TP0586532

Cat. No.: HY-131981 Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TP0586532 is a non-hydroxamate LpxC inhibitor (IC₅₀=0.101 μM). TP0586532 as a compound with a low cardiovascular risk that is effective against K. pneumoniae, including resistant strains. TP0586532 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

TP0586532 Chemical Structure

CAS No. : 2427584-96-9

Size	Price	Stock
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 418	Ask For Quote & Lead Time
Solution
10 mM * 1 mL in DMSO	USD 418	Ask For Quote & Lead Time
Solid
1 mg	USD 152	Ask For Quote & Lead Time
5 mg	USD 380	Ask For Quote & Lead Time
10 mg	USD 600	Ask For Quote & Lead Time
25 mg	USD 1150	Ask For Quote & Lead Time
50 mg	USD 1800	Ask For Quote & Lead Time
100 mg	USD 2850	Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

1 Publications Citing Use of MCE TP0586532

•Bioorg Chem. 2024 Apr 29:147:107412. [Abstract]

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Purity & Documentation
References
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Description

TP0586532 is a non-hydroxamate LpxC inhibitor (IC₅₀=0.101 μM). TP0586532 as a compound with a low cardiovascular risk that is effective against K. pneumoniae, including resistant strains^[1]. TP0586532 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC₅₀ & Target

IC50: 0.101 μM (non-hydroxamate LpxC)^[1]

In Vitro

TP0586532 as a compound with a low cardiovascular risk that is effective against K. pneumoniae, including resistant strains^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

460.52

Formula

C₂₆H₂₈N₄O₄

CAS No.

2427584-96-9

Appearance

Solid

Color

White to off-white

SMILES

C[C@H](O)C1=NC=CN1CC2=NOC(C(C=C3)=CC=C3C#C[C@H]4[C@@]5([H])[C@]4([H])CN(C5)CCCC(O)=O)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (108.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1715 mL	10.8573 mL	21.7146 mL
5 mM	0.4343 mL	2.1715 mL	4.3429 mL
10 mM	0.2171 mL	1.0857 mL	2.1715 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (244 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Ushiyama F, et al. Lead optimization of 2-hydroxymethyl imidazoles as non-hydroxamate LpxC inhibitors: Discovery of TP0586532. Bioorg Med Chem. 2021;30:115964. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1715 mL	10.8573 mL	21.7146 mL	54.2865 mL
	5 mM	0.4343 mL	2.1715 mL	4.3429 mL	10.8573 mL
	10 mM	0.2171 mL	1.0857 mL	2.1715 mL	5.4286 mL
	15 mM	0.1448 mL	0.7238 mL	1.4476 mL	3.6191 mL
	20 mM	0.1086 mL	0.5429 mL	1.0857 mL	2.7143 mL
	25 mM	0.0869 mL	0.4343 mL	0.8686 mL	2.1715 mL
	30 mM	0.0724 mL	0.3619 mL	0.7238 mL	1.8095 mL
	40 mM	0.0543 mL	0.2714 mL	0.5429 mL	1.3572 mL
	50 mM	0.0434 mL	0.2171 mL	0.4343 mL	1.0857 mL
	60 mM	0.0362 mL	0.1810 mL	0.3619 mL	0.9048 mL
	80 mM	0.0271 mL	0.1357 mL	0.2714 mL	0.6786 mL
	100 mM	0.0217 mL	0.1086 mL	0.2171 mL	0.5429 mL

TP0586532 Related Classifications

Infection Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TP05865322427584-96-9TP 0586532TP-0586532Bacterialnon-hydroxamatelowcardiovascularriskeffectivepneumoniaeresistantstrainsInhibitorinhibitorinhibit

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