ZM 306416 (Synonyms: CB 676475)

Name: ZM 306416
Brand: MedChemExpress
SKU: HY-13785
Rating: 5.0 (2 reviews)

Cat. No.: HY-13785 Purity: 99.80%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ZM-306416 (CB 676475) is a potent inhibitor of VEGFR with IC₅₀s of 0.1 and 2 μM for KDR and Flt, respectively. ZM-306416 is also a EGFR inhibitor with an IC₅₀ of <10 nM.

For research use only. We do not sell to patients.

ZM 306416 Chemical Structure

CAS No. : 690206-97-4

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 50	Get quote	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 270	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 430	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of ZM 306416:

ZM-306416 hydrochloride Get quote

2 Publications Citing Use of MCE ZM 306416

Featured Recommendations

•Related Screening Libraries:

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View All VEGFR Isoform Specific Products:

View All Isoforms

VEGFR1/Flt-1 VEGFR2/KDR/Flk-1 VEGFR3/Flt-4

Biological Activity
Purity & Documentation
References
Customer Review

Description

ZM-306416 (CB 676475) is a potent inhibitor of VEGFR with IC₅₀s of 0.1 and 2 μM for KDR and Flt, respectively. ZM-306416 is also a EGFR inhibitor with an IC₅₀ of <10 nM.

IC₅₀ & Target^[1]

KDR

100 nM (IC₅₀)

Flt-1

2 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A498	IC₅₀	14.1 μM Compound: 19, CB676475	Inhibition of VEGFR1 tyrosine kinase activity in VEGF-stimulated human A498 cells after 60 mins by ELISA Inhibition of VEGFR1 tyrosine kinase activity in VEGF-stimulated human A498 cells after 60 mins by ELISA	[PMID: 22204741]
A498	IC₅₀	14.1 μM Compound: 48, CB-676475	Inhibition of VEGFR1 phosphorylation in human A498 cells after 10 mins by FLISA Inhibition of VEGFR1 phosphorylation in human A498 cells after 10 mins by FLISA	[PMID: 20403700]
A498	IC₅₀	14.1 μM Compound: 26, CB676475	Inhibition of VEGF-induced VEGFR1 phosphorylation in human A498 cells overexpressing Flt-1 pretreated for 60 mins prior to VEGF addition measured after 10 mins by phosphotyrosine ELISA cytoblot analysis Inhibition of VEGF-induced VEGFR1 phosphorylation in human A498 cells overexpressing Flt-1 pretreated for 60 mins prior to VEGF addition measured after 10 mins by phosphotyrosine ELISA cytoblot analysis	[PMID: 23375090]
A498	IC₅₀	14.1 μM Compound: 14, CB-676475	Inhibition of VEGFR1 in human A498 cells by phosphotyrosine cell-based ELISA Inhibition of VEGFR1 in human A498 cells by phosphotyrosine cell-based ELISA	[PMID: 20092323]
A498	IC₅₀	17.7 μM Compound: 21, CB-676475	Inhibition of VEGF-induced VEGFR1 autophosphorylation in human A498 cells incubated for 60 mins prior to VEGF-induction measured after 10 mins by phosphotyrosine ELISA Inhibition of VEGF-induced VEGFR1 autophosphorylation in human A498 cells incubated for 60 mins prior to VEGF-induction measured after 10 mins by phosphotyrosine ELISA	[PMID: 22370340]

In Vitro

ZM-306416 selective anti-proliferative effect toward the EGFR addicted NSCLC cell lines H3255 and HCC4011 (IC₅₀=0.09±0.007 μM and 0.072±0.001 μM respectively), while sparing the wild type EGFR cell lines A549 and H2030 (IC₅₀>10 μM). ZM-306416 is also found to inhibit the ABL in vitro kinase activity with a less potent IC₅₀ value of 1.3±0.2 μM toward the ABL kinase^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

333.74

Formula

C₁₆H₁₃ClFN₃O₂

CAS No.

690206-97-4

Appearance

Solid

Color

White to gray

SMILES

COC1=CC2=NC=NC(NC3=C(F)C=C(Cl)C=C3)=C2C=C1OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (149.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9963 mL	14.9817 mL	29.9634 mL
5 mM	0.5993 mL	2.9963 mL	5.9927 mL
10 mM	0.2996 mL	1.4982 mL	2.9963 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (251 KB) HNMR (167 KB) RP-HPLC (335 KB)

Handling Instructions (2659 KB)

References

[1]. Han SY, Park SS, Lee WG, Synthesis of a novel biotin-tagged photoaffinity probe for VEGF receptor tyrosine kinases. Bioorg Med Chem Lett. 2006 Jan 1;16(1):129-33. [Content Brief]

[2]. Antczak C, Mahida JP, Bhinder B, A high-content biosensor-based screen identifies cell-permeable activators and inhibitors of EGFR function: implications in drug discovery. J Biomol Screen. 2012 Aug;17(7):885-99. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9963 mL	14.9817 mL	29.9634 mL	74.9086 mL
	5 mM	0.5993 mL	2.9963 mL	5.9927 mL	14.9817 mL
	10 mM	0.2996 mL	1.4982 mL	2.9963 mL	7.4909 mL
	15 mM	0.1998 mL	0.9988 mL	1.9976 mL	4.9939 mL
	20 mM	0.1498 mL	0.7491 mL	1.4982 mL	3.7454 mL
	25 mM	0.1199 mL	0.5993 mL	1.1985 mL	2.9963 mL
	30 mM	0.0999 mL	0.4994 mL	0.9988 mL	2.4970 mL
	40 mM	0.0749 mL	0.3745 mL	0.7491 mL	1.8727 mL
	50 mM	0.0599 mL	0.2996 mL	0.5993 mL	1.4982 mL
	60 mM	0.0499 mL	0.2497 mL	0.4994 mL	1.2485 mL
	80 mM	0.0375 mL	0.1873 mL	0.3745 mL	0.9364 mL
	100 mM	0.0300 mL	0.1498 mL	0.2996 mL	0.7491 mL

ZM 306416 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ZM 306416690206-97-4CB 676475ZM306416ZM-306416CB676475CB-676475VEGFRVascular endothelial growth factor receptorInhibitorinhibitorinhibit

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ZM 306416 (Synonyms: CB 676475)

Customer Review

Other Forms of ZM 306416:

ZM 306416 Related Antibodies

2 Publications Citing Use of MCE ZM 306416

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All VEGFR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

ZM 306416 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ZM 306416 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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