UNC9994 hydrochloride

Name: UNC9994 hydrochloride
Brand: MedChemExpress
SKU: HY-111385
Rating: 4.5 (1 reviews)

Cat. No.: HY-111385 Purity: 98.83%: FAQs
Data Sheet SDS COA Handling Instructions

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UNC9994 hydrochloride is a functionally selective, β-arrestin–biased dopamine D₂ receptor (D₂R) agonist that selectively activates β-arrestin recruitment and signaling. UNC9994 hydrochloride shows a binding affinity with a K_i of 79 nM for D₂R. UNC9994 hydrochloride is also an antagonist of G_i-regulated cAMP production and partial agonist for D2R/β-arrestin-2 interactions. UNC9994 hydrochloride shows antipsychotic-like activity.

For research use only. We do not sell to patients.

UNC9994 hydrochloride Chemical Structure

CAS No. : 2108826-33-9

Size	Price	Stock	Quantity
5 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 375	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 820	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1450	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2500	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of UNC9994 hydrochloride:

UNC9994 Get quote

1 Publications Citing Use of MCE UNC9994 hydrochloride

•Theranostics. 2024 Jan 12;14(3):1126-1146. [Abstract]

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View All Dopamine Receptor Isoform Specific Products:

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Dopamine Receptor D₁ Receptor D₂ Receptor D₃ Receptor D₄ Receptor D₅ Receptor

View All 5-HT Receptor Isoform Specific Products:

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5-HT₁ Receptor 5-HT₂ Receptor 5-HT₃ Receptor 5-HT₄ Receptor 5-HT₅ Receptor 5-HT₆ Receptor 5-HT₇ Receptor 5-HT Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

D₃ Receptor

17 nM (Ki)

D₂ Receptor

79 nM (Ki)

D₄ Receptor

138 nM (Ki)

D₁ Receptor

4000 nM (Ki)

5-HT_2B Receptor

25 nM (Ki)

5-HT_1A Receptor

26 nM (Ki)

5-HT_2A Receptor

140 nM (Ki)

5-HT_2C Receptor

512 nM (Ki)

In Vitro

UNC9994 hydrochloride induces D₂-mediated β-arrestin-2 translocation with an EC₅₀s of 6.1 nM and 448 nM in Tango assay and DiscoveRx assay, respectively^[1].
UNC9994 hydrochloride is an antagonist at 5HT_2A and 5HT_2B and an agonist at 5HT_2C and 5HT_1A^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

UNC9994 (2.0 mg/kg; i.p.; once) hydrochloride shows antipsychotic activity that is attenuated in β-arrestin-2 knockout mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J wild-type and β-arrestin-2 knockout mice^[1]
Dosage:	2.0 mg/kg, followed 30 min later with 6 mg/kg phencyclidine (PCP, i.p.)
Administration:	IP, once
Result:	Markedly inhibited PCP-induced hyperlocomotion in wild-type mice and the activity was completely abolished in β-arrestin-2 knockout mice.

Molecular Weight

457.84

Formula

C₂₁H₂₃Cl₃N₂OS

CAS No.

2108826-33-9

Appearance

Solid

Color

White to off-white

SMILES

ClC1=C(Cl)C(C2CCN(CCCOC3=CC=C(SC=N4)C4=C3)CC2)=CC=C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (218.42 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1842 mL	10.9208 mL	21.8417 mL
5 mM	0.4368 mL	2.1842 mL	4.3683 mL
10 mM	0.2184 mL	1.0921 mL	2.1842 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.46 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.46 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (5.46 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.83%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (247 KB) LCMS (298 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)

References

[1]. Allen JA, et al. Discovery of β-arrestin-biased dopamine D2 ligands for probing signal transduction pathways essential for antipsychotic efficacy. Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18488-93. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1842 mL	10.9208 mL	21.8417 mL	54.6042 mL
	5 mM	0.4368 mL	2.1842 mL	4.3683 mL	10.9208 mL
	10 mM	0.2184 mL	1.0921 mL	2.1842 mL	5.4604 mL
	15 mM	0.1456 mL	0.7281 mL	1.4561 mL	3.6403 mL
	20 mM	0.1092 mL	0.5460 mL	1.0921 mL	2.7302 mL
	25 mM	0.0874 mL	0.4368 mL	0.8737 mL	2.1842 mL
	30 mM	0.0728 mL	0.3640 mL	0.7281 mL	1.8201 mL
	40 mM	0.0546 mL	0.2730 mL	0.5460 mL	1.3651 mL
	50 mM	0.0437 mL	0.2184 mL	0.4368 mL	1.0921 mL
	60 mM	0.0364 mL	0.1820 mL	0.3640 mL	0.9101 mL
	80 mM	0.0273 mL	0.1365 mL	0.2730 mL	0.6826 mL
	100 mM	0.0218 mL	0.1092 mL	0.2184 mL	0.5460 mL