4-Diethylaminobenzaldehyde

Name: 4-Diethylaminobenzaldehyde
Brand: MedChemExpress
SKU: HY-W016645
Rating: 4.5 (1 reviews)

Cat. No.: HY-W016645 Purity: 99.59%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-Diethylaminobenzaldehyde is a reversible aldehyde dehydrogenases (ALDHs) inhibitor, with a K_i of 4 nM for ALDH1. 4-Diethylaminobenzaldehyde displays potent anti-androgenic effect (IC₅₀= 1.71μM).

For research use only. We do not sell to patients.

4-Diethylaminobenzaldehyde Chemical Structure

CAS No. : 120-21-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 39	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 39	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE 4-Diethylaminobenzaldehyde

•Nat Commun. 2023 Sep 5;14(1):5398. [Abstract]

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•Related Screening Libraries:

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View All Aldehyde Dehydrogenase (ALDH) Isoform Specific Products:

View All Isoforms

ALDH1 ALDH2 ALDH3

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
DU-145	IC₅₀	> 200 μM Compound: DEAB	Antiproliferative activity against human DU145 cells expressing ALDH1A1 assessed as reduction in cell survival measured after 96 hrs by MTT assay Antiproliferative activity against human DU145 cells expressing ALDH1A1 assessed as reduction in cell survival measured after 96 hrs by MTT assay	[PMID: 35212533]
LNCaP	IC₅₀	> 200 μM Compound: DEAB	Antiproliferative activity against human LNCaP cells expressing ALDH1A3 assessed as reduction in cell survival measured after 96 hrs by MTT assay Antiproliferative activity against human LNCaP cells expressing ALDH1A3 assessed as reduction in cell survival measured after 96 hrs by MTT assay	[PMID: 35212533]
PC-3	IC₅₀	> 200 μM Compound: DEAB	Antiproliferative activity against human PC-3 cells expressing ALDH1A1 and ALDH3A1 assessed as reduction in cell survival measured after 96 hrs by MTT assay Antiproliferative activity against human PC-3 cells expressing ALDH1A1 and ALDH3A1 assessed as reduction in cell survival measured after 96 hrs by MTT assay	[PMID: 35212533]

In Vitro

4-Diethylaminobenzaldehyde (DEAB) is reversible competitive inhibitor of ALDH1 (competitive with the aldehyde substrate) with a K_i value of 4 nM, and irreversibly inactivates ALDH7A1 via formation of a stable, covalent acyl-enzyme species^[1].
? The Temozolomide (TMZ)-resistant cell line LN18 is treated with a combination of TMZ and 4-Diethylaminobenzaldehyde for 10 days. As indicated by colony formation assay, the clonogenic capacity of LN18 cells exposed to TMZ in combination with 4-Diethylaminobenzaldehyde (DEAB) is significantly attenuated^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

177.24

Formula

C₁₁H₁₅NO

CAS No.

120-21-8

Appearance

Solid

Color

Yellow to brown

SMILES

O=CC1=CC=C(N(CC)CC)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (564.21 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6421 mL	28.2103 mL	56.4207 mL
5 mM	1.1284 mL	5.6421 mL	11.2841 mL
10 mM	0.5642 mL	2.8210 mL	5.6421 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.25 mg/mL (18.34 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) HNMR (118 KB) GC (206 KB)

Handling Instructions (2659 KB)

References

[1]. Luo M, et al. Diethylaminobenzaldehyde is a covalent, irreversible inactivator of ALDH7A1. ACS Chem Biol. 2015 Mar 20;10(3):693-7. [Content Brief]

[2]. Araki N, et al. Screening for androgen receptor activities in 253 industrial chemicals by in vitro reporter gene assays using AR-EcoScreen cells. Toxicol In Vitro. 2005 Sep;19(6):831-42. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6421 mL	28.2103 mL	56.4207 mL	141.0517 mL
	5 mM	1.1284 mL	5.6421 mL	11.2841 mL	28.2103 mL
	10 mM	0.5642 mL	2.8210 mL	5.6421 mL	14.1052 mL
	15 mM	0.3761 mL	1.8807 mL	3.7614 mL	9.4034 mL
	20 mM	0.2821 mL	1.4105 mL	2.8210 mL	7.0526 mL
	25 mM	0.2257 mL	1.1284 mL	2.2568 mL	5.6421 mL
	30 mM	0.1881 mL	0.9403 mL	1.8807 mL	4.7017 mL
	40 mM	0.1411 mL	0.7053 mL	1.4105 mL	3.5263 mL
	50 mM	0.1128 mL	0.5642 mL	1.1284 mL	2.8210 mL
	60 mM	0.0940 mL	0.4702 mL	0.9403 mL	2.3509 mL
	80 mM	0.0705 mL	0.3526 mL	0.7053 mL	1.7631 mL
	100 mM	0.0564 mL	0.2821 mL	0.5642 mL	1.4105 mL