4,6-O-Ethylidene-α-D-glucose (Synonyms: Ethylidene-glucose)

Name: 4,6-O-Ethylidene-α-D-glucose
Brand: MedChemExpress
SKU: HY-N7433
Rating: 4.5 (1 reviews)

Cat. No.: HY-N7433 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4,6-O-ethylidene-α-D-glucose (Ethylidene-glucose), a glucose derivative, is a competitive exofacial binding-site inhibitor on glucose transporter 1 (GLUT1) with a K_i of 12 mM for wild-type 2-deoxy-D-glucose transport.

For research use only. We do not sell to patients.

4,6-O-Ethylidene-α-D-glucose Chemical Structure

CAS No. : 13224-99-2

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10 mM * 1 mL in DMSO ready for reconstitution	USD 110	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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References
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Description

IC₅₀ & Target^[1]

GLUT1

Human Endogenous Metabolite

In Vitro

4,6-O-ethylidene-α-D-glucose (Ethylidene-glucose) shows poor affinity for malarial hexose transporter (PfHT1; K_i>50 mM). 4,6-O-ethylidene-α-D-glucose inhibits wild-type transport with a K_i of approximately 12 mM, but this is increased to greater than 120 mM in the Gln282-Leu mutant^[1].
4,6-O-ethylidene-α-D-glucose inhibits glucose exit competitively but its penetration into human red cells is unaffected by glucose in the medium. The potentiation of the development of FDNB inhibition by sugars in the incubating medium is absent when 4,6-O-ethylidene-α-D-glucose is used and there is a slight protective action. 4,6-O-ethylidene-α-D-glucose penetrates human red cells by simple diffusion supported by its penetration of guinea-pig red cells at similar rates, by the occurrence of osmotic haemolysis in isosmotic solutions which is unaffected by copper ions and by the relatively high ether/water partition of the compound^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

206.19

Formula

C₈H₁₄O₆

CAS No.

13224-99-2

Appearance

Solid

Color

White to off-white

SMILES

[H][C@]12[C@@](O[C@H](O)[C@H](O)[C@H]2O)([H])COC(C)O1

Structure Classification

Saccharides

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (606.24 mM; Need ultrasonic)

DMSO : 100 mg/mL (484.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8499 mL	24.2495 mL	48.4990 mL
5 mM	0.9700 mL	4.8499 mL	9.6998 mL
10 mM	0.4850 mL	2.4249 mL	4.8499 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (484.99 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (278 KB) SDS (394 KB)

COA (253 KB) HNMR (245 KB)

Handling Instructions (2659 KB)

References

[1]. M Hashiramoto, et al. Site-directed Mutagenesis of GLUT1 in Helix 7 Residue 282 Results in Perturbation of Exofacial Ligand Binding. J Biol Chem. 1992 Sep 5;267(25):17502-7. [Content Brief]

[2]. Malay Patra, et al. A Potent Glucose-Platinum Conjugate Exploits Glucose Transporters and Preferentially Accumulates in Cancer Cells. Angew Chem Int Ed Engl. 2016 Feb 12;55(7):2550-4. [Content Brief]

[3]. G F Baker, et al. The Permeation of Human Red Cells by 4,6-O-ethylidene- -D-glucopyranose (Ethylidene Glucose). J Physiol. 1973 May;231(1):129-42. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.8499 mL	24.2495 mL	48.4990 mL	121.2474 mL
	5 mM	0.9700 mL	4.8499 mL	9.6998 mL	24.2495 mL
	10 mM	0.4850 mL	2.4249 mL	4.8499 mL	12.1247 mL
	15 mM	0.3233 mL	1.6166 mL	3.2333 mL	8.0832 mL
	20 mM	0.2425 mL	1.2125 mL	2.4249 mL	6.0624 mL
	25 mM	0.1940 mL	0.9700 mL	1.9400 mL	4.8499 mL
	30 mM	0.1617 mL	0.8083 mL	1.6166 mL	4.0416 mL
	40 mM	0.1212 mL	0.6062 mL	1.2125 mL	3.0312 mL
	50 mM	0.0970 mL	0.4850 mL	0.9700 mL	2.4249 mL
	60 mM	0.0808 mL	0.4042 mL	0.8083 mL	2.0208 mL
	80 mM	0.0606 mL	0.3031 mL	0.6062 mL	1.5156 mL
	100 mM	0.0485 mL	0.2425 mL	0.4850 mL	1.2125 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

4,6-O-Ethylidene-α-D-glucose Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4,6-O-Ethylidene-α-D-glucose13224-99-2Ethylidene-glucoseGLUTEndogenous MetaboliteGlucose transporterglucosederivativecompetitiveexofacialbinding-sitetransporter1GLUT12-deoxy-D-glucosetransportInhibitorinhibitorinhibit

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4,6-O-Ethylidene-α-D-glucose (Synonyms: Ethylidene-glucose)

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4,6-O-Ethylidene-α-D-glucose Related Antibodies

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All GLUT Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

4,6-O-Ethylidene-α-D-glucose Related Antibodies

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Complete Stock Solution Preparation Table

4,6-O-Ethylidene-α-D-glucose Related Classifications

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