Usaramine

Name: Usaramine
Brand: MedChemExpress
SKU: HY-N6931
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6931 Purity: 99.57%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Usaramine is a pyrrolizidine alkaloid isolated from seeds of Crolatalaria pallida. Usaramine demonstrates a highlighted antibiofilm activity against Staphylococcus epidermidis by reducing more than 50% of biofilm formation without killing the bacteria.

For research use only. We do not sell to patients.

Usaramine Chemical Structure

CAS No. : 15503-87-4

Size	Price	Stock	Quantity
5 mg	USD 125	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Usaramine:

Usaramine (Standard) Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A204	IC₅₀	120 μg/mL Compound: 3	Cytotoxicity against human A204 cells after 24 hrs by soft agar colony forming assay Cytotoxicity against human A204 cells after 24 hrs by soft agar colony forming assay	[PMID: 3210016]
CHO	EC₅₀	> 100 μg/mL Compound: 8	Toxicity against Cricetulus griseus CHO (Chinese hamster ovary) cell by MTT assay Toxicity against Cricetulus griseus CHO (Chinese hamster ovary) cell by MTT assay	10.1016/jbse.2007.08.015
CHO	EC₅₀	> 100 μg/mL Compound: 7	Toxicity against Cricetulus griseus CHO (Chinese hamster ovary) cell by MTT assay Toxicity against Cricetulus griseus CHO (Chinese hamster ovary) cell by MTT assay	10.1016/jbse.2007.08.015
Sf9	EC₅₀	> 100 μg/mL Compound: 8	Toxicity against Spodoptera frugiperda (fall armyworm) Sf9 cells by MTT assay Toxicity against Spodoptera frugiperda (fall armyworm) Sf9 cells by MTT assay	10.1016/jbse.2007.08.015
Sf9	EC₅₀	> 100 μg/mL Compound: 7	Toxicity against Spodoptera frugiperda (fall armyworm) Sf9 cells by MTT assay Toxicity against Spodoptera frugiperda (fall armyworm) Sf9 cells by MTT assay	10.1016/jbse.2007.08.015

Molecular Weight

351.39

Formula

C₁₈H₂₅NO₆

CAS No.

15503-87-4

Appearance

Solid

Color

White to off-white

SMILES

O=C1O[C@@]2([H])[C@@]3([H])C(COC([C@](CO)(O)[C@H](C)C/C1=C\C)=O)=CCN3CC2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (284.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8458 mL	14.2292 mL	28.4584 mL
5 mM	0.5692 mL	2.8458 mL	5.6917 mL
10 mM	0.2846 mL	1.4229 mL	2.8458 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.57%

Select Batch:

Data Sheet (279 KB) SDS (251 KB)

COA (259 KB) HNMR (277 KB) LCMS (256 KB)

Handling Instructions (2659 KB)

References

[1]. da Silva Negreiros Neto T, et al. Activity of pyrrolizidine alkaloids against biofilm formation and Trichomonas vaginalis. Biomed Pharmacother. 2016 Oct;83:323-329. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8458 mL	14.2292 mL	28.4584 mL	71.1460 mL
	5 mM	0.5692 mL	2.8458 mL	5.6917 mL	14.2292 mL
	10 mM	0.2846 mL	1.4229 mL	2.8458 mL	7.1146 mL
	15 mM	0.1897 mL	0.9486 mL	1.8972 mL	4.7431 mL
	20 mM	0.1423 mL	0.7115 mL	1.4229 mL	3.5573 mL
	25 mM	0.1138 mL	0.5692 mL	1.1383 mL	2.8458 mL
	30 mM	0.0949 mL	0.4743 mL	0.9486 mL	2.3715 mL
	40 mM	0.0711 mL	0.3557 mL	0.7115 mL	1.7787 mL
	50 mM	0.0569 mL	0.2846 mL	0.5692 mL	1.4229 mL
	60 mM	0.0474 mL	0.2372 mL	0.4743 mL	1.1858 mL
	80 mM	0.0356 mL	0.1779 mL	0.3557 mL	0.8893 mL
	100 mM	0.0285 mL	0.1423 mL	0.2846 mL	0.7115 mL

Usaramine Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Usaramine15503-87-4BacterialpyrrolizidinealkaloidbiofilmbacteriaInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Usaramine

Customer Review

Other Forms of Usaramine:

Usaramine Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Usaramine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report