2. Membrane Transporter/Ion Channel Neuronal Signaling Metabolic Enzyme/Protease Immunology/Inflammation NF-κB Apoptosis
  3. Calcium Channel Reactive Oxygen Species Apoptosis Endogenous Metabolite
  4. Urolithin C

Urolithin C, a gut-microbial metabolite of Ellagic acid, is a glucose-dependent activator of insulin secretion. Urolithin C is a L-type Ca2+ channel opener and enhances Ca2+ influx. Urolithin C induces cell apoptosis through a mitochondria-mediated pathway and also stimulates reactive oxygen species (ROS) formation.

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Urolithin C Chemical Structure

Urolithin C Chemical Structure

CAS No. : 165393-06-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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50 mg USD 300 In-stock
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Based on 4 publication(s) in Google Scholar

Urolithin C Related Antibodies

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

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Description

Urolithin C, a gut-microbial metabolite of Ellagic acid, is a glucose-dependent activator of insulin secretion. Urolithin C is a L-type Ca2+ channel opener and enhances Ca2+ influx. Urolithin C induces cell apoptosis through a mitochondria-mediated pathway and also stimulates reactive oxygen species (ROS) formation[1][2].

IC50 & Target

L-type calcium channel

 

In Vitro

Urolithin C (20-100 μM; 1 hour; INS-1 cells) treatment enhances glucose-induced extracellular signal-regulated kinases 1/2 (ERK1/2) activation in INS-1 β-cells[1].
Urolithin C significantly inhibits the proliferation of PC12 cells. Urolithin C treatment actively increases the lactate dehydrogenase (LDH) release and lipid peroxidation malondialdehyde (MDA), stimulates reactive oxygen species (ROS) formation and mitochondrial membrane depolarization, and caused calcium dyshomeostasis[2].
Urolithin C treatment induces apoptosis and S phase cell cycle arrest[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Urolithin C Related Antibodies

Western Blot Analysis[1]

Cell Line: INS-1 cells
Concentration: 20 μM, 100 μM
Incubation Time: 1 hour
Result: Enhanced glucose-induced extracellular signal-regulated kinases 1/2 (ERK1/2) activation.
In Vivo

The pharmacokinetics of Urolithin C (10 mg/kg; intraperitoneal administration) in male Wistar rat (140-160 g) are studied. The half-life of the terminal part is 11.3 h and the total clearance (CL/F) is 3.41 L/h/kg. The initial volume of distribution (V1/F) and the steady-state volume of distribution (Vss/F) are 0.831 L/kg and 55.6 L/kg, respectively[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

244.20

Formula

C13H8O5
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

O=C1C2=CC(O)=C(O)C=C2C3=CC=C(O)C=C3O1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (204.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0950 mL 20.4750 mL 40.9500 mL
5 mM 0.8190 mL 4.0950 mL 8.1900 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (8.52 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (8.52 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (250 KB) HNMR (269 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.0950 mL 20.4750 mL 40.9500 mL 102.3751 mL
5 mM 0.8190 mL 4.0950 mL 8.1900 mL 20.4750 mL
10 mM 0.4095 mL 2.0475 mL 4.0950 mL 10.2375 mL
15 mM 0.2730 mL 1.3650 mL 2.7300 mL 6.8250 mL
20 mM 0.2048 mL 1.0238 mL 2.0475 mL 5.1188 mL
25 mM 0.1638 mL 0.8190 mL 1.6380 mL 4.0950 mL
30 mM 0.1365 mL 0.6825 mL 1.3650 mL 3.4125 mL
40 mM 0.1024 mL 0.5119 mL 1.0238 mL 2.5594 mL
50 mM 0.0819 mL 0.4095 mL 0.8190 mL 2.0475 mL
60 mM 0.0683 mL 0.3413 mL 0.6825 mL 1.7063 mL
80 mM 0.0512 mL 0.2559 mL 0.5119 mL 1.2797 mL
100 mM 0.0410 mL 0.2048 mL 0.4095 mL 1.0238 mL
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Urolithin C Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Urolithin C165393-06-6Calcium ChannelReactive Oxygen SpeciesApoptosisEndogenous MetaboliteCa2+ channelsCa channelsPolyphenolsgut-microbialmetaboliteβ-cellsglucose-dependentinsulinERK1/2ROSmitochondriaanti-diabeticInhibitorinhibitorinhibit

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