Tropolone

Name: Tropolone
Brand: MedChemExpress
SKU: HY-N7135
Rating: 4.5 (1 reviews)

Cat. No.: HY-N7135 Purity: 99.93%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tropolone is a seven-membered non-benzenoid aromatic compound, which is the precursor of many Azulene derivatives. Tropolone is a potent mushroom tyrosinase inhibitor with an IC₅₀ value of 0.4 μM. Its inhibitory effect can be achieved by dialysis or excess CU²⁺ Reversa. Tropolone exhibits broad anti-viral and anti-fungal activity and is synergistic upon co-treatment with nucleos(t)ide analog drugs. Tropolone is a promising candidate for research in osteosarcoma.

For research use only. We do not sell to patients.

Tropolone Chemical Structure

CAS No. : 533-75-5

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Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
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1 g	USD 30	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

IC₅₀ & Target

IC50: 0.4 μM (mushroom tyrosinase)^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
HT-22	EC₅₀	> 10 μM Compound: tropolone	Neuroprotective activity against glutamate-mediated oxidative stress-induced cell death in mouse HT22 cells assessed as survival after 24 hrs by MTT assay Neuroprotective activity against glutamate-mediated oxidative stress-induced cell death in mouse HT22 cells assessed as survival after 24 hrs by MTT assay	[PMID: 20045220]

In Vitro

Tropolone (10 μM, 72 h) perfoerms differences in toxicity between HEK293 cells and HepDES19 cells, also exerts different sensibility in HOS with an IC₅₀ value of 0.67 μM and in SaOS-2 with an IC₅₀ value of 5.93 μM ^[1]^[2].
Tropolone (0-10 μM, 24 h) disrupts osteosarcoma cell migration, invasion and several key metabolic pathways by altering purine and pyrimidine metabolism in a variety of human osteosarcoma cell lines^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	HEK293 and HepDES19 cells
Concentration:	10 μM
Incubation Time:	72 h
Result:	Was 10-fold more toxic in the kidney cell line than the liver cell line.

Apoptosis Analysis^[2]

Cell Line:	143B, CAL-72, HOS, MG-63, SaOS-2 cells
Concentration:	0-10 μM
Incubation Time:	48-72 h
Result:	Induced apoptotic cell death in a variety of human osteosarcoma cell lines.

Molecular Weight

122.12

Formula

C₇H₆O₂

CAS No.

533-75-5

Appearance

Solid

Color

Off-white to light brown

SMILES

O=C1C(O)=CC=CC=C1

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (818.87 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (204.72 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.1887 mL	40.9433 mL	81.8867 mL
5 mM	1.6377 mL	8.1887 mL	16.3773 mL
10 mM	0.8189 mL	4.0943 mL	8.1887 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 20 mg/mL (163.77 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (280 KB) SDS (758 KB)

COA (255 KB) HNMR (120 KB) RP-HPLC (277 KB)

Handling Instructions (2659 KB)

References

[1]. Woodson ME, et al. In vitro evaluation of tropolone absorption, metabolism, and clearance. Antiviral Res. 2023 Dec;220:105762. [Content Brief]

[2]. Haney SL, et al. Investigation of the activity of a novel tropolone in osteosarcoma. Drug Dev Res. 2024 Feb;85(1):e22129. [Content Brief]

[3]. Donald D Nolting,et al. Synthesis of bicyclo[5.3.0]azulene derivatives. Nat Protoc. 2009; 4(8): 1113–1117. [Content Brief]

[4]. VardaKahn, et al.Inhibition of mushroom tyrosinase by tropolone Phytochemistry. Volume 24, Issue 5, 1985, Pages 905-908

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.1887 mL	40.9433 mL	81.8867 mL	204.7167 mL
	5 mM	1.6377 mL	8.1887 mL	16.3773 mL	40.9433 mL
	10 mM	0.8189 mL	4.0943 mL	8.1887 mL	20.4717 mL
	15 mM	0.5459 mL	2.7296 mL	5.4591 mL	13.6478 mL
	20 mM	0.4094 mL	2.0472 mL	4.0943 mL	10.2358 mL
	25 mM	0.3275 mL	1.6377 mL	3.2755 mL	8.1887 mL
	30 mM	0.2730 mL	1.3648 mL	2.7296 mL	6.8239 mL
	40 mM	0.2047 mL	1.0236 mL	2.0472 mL	5.1179 mL
	50 mM	0.1638 mL	0.8189 mL	1.6377 mL	4.0943 mL
	60 mM	0.1365 mL	0.6824 mL	1.3648 mL	3.4119 mL
	80 mM	0.1024 mL	0.5118 mL	1.0236 mL	2.5590 mL
	100 mM	0.0819 mL	0.4094 mL	0.8189 mL	2.0472 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Tropolone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tropolone533-75-5TyrosinaseFungalAntiproliferativeAnti-viralAnti-fungalTyrosinase InhibitorOsteosarcomaInhibitorinhibitorinhibit

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Tropolone

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Complete Stock Solution Preparation Table

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