2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. 2-Fluoroadenine

2-Fluoroadenine is a toxic purine bases. 2-Fluoroadenine has toxicity in nonproliferating and proliferating tumor cells. 2-Fluoroadenine can be used for researching anticancer.

For research use only. We do not sell to patients.

2-Fluoroadenine Chemical Structure

2-Fluoroadenine Chemical Structure

CAS No. : 700-49-2

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Based on 1 publication(s) in Google Scholar

2-Fluoroadenine Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

2-Fluoroadenine is a toxic purine bases. 2-Fluoroadenine has toxicity in nonproliferating and proliferating tumor cells. 2-Fluoroadenine can be used for researching anticancer[1].

IC50 & Target

DNA/RNA synthesis[1]
In Vitro

2-Fluoroadenine (2 μM; 4 hours) inhibits CEM cell growth by targeting one or more enzymes involved in either RNA or protein synthesis[1].
2-Fluoroadenine (0-1000 μM; 96 hours) exhibits cytotoxicity in nonproliferating MRC-5 cells[1].
2-Fluoroadenine (0.22, 2.2 and 22 μM; 30 hours) inhibits protein, RNA, and DNA syntheses in Balb-3T3 cells incubated in serum-free medium[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-Fluoroadenine Related Antibodies

Cell Proliferation Assay

Cell Line: CEM cells[1]
Concentration: 2 μM
Incubation Time: 4 hours
Result: Inhibited CEM cell growth by targeting one or more enzymes involved in either RNA or protein synthesis.

Cell Cytotoxicity Assay

Cell Line: MRC-5 cells[1]
Concentration: 0-1000 μM
Incubation Time: 4 hours
Result: Exhibited cytotoxicity in nonproliferating MRC-5 cells.
Molecular Weight

153.12

Formula

C5H4FN5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=NC(F)=NC2=C1NC=N2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 20.83 mg/mL (136.04 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5308 mL 32.6541 mL 65.3083 mL
5 mM 1.3062 mL 6.5308 mL 13.0617 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (13.58 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (13.58 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.56%

COA (252 KB) HNMR (250 KB) LCMS (88 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.5308 mL 32.6541 mL 65.3083 mL 163.2706 mL
5 mM 1.3062 mL 6.5308 mL 13.0617 mL 32.6541 mL
10 mM 0.6531 mL 3.2654 mL 6.5308 mL 16.3271 mL
15 mM 0.4354 mL 2.1769 mL 4.3539 mL 10.8847 mL
20 mM 0.3265 mL 1.6327 mL 3.2654 mL 8.1635 mL
25 mM 0.2612 mL 1.3062 mL 2.6123 mL 6.5308 mL
30 mM 0.2177 mL 1.0885 mL 2.1769 mL 5.4424 mL
40 mM 0.1633 mL 0.8164 mL 1.6327 mL 4.0818 mL
50 mM 0.1306 mL 0.6531 mL 1.3062 mL 3.2654 mL
60 mM 0.1088 mL 0.5442 mL 1.0885 mL 2.7212 mL
80 mM 0.0816 mL 0.4082 mL 0.8164 mL 2.0409 mL
100 mM 0.0653 mL 0.3265 mL 0.6531 mL 1.6327 mL
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2-Fluoroadenine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Fluoroadenine700-49-2DNA/RNA SynthesisAnticancerPurine basesCEMMRC-5CytotoxicityInhibitorinhibitorinhibit

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