL 0.7819 mL 1.9547 mL
30 mM 0.0652 mL 0.3258 mL 0.6516 mL 1.6289 mL
40 mM 0.0489 mL 0.2443 mL 0.4887 mL 1.2217 mL
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T01-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

T01-12356229-14-4ADC CytotoxinADC PayloadHCC1806camptothecin derivativecancertumourantiproliferativeInhibitorinhibitorinhibit

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