2. Membrane Transporter/Ion Channel
  3. Monocarboxylate Transporter
  4. 7ACC1

7ACC1  (Synonyms: DEAC; Coumarin D 1421; D 1421)

Cat. No.: HY-D0067 Purity: 99.96%
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7ACC1 (DEAC; Coumarin D 1421; D 1421) is a monocarboxylate transporter 1 (MCT-1)/MCT-4 specifc blocker. 7ACC1 attenuates renal cancer cell proliferation, migration, invasion and down-regulates the levels of MCT1/MCT4 expression and extracellular lactate. 7ACC1 is promising for research of cancers.

For research use only. We do not sell to patients.

7ACC1 Chemical Structure

7ACC1 Chemical Structure

CAS No. : 50995-74-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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250 mg USD 32 In-stock
1 g USD 59 In-stock
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Based on 10 publication(s) in Google Scholar

7ACC1 Related Antibodies

Top Publications Citing Use of Products

    7ACC1 purchased from MedChemExpress. Usage Cited in: Cancer Med. 2018 Sep;7(9):4690-4700.  [Abstract]

    The protein expression of MCT4 in the murine breast cancer cell line 4T1 treated with 7ACC1 (0.1 mM) or transfected with different ShMCT4 vectors.

    View All Monocarboxylate Transporter Isoform Specific Products:

    View All Isoforms
    MCT1 MCT2 MCT4

    • Biological Activity

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    Description

    7ACC1 (DEAC; Coumarin D 1421; D 1421) is a monocarboxylate transporter 1 (MCT-1)/MCT-4 specifc blocker. 7ACC1 attenuates renal cancer cell proliferation, migration, invasion and down-regulates the levels of MCT1/MCT4 expression and extracellular lactate. 7ACC1 is promising for research of cancers[1][2][3].

    IC50 & Target

    MCT1

     

    In Vitro

    7ACC1 (10 μM, 0-96 h) attenuates the viability, decreases cell migration and invasion in co-culturing conditions of 786-O cells and HUVECs[1].
    7ACC1 (10 μM, 24 h) inhibits the protein expressions of MCT1 and MCT4, down-regulates the extracellular lactate levels in co-culturing conditions of 786-O cells and HUVECs[1].
    7ACC1 (0-1 μM, 24 h) inhibit the influx but not the efflux of lactate in cancer cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    7ACC1 Related Antibodies

    Western Blot Analysis[1]

    Cell Line: 786-O cells and HUVECs
    Concentration: 10 µM
    Incubation Time: 24 h
    Result: Attenuated the protein expressions of MCT1 and MCT4 in co-culturing of 786-O cells and HUVECs.
    In Vivo

    7ACC1 (3 mg/kg, i.p., daily for 12 days) inhibits the growth of MCT-expressing cervix SiHa, colorectal HCT116 and breast MCF-7 tumors in mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: mice bearing tumors, including cervix cancer SiHa xenografts but also tumors derived from human colon cancer cells HCT116 and bladder cancer cells UM-UC3[2]
    Dosage: 3 mg/kg
    Administration: i.p., daily for 12 days
    Result: Led to significant SiHa and HCT116 tumor growth delays in mice bearing tumors.
    Molecular Weight

    261.27

    Formula

    C14H15NO4
    CAS No.
    Appearance

    Solid

    Color

    Orange to red

    Emission (Em)

    445
    Excitation (Ex)

    345
    SMILES

    O=C(C1=CC2=CC=C(N(CC)CC)C=C2OC1=O)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (127.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.8275 mL 19.1373 mL 38.2746 mL
    5 mM 0.7655 mL 3.8275 mL 7.6549 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (9.57 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.96%

    COA (248 KB) LCMS (266 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.8275 mL 19.1373 mL 38.2746 mL 95.6865 mL
    5 mM 0.7655 mL 3.8275 mL 7.6549 mL 19.1373 mL
    10 mM 0.3827 mL 1.9137 mL 3.8275 mL 9.5686 mL
    15 mM 0.2552 mL 1.2758 mL 2.5516 mL 6.3791 mL
    20 mM 0.1914 mL 0.9569 mL 1.9137 mL 4.7843 mL
    25 mM 0.1531 mL 0.7655 mL 1.5310 mL 3.8275 mL
    30 mM 0.1276 mL 0.6379 mL 1.2758 mL 3.1895 mL
    40 mM 0.0957 mL 0.4784 mL 0.9569 mL 2.3922 mL
    50 mM 0.0765 mL 0.3827 mL 0.7655 mL 1.9137 mL
    60 mM 0.0638 mL 0.3190 mL 0.6379 mL 1.5948 mL
    80 mM 0.0478 mL 0.2392 mL 0.4784 mL 1.1961 mL
    100 mM 0.0383 mL 0.1914 mL 0.3827 mL 0.9569 mL
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    7ACC1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    7ACC150995-74-9DEAC Coumarin D 1421 D 1421Coumarin D1421Coumarin D 1421Coumarin D-1421D1421D 1421D-1421Monocarboxylate TransporterMCT-1MCT-4renal cancer cellextracellular lactatecancersInhibitorinhibitorinhibit

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