1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. 4,6-Dioxoheptanoic acid

4,6-Dioxoheptanoic acid is a potent inhibitor of heme biosynthesis.

For research use only. We do not sell to patients.

4,6-Dioxoheptanoic acid Chemical Structure

4,6-Dioxoheptanoic acid Chemical Structure

CAS No. : 51568-18-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 5 publication(s) in Google Scholar

Other Forms of 4,6-Dioxoheptanoic acid:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

4,6-Dioxoheptanoic acid is a potent inhibitor of heme biosynthesis.

IC50 & Target

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
Sf9 IC50
12.7 μM
Compound: 28
Inhibition of human KDM3A catalytic domain expressed in Sf9 cells methyl lysine peptide substrate by AlphaScreen assay
Inhibition of human KDM3A catalytic domain expressed in Sf9 cells methyl lysine peptide substrate by AlphaScreen assay
[PMID: 22724510]
Sf9 IC50
4.5 μM
Compound: 28
Inhibition of human KDM5C catalytic domain expressed in Sf9 cells using methyl lysine peptide substrate by AlphaScreen assay
Inhibition of human KDM5C catalytic domain expressed in Sf9 cells using methyl lysine peptide substrate by AlphaScreen assay
[PMID: 22724510]
In Vitro

4,6-Dioxoheptanoic acid (Succinylacetone, SA) is examined with regard to its ability to a) inhibit the second enzyme of the heme pathway, δ-aminolevulinic acid (ALA) dehydratase, b) lower the heme concentration, and c) inhibit cell growth of murine erythroleukemia (MEL) cells in culture[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

158.15

Formula

C7H10O4

CAS No.
Unlabeled CAS

51568-18-4

Appearance

Solid

Color

White to off-white

SMILES

CC(=O)CC(=O)CCC(O)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (632.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (632.31 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.3231 mL 31.6156 mL 63.2311 mL
5 mM 1.2646 mL 6.3231 mL 12.6462 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (15.81 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (15.81 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 6.3231 mL 31.6156 mL 63.2311 mL 158.0778 mL
5 mM 1.2646 mL 6.3231 mL 12.6462 mL 31.6156 mL
10 mM 0.6323 mL 3.1616 mL 6.3231 mL 15.8078 mL
15 mM 0.4215 mL 2.1077 mL 4.2154 mL 10.5385 mL
20 mM 0.3162 mL 1.5808 mL 3.1616 mL 7.9039 mL
25 mM 0.2529 mL 1.2646 mL 2.5292 mL 6.3231 mL
30 mM 0.2108 mL 1.0539 mL 2.1077 mL 5.2693 mL
40 mM 0.1581 mL 0.7904 mL 1.5808 mL 3.9519 mL
50 mM 0.1265 mL 0.6323 mL 1.2646 mL 3.1616 mL
60 mM 0.1054 mL 0.5269 mL 1.0539 mL 2.6346 mL
80 mM 0.0790 mL 0.3952 mL 0.7904 mL 1.9760 mL
100 mM 0.0632 mL 0.3162 mL 0.6323 mL 1.5808 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Product Name:
4,6-Dioxoheptanoic acid
Cat. No.:
HY-W010184
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