2. Metabolic Enzyme/Protease
  3. FXR
  4. ZLY28

ZLY28 is the first-in-class intestinal restricted and orally active FXR and FABP1 dual modulator. ZLY28 also is a novel anti-NASH agent. ZLY28 can be used for the research of nonalcoholic steatohepatitis (NASH).

For research use only. We do not sell to patients.

ZLY28 Chemical Structure

ZLY28 Chemical Structure

CAS No. : 3004676-77-8

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5 mg USD 300 In-stock
10 mg USD 480 In-stock
25 mg USD 1000 In-stock
50 mg USD 1600 In-stock
100 mg USD 2600 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

ZLY28 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

ZLY28 is the first-in-class intestinal restricted and orally active FXR and FABP1 dual modulator. ZLY28 also is a novel anti-NASH agent. ZLY28 can be used for the research of nonalcoholic steatohepatitis (NASH)[1].
IC50 & Target

EC50: 143 nM (FXR)[1].
IC50: 2.7 μM (FABP1)[1].
In Vitro

ZLY28 has suitable stability of liver microsomes and high target selectivity for FXR (EC50 = 143 nM) and FABP1 (IC50 = 2.7 μM)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ZLY28 Related Antibodies
In Vivo

ZLY28 (oral; 20 mg/kg) significantly alleviates fatty liver by regulating multiple pathogeneses, including lipid metabolism, inflammation, oxidative stress, and fibrosis in the NASH mice model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 8 weeks old male C57BL/6 mice[1]
Dosage: 20 mg/kg
Administration: Oral administration
Result: Mainly distributed in the ileum with an ileum/plasma ratio of 104.1.
Significantly alleviated hepatic steatosis, lobular inflammation and ballooning.
Improved hepatic lipid homeostasis by inhibiting lipogenesis and promoting lipolysis.
Downregulated the gene expression levels.
Exhibited an acceptable safety profile with no acute toxicity.
Molecular Weight

520.40

Formula

C29H23Cl2NO4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1(C2=CC(C3=CC=C(OCC4=C(C5CC5)ON=C4C6=C(Cl)C=CC=C6Cl)C=C3)=CC=C2)CC1)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (192.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9216 mL 9.6080 mL 19.2160 mL
5 mM 0.3843 mL 1.9216 mL 3.8432 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (9.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (9.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9216 mL 9.6080 mL 19.2160 mL 48.0400 mL
5 mM 0.3843 mL 1.9216 mL 3.8432 mL 9.6080 mL
10 mM 0.1922 mL 0.9608 mL 1.9216 mL 4.8040 mL
15 mM 0.1281 mL 0.6405 mL 1.2811 mL 3.2027 mL
20 mM 0.0961 mL 0.4804 mL 0.9608 mL 2.4020 mL
25 mM 0.0769 mL 0.3843 mL 0.7686 mL 1.9216 mL
30 mM 0.0641 mL 0.3203 mL 0.6405 mL 1.6013 mL
40 mM 0.0480 mL 0.2402 mL 0.4804 mL 1.2010 mL
50 mM 0.0384 mL 0.1922 mL 0.3843 mL 0.9608 mL
60 mM 0.0320 mL 0.1601 mL 0.3203 mL 0.8007 mL
80 mM 0.0240 mL 0.1201 mL 0.2402 mL 0.6005 mL
100 mM 0.0192 mL 0.0961 mL 0.1922 mL 0.4804 mL
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ZLY28 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ZLY283004676-77-8ZLY 28ZLY-28FXRNR1H4first-in-classFABP1anti-NASHnonalcoholic steatohepatitis (NASH)Inhibitorinhibitorinhibit

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