2. Cell Cycle/DNA Damage Epigenetics
  3. HDAC
  4. 9-Hydroxyoctadecanoic acid

9-Hydroxyoctadecanoic acid  (Synonyms: 9-Hydroxystearic acid; 9-HSA)

Cat. No.: HY-N11692 Purity: ≥98.0%
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9-Hydroxyoctadecanoic acid (9-HSA) is an HDAC1 inhibitor that inhibits ∼66.4% HDAC1 enzymatic activity at 5 μM. 9-Hydroxyoctadecanoic acid shows anticancer activity.

For research use only. We do not sell to patients.

9-Hydroxyoctadecanoic acid Chemical Structure

9-Hydroxyoctadecanoic acid Chemical Structure

CAS No. : 3384-24-5

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5 mg USD 210 In-stock
10 mg USD 360 In-stock
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Based on 1 publication(s) in Google Scholar

9-Hydroxyoctadecanoic acid Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 9-Hydroxyoctadecanoic acid

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

9-Hydroxyoctadecanoic acid (9-HSA) is an HDAC1 inhibitor that inhibits ∼66.4% HDAC1 enzymatic activity at 5 μM. 9-Hydroxyoctadecanoic acid shows anticancer activity[1].

IC50 & Target[1]

HDAC1

 

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
> 30 μM
Compound: 7
Inhibition of CRM1 interaction with HIV1 HA-tagged Rev in HeLa cells assessed as inhibition of Rev nuclear export by indirect fluorescent antibody technique
Inhibition of CRM1 interaction with HIV1 HA-tagged Rev in HeLa cells assessed as inhibition of Rev nuclear export by indirect fluorescent antibody technique
[PMID: 20176483]
In Vitro

9-Hydroxyoctadecanoic acid (9-HSA) can bind to the active site of the three-dimensional model of the human HDAC1 protein[1].
9-Hydroxyoctadecanoic acid (9-HSA) (100 μM; 24 h) inhibits HT29 cell proliferation, induces arrest in G0/G1, and increases p21WAF1 expression both at the transcriptional and the translational levels[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9-Hydroxyoctadecanoic acid Related Antibodies

Cell Proliferation Assay9-Hydroxyoctadecanoic acid 可以结合到人 HDAC1 蛋白三维模型的活性位点[1]。

Cell Line: HT29 cell
Concentration: 100 μM
Incubation Time: 24 h
Result: Resulted in a significant inhibition of cell proliferation.

Cell Cycle Analysis9-Hydroxyoctadecanoic acid 可以结合到人 HDAC1 蛋白三维模型的活性位点[1]。

Cell Line: HT29 cell
Concentration: 100 μM
Incubation Time: 24 h
Result: Decreased S-phase activity by 50.2% compared with untreated controls, and the growth inhibition was associated with a strong arrest in G0/G1.

Western Blot Analysis9-Hydroxyoctadecanoic acid 可以结合到人 HDAC1 蛋白三维模型的活性位点[1]。

Cell Line: HT29 cell
Concentration: 100 μM
Incubation Time: 24 h
Result: Increased the expression of p21WAF1.

RT-PCR9-Hydroxyoctadecanoic acid 可以结合到人 HDAC1 蛋白三维模型的活性位点[1]。

Cell Line: HT29 cell
Concentration: 100 μM
Incubation Time: 24 h
Result: Induced p21WAF1 tanscript.
Molecular Weight

300.48

Formula

C18H36O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(CCCCCCCC(CCCCCCCCC)O)O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (332.80 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3280 mL 16.6400 mL 33.2801 mL
5 mM 0.6656 mL 3.3280 mL 6.6560 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3280 mL 16.6400 mL 33.2801 mL 83.2002 mL
5 mM 0.6656 mL 3.3280 mL 6.6560 mL 16.6400 mL
10 mM 0.3328 mL 1.6640 mL 3.3280 mL 8.3200 mL
15 mM 0.2219 mL 1.1093 mL 2.2187 mL 5.5467 mL
20 mM 0.1664 mL 0.8320 mL 1.6640 mL 4.1600 mL
25 mM 0.1331 mL 0.6656 mL 1.3312 mL 3.3280 mL
30 mM 0.1109 mL 0.5547 mL 1.1093 mL 2.7733 mL
40 mM 0.0832 mL 0.4160 mL 0.8320 mL 2.0800 mL
50 mM 0.0666 mL 0.3328 mL 0.6656 mL 1.6640 mL
60 mM 0.0555 mL 0.2773 mL 0.5547 mL 1.3867 mL
80 mM 0.0416 mL 0.2080 mL 0.4160 mL 1.0400 mL
100 mM 0.0333 mL 0.1664 mL 0.3328 mL 0.8320 mL
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9-Hydroxyoctadecanoic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

9-Hydroxyoctadecanoic acid3384-24-59-Hydroxystearic acid 9-HSAHDACHistone deacetylasesHT29p21 WAF1anticancerInhibitorinhibitorinhibit

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