Theasaponin E2

Cat. No.: HY-N12153 Purity: 98.76%: FAQs
Data Sheet SDS COA Handling Instructions

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Theasaponin E2 shows cytotoxicity against K562 and HL60 cells (IC₅₀: 14.7 μg/mL). Theasaponin E2 can be isolated from Camellia sinensis.

For research use only. We do not sell to patients.

Theasaponin E2 Chemical Structure

CAS No. : 220114-30-7

Size	Price	Stock	Quantity
1 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 430	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Description

Theasaponin E2 shows cytotoxicity against K562 and HL60 cells (IC₅₀: 14.7 μg/mL). Theasaponin E2 can be isolated from Camellia sinensis^[1].

Molecular Weight

1231.33

Formula

C₅₉H₉₀O₂₇

CAS No.

220114-30-7

Appearance

Solid

Color

White to off-white

SMILES

CC(OC[C@]([C@@H](C[C@]12C)O)([C@H]([C@@H]3OC(/C(C)=C\C)=O)O)[C@](CC3(C)C)([H])C1=CC[C@@]4([H])[C@]2(CC[C@]([C@@](C)5C=O)([H])[C@@]4(CC[C@@H]5O[C@H](O[C@@H]6C(O)=O)[C@@H]([C@H]([C@@H]6O)O[C@H](OC[C@@H]7O)[C@@H]([C@H]7O)O[C@H](OC[C@H]8O)[C@@H]([C@H]8O)O)O[C@@H]([C@@H]([C@H]9O)O)O[C@@H]([C@@H]9O)CO)C)C)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (162.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8121 mL	4.0607 mL	8.1213 mL
5 mM	0.1624 mL	0.8121 mL	1.6243 mL
10 mM	0.0812 mL	0.4061 mL	0.8121 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (4.06 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (4.06 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (4.06 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.76%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (256 KB) HNMR (231 KB) CNMR (264 KB) RP-HPLC (162 KB) MS (160 KB)

Handling Instructions (2659 KB)

References

[1]. Li N, et al. Phytochemical analysis of the triterpenoids with cytotoxicity and QR inducing properties from the total tea seed saponin of Camellia sinensis. Fitoterapia. 2013 Jan;84:321-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.8121 mL	4.0607 mL	8.1213 mL	20.3033 mL
	5 mM	0.1624 mL	0.8121 mL	1.6243 mL	4.0607 mL
	10 mM	0.0812 mL	0.4061 mL	0.8121 mL	2.0303 mL
	15 mM	0.0541 mL	0.2707 mL	0.5414 mL	1.3536 mL
	20 mM	0.0406 mL	0.2030 mL	0.4061 mL	1.0152 mL
	25 mM	0.0325 mL	0.1624 mL	0.3249 mL	0.8121 mL
	30 mM	0.0271 mL	0.1354 mL	0.2707 mL	0.6768 mL
	40 mM	0.0203 mL	0.1015 mL	0.2030 mL	0.5076 mL
	50 mM	0.0162 mL	0.0812 mL	0.1624 mL	0.4061 mL
	60 mM	0.0135 mL	0.0677 mL	0.1354 mL	0.3384 mL
	80 mM	0.0102 mL	0.0508 mL	0.1015 mL	0.2538 mL
	100 mM	0.0081 mL	0.0406 mL	0.0812 mL	0.2030 mL

Theasaponin E2 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Theasaponin E2220114-30-7Theasaponin E 2Theasaponin E-2OtherscytotoxicityK562HL60tumorInhibitorinhibitorinhibit

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