5-O-Methylembelin

Cat. No.: HY-W510159 Purity: 98.79%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5-O-Methylembelin is a natural isocoumarin that inhibits PCSK9, inducible degrader of the low-density lipoprotein receptor (IDLR), and sterol regulatory element binding protein 2 (SREBP2) mRNA expression.

For research use only. We do not sell to patients.

5-O-Methylembelin Chemical Structure

CAS No. : 56005-10-8

Size	Price	Stock	Quantity
5 mg	USD 256	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 410	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 820	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1310	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	9.1 μM Compound: 21b	Inhibition of mPGES1 in IL-1beta stimulated human A549 cell microsomal membranes assessed as reduction in PGE2 synthase activity after 15 mins using PGH2 substrate by RP-HPLC method Inhibition of mPGES1 in IL-1beta stimulated human A549 cell microsomal membranes assessed as reduction in PGE2 synthase activity after 15 mins using PGH2 substrate by RP-HPLC method	[PMID: 25765759]
Bel-7402	IC₅₀	3.6 μg/mL Compound: 8	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	[PMID: 15165134]
HeLa	IC₅₀	9 μg/mL Compound: 8	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 15165134]
HL-60	IC₅₀	3 μg/mL Compound: 8	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 15165134]
U-937	IC₅₀	1.5 μg/mL Compound: 8	Cytotoxicity against human U937 cells after 48 hrs by MTT assay Cytotoxicity against human U937 cells after 48 hrs by MTT assay	[PMID: 15165134]

In Vitro

5-O-Methylembelin (compound 3; 20 μM; 24 h) downregulates PCSK9 (IC₅₀ value of 4.9 μM), IDLR, and SREBP2 mRNA expression significantly^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR_[1]

Cell Line:	HepG2 cells
Concentration:	20 μM
Incubation Time:	24 h
Result:	Inhibited PCSK9, IDOL, and SREBP2 mRNA expression.

Molecular Weight

308.41

Formula

C₁₈H₂₈O₄

CAS No.

56005-10-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

CCCCCCCCCCCC1=C(C(C=C(C1=O)OC)=O)O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (324.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2424 mL	16.2122 mL	32.4244 mL
5 mM	0.6485 mL	3.2424 mL	6.4849 mL
10 mM	0.3242 mL	1.6212 mL	3.2424 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.11 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.11 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (8.11 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.84%

Select Batch:

Data Sheet (268 KB) SDS (393 KB)

COA (241 KB) HNMR (139 KB) RP-HPLC (169 KB) LCMS (362 KB)

Handling Instructions (2659 KB)

References

[1]. Pisey Pel, et al. Isocoumarins and Benzoquinones with Their Proprotein Convertase Subtilisin/Kexin Type 9 Expression Inhibitory Activities from Dried Roots of Lysimachia vulgaris. ACS Omega. 2022 Dec 8;7(50):47296-47305. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2424 mL	16.2122 mL	32.4244 mL	81.0609 mL
	5 mM	0.6485 mL	3.2424 mL	6.4849 mL	16.2122 mL
	10 mM	0.3242 mL	1.6212 mL	3.2424 mL	8.1061 mL
	15 mM	0.2162 mL	1.0808 mL	2.1616 mL	5.4041 mL
	20 mM	0.1621 mL	0.8106 mL	1.6212 mL	4.0530 mL
	25 mM	0.1297 mL	0.6485 mL	1.2970 mL	3.2424 mL
	30 mM	0.1081 mL	0.5404 mL	1.0808 mL	2.7020 mL
	40 mM	0.0811 mL	0.4053 mL	0.8106 mL	2.0265 mL
	50 mM	0.0648 mL	0.3242 mL	0.6485 mL	1.6212 mL
	60 mM	0.0540 mL	0.2702 mL	0.5404 mL	1.3510 mL
	80 mM	0.0405 mL	0.2027 mL	0.4053 mL	1.0133 mL
	100 mM	0.0324 mL	0.1621 mL	0.3242 mL	0.8106 mL