2. Protein Tyrosine Kinase/RTK
  3. Insulin Receptor
  4. AGL-2263

AGL-2263 is an insulin receptor and insulin-like growth factor (IGF) receptor inhibitor.

For research use only. We do not sell to patients.

AGL-2263 Chemical Structure

AGL-2263 Chemical Structure

CAS No. : 638213-98-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 106 In-stock
Solution
10 mM * 1 mL in DMSO USD 106 In-stock
Solid
5 mg USD 150 In-stock
10 mg USD 240 In-stock
25 mg USD 490 In-stock
50 mg USD 850 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

AGL-2263 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE AGL-2263

WB

    AGL-2263 purchased from MedChemExpress. Usage Cited in: Reprod Biol. 2019 Sep;19(3):293-298.  [Abstract]

    Granulosa cells pretreated with AG1024 (10 or 5 μM, inhibitor of IGF-1R autophosphorylation) for 1 h before insulin stimulation for 24 h. Levels of P-AKT, P-ERK 1/2, and β-actin detected in whole-cell lysates.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    AGL-2263 is an insulin receptor and insulin-like growth factor (IGF) receptor inhibitor[1][2].

    IC50 & Target

    Insulin receptor[1].
    In Vitro

    AGL-2263 (5 μM, for 1 h followed by insulin treatment for 24 h) inhibits IR and insulin could still induce phosphorylation of AKT and ERK1/2 after the IR inhibition[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    AGL-2263 Related Antibodies

    Western Blot Analysis[3]

    Cell Line: Granulosa cells.
    Concentration: 5 μM.
    Incubation Time: For 1 h followed by insulin treatment for 24 h.
    Result: Insulin still induced phosphorylation of AKT and ERK1/2 after IR was inhibited by AGL2263.
    Molecular Weight

    322.27

    Formula

    C17H10N2O5
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=CC(O)=C(O)C=C1)/C(C#N)=C/C2=CC=C(OC3=O)C(N3)=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (310.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1030 mL 15.5149 mL 31.0299 mL
    5 mM 0.6206 mL 3.1030 mL 6.2060 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.45 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.45 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.26%

    SDS (251 KB)

    COA (246 KB) HNMR (276 KB) LCMS (96 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1030 mL 15.5149 mL 31.0299 mL 77.5747 mL
    5 mM 0.6206 mL 3.1030 mL 6.2060 mL 15.5149 mL
    10 mM 0.3103 mL 1.5515 mL 3.1030 mL 7.7575 mL
    15 mM 0.2069 mL 1.0343 mL 2.0687 mL 5.1716 mL
    20 mM 0.1551 mL 0.7757 mL 1.5515 mL 3.8787 mL
    25 mM 0.1241 mL 0.6206 mL 1.2412 mL 3.1030 mL
    30 mM 0.1034 mL 0.5172 mL 1.0343 mL 2.5858 mL
    40 mM 0.0776 mL 0.3879 mL 0.7757 mL 1.9394 mL
    50 mM 0.0621 mL 0.3103 mL 0.6206 mL 1.5515 mL
    60 mM 0.0517 mL 0.2586 mL 0.5172 mL 1.2929 mL
    80 mM 0.0388 mL 0.1939 mL 0.3879 mL 0.9697 mL
    100 mM 0.0310 mL 0.1551 mL 0.3103 mL 0.7757 mL
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    AGL-2263 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    AGL-2263638213-98-6AGL2263AGL 2263Insulin ReceptorInhibitorinhibitorinhibit

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