2. Apoptosis
  3. Apoptosis Necroptosis
  4. Theaflavin-3'-gallate

Theaflavin-3'-gallate is a monomer of theaflavins found in black tea, which has anti-UVB damage activity and inhibits cell apoptosis and necroptosis. Theaflavin-3'-gallate acts as a pro-oxidant and induces oxidative stress in cancer cells, inhibiting xanthine oxidase (OX), with an IC50 of 7.6 μM.

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Theaflavin-3'-gallate Chemical Structure

Theaflavin-3'-gallate Chemical Structure

CAS No. : 28543-07-9

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Based on 1 publication(s) in Google Scholar

Other Forms of Theaflavin-3'-gallate:

Theaflavin-3'-gallate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Theaflavin-3'-gallate

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Theaflavin-3'-gallate is a monomer of theaflavins found in black tea, which has anti-UVB damage activity and inhibits cell apoptosis and necroptosis. Theaflavin-3'-gallate acts as a pro-oxidant and induces oxidative stress in cancer cells, inhibiting xanthine oxidase (OX), with an IC50 of 7.6 μM[1][2][3].

IC50 & Target

OX

7.6 μM (IC50)

In Vitro

Theaflavin-3'-gallate (0-500 μM, 24 h) has a toxic effect on HF-1, HSC-2, and CAL27 cells[1].
Theaflavin-3'-gallate (0-250 μM, 3 h) generates a high amount of peroxide at pH 7.4, and reduces the GSH levels in HF-1 and CAL27 cells[1].
Theaflavin-3'-gallate is the most effective exogenous H2O2 scavenger, showing superoxide scavenging activity in HL-60 cells with an IC50 of 15.6 μM[2].
Theaflavin-3'-gallate (0.1-40 μM, 6 h) has a protective effect against UV-induced damage in HaCaT cells, with an EC50 of 0.483 μM[3].
Theaflavin-3'-gallate (5-40 μM, 6 h) protects against apoptosis and necrosis induced by UVB in HaCaT cells, inhibiting oxidative stress and preserving mitochondrial membrane potential[3].
Theaflavin-3'-gallate (5-40 μM, 6 h) downregulates the NF-κB inflammatory pathway in UV-irradiated HaCaT cells, inhibits the formation of cytotoxic aggregates, and protects the structure of biomacromolecules[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Theaflavin-3'-gallate Related Antibodies

Cell Viability Assay[1]

Cell Line: GN46, HF-1, HSC-2, CAL27
Concentration: 0, 100, 200, 300, 400, 500 μM
Incubation Time: 24 h
Result: Showed initial toxicity to CAL27, HSC-2, and HF-1 cells at concentrations of 250 μM, 350 μM, and 400 μM respectively, but the initial toxicity to GN46 cells is uncertain.

Cell Viability Assay[3]

Cell Line: HaCaT
Concentration: 0.1, 0.25, 0.5, 1, 5, 10, 20, 40 μM
Incubation Time: 6 h
Result: Enhanced cell vitality, showing dose-dependence.

Western Blot Analysis[3]

Cell Line: HaCaT
Concentration: 5,10,20,40μM
Incubation Time: 6 h
Result: Reduced the formation of cytotoxic proteins and inhibited their pathways. Lowered the phosphorylation levels of IKKβ and IκBα, reducing the nuclear translocation of NF-κB and the expression of IL-6.
Molecular Weight

716.60

Formula

C36H28O16
CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

O=C(O[C@H]1[C@@H](C2=C(C=C([C@@H]3[C@H](O)CC4=C(O)C=C(O)C=C4O3)C=C(O)C5=O)C5=C(O)C(O)=C2)OC6=CC(O)=CC(O)=C6C1)C7=CC(O)=C(O)C(O)=C7
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (69.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3955 mL 6.9774 mL 13.9548 mL
5 mM 0.2791 mL 1.3955 mL 2.7910 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.68%

COA (258 KB) HNMR (373 KB) CNMR (356 KB) RP-HPLC (324 KB) MS (106 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3955 mL 6.9774 mL 13.9548 mL 34.8870 mL
5 mM 0.2791 mL 1.3955 mL 2.7910 mL 6.9774 mL
10 mM 0.1395 mL 0.6977 mL 1.3955 mL 3.4887 mL
15 mM 0.0930 mL 0.4652 mL 0.9303 mL 2.3258 mL
20 mM 0.0698 mL 0.3489 mL 0.6977 mL 1.7443 mL
25 mM 0.0558 mL 0.2791 mL 0.5582 mL 1.3955 mL
30 mM 0.0465 mL 0.2326 mL 0.4652 mL 1.1629 mL
40 mM 0.0349 mL 0.1744 mL 0.3489 mL 0.8722 mL
50 mM 0.0279 mL 0.1395 mL 0.2791 mL 0.6977 mL
60 mM 0.0233 mL 0.1163 mL 0.2326 mL 0.5814 mL
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Theaflavin-3'-gallate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Theaflavin-3'-gallate28543-07-9ApoptosisNecroptosisPromoters of oxidationoxidative stressapoptosisInhibitorinhibitorinhibit

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