2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK MAPK/ERK Pathway GPCR/G Protein Metabolic Enzyme/Protease
  3. EGFR Ras Phosphatase
  4. Tyrphostin 8

Tyrphostin 8 is a tyrosine kinase, with an IC50 of 560 μM for EGFR kinase. Tyrphostin 8 is also a GTPase inhibitor. Tyrphostin 8 can inhibit the protein serine/threonine phosphatase calcineurin (IC50=21 μM).

For research use only. We do not sell to patients.

Tyrphostin 8 Chemical Structure

Tyrphostin 8 Chemical Structure

CAS No. : 3785-90-8

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Based on 1 publication(s) in Google Scholar

Tyrphostin 8 Related Antibodies

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Description

Tyrphostin 8 is a tyrosine kinase, with an IC50 of 560 μM for EGFR kinase. Tyrphostin 8 is also a GTPase inhibitor. Tyrphostin 8 can inhibit the protein serine/threonine phosphatase calcineurin (IC50=21 μM)[1][2][3].

IC50 & Target[1][3]

EGFR

560 μM (IC50)

calcineuin phosphatase

21 μM (IC50)

In Vitro

Tyrphostin 8 (10-100 μM; pretreated for 20 min) blocks the Carbachol-initiated PKCδ tyrosine phosphorylation and ERK1/2 activation in parotid acinar cells[1].
Tyrphostin 8 (10-100 μM) produces a rapid and large increase in the basal O2 consumption of parotid acinar[1].
Tyrphostin 8 (10-100 μM) reduces the parotid ATP content by ∼90% at the concentration of 100 μM[1].
Tyrphostin 8 increases apical-to-basolateral transport of insulin-transferrin conjugate by enhancing transferrin receptor-mediated transcytosis in filter-grown Caco-2 cell monolayer[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tyrphostin 8 Related Antibodies

Western Blot Analysis[1]

Cell Line: Parotid acinar cells
Concentration: 10, 100 μM
Incubation Time: Pretreated for 20 min
Result: Reduced the increase in tyrosine phosphorylation of PKCδ initiated by carbachol.
Reduced the activation of ERK1/2 by carbachol.
In Vivo

Tyrphostin 8 improves the glucose-lowering effect of Insulin-transferrin in Streptozotocin-induced diabetic rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

170.17

Formula

C10H6N2O
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

C1=C(C=CC(=C1)O)C=C(C#N)C#N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (587.65 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.8765 mL 29.3824 mL 58.7648 mL
5 mM 1.1753 mL 5.8765 mL 11.7530 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

SDS (480 KB)

COA (245 KB) HNMR (106 KB) LCMS (239 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.8765 mL 29.3824 mL 58.7648 mL 146.9119 mL
5 mM 1.1753 mL 5.8765 mL 11.7530 mL 29.3824 mL
10 mM 0.5876 mL 2.9382 mL 5.8765 mL 14.6912 mL
15 mM 0.3918 mL 1.9588 mL 3.9177 mL 9.7941 mL
20 mM 0.2938 mL 1.4691 mL 2.9382 mL 7.3456 mL
25 mM 0.2351 mL 1.1753 mL 2.3506 mL 5.8765 mL
30 mM 0.1959 mL 0.9794 mL 1.9588 mL 4.8971 mL
40 mM 0.1469 mL 0.7346 mL 1.4691 mL 3.6728 mL
50 mM 0.1175 mL 0.5876 mL 1.1753 mL 2.9382 mL
60 mM 0.0979 mL 0.4897 mL 0.9794 mL 2.4485 mL
80 mM 0.0735 mL 0.3673 mL 0.7346 mL 1.8364 mL
100 mM 0.0588 mL 0.2938 mL 0.5876 mL 1.4691 mL
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Tyrphostin 8 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tyrphostin 83785-90-8Tyrphostin8Tyrphostin-8EGFRRasPhosphataseEpidermal growth factor receptorErbB-1HER1tyrosineGTPaseserinethreoninecalcineurinInhibitorinhibitorinhibit

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