2. Apoptosis
  3. Caspase
  4. Z-DEVD-AFC

Z-DEVD-AFC is a cell-permeant substrate for caspase-3, which causes a shift in fluorescence uponcleavage of the AFC fluorophore. Z-DEVD-AFC can be used to detect caspase-3-like enzymes activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Z-DEVD-AFC Chemical Structure

Z-DEVD-AFC Chemical Structure

CAS No. : 1135416-11-3

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5 mg USD 320 In-stock
10 mg USD 512 In-stock
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Based on 1 publication(s) in Google Scholar

Z-DEVD-AFC Related Antibodies

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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Description

Z-DEVD-AFC is a cell-permeant substrate for caspase-3, which causes a shift in fluorescence uponcleavage of the AFC fluorophore. Z-DEVD-AFC can be used to detect caspase-3-like enzymes activity[1].

Molecular Weight

821.71

Formula

C36H38F3N5O14
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

500
Excitation (Ex)

380
Sequence Shortening

zDEVD-AFC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (60.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2170 mL 6.0849 mL 12.1697 mL
5 mM 0.2434 mL 1.2170 mL 2.4339 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2170 mL 6.0849 mL 12.1697 mL 30.4244 mL
5 mM 0.2434 mL 1.2170 mL 2.4339 mL 6.0849 mL
10 mM 0.1217 mL 0.6085 mL 1.2170 mL 3.0424 mL
15 mM 0.0811 mL 0.4057 mL 0.8113 mL 2.0283 mL
20 mM 0.0608 mL 0.3042 mL 0.6085 mL 1.5212 mL
25 mM 0.0487 mL 0.2434 mL 0.4868 mL 1.2170 mL
30 mM 0.0406 mL 0.2028 mL 0.4057 mL 1.0141 mL
40 mM 0.0304 mL 0.1521 mL 0.3042 mL 0.7606 mL
50 mM 0.0243 mL 0.1217 mL 0.2434 mL 0.6085 mL
60 mM 0.0203 mL 0.1014 mL 0.2028 mL 0.5071 mL
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Z-DEVD-AFC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Z-DEVD-AFC1135416-11-3Caspasefluorogenic caspace-3 substratefluorescence of AFCpeptideInhibitorinhibitorinhibit

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