2. Anti-infection Metabolic Enzyme/Protease
  3. Antibiotic Endogenous Metabolite
  4. Azelaic acid

Azelaic acid  (Synonyms: Nonanedioic acid)

Cat. No.: HY-B0704 Purity: ≥98.0%
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Azelaic acid is a nine-carbon dicarboxylic acid. Azelaic acid has antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis through inhibition of microbial cellular prorein synthesis. Azelaic acid has hypopigmentation action resulting from its ability to scavenge free radicals.

For research use only. We do not sell to patients.

Azelaic acid Chemical Structure

Azelaic acid Chemical Structure

CAS No. : 123-99-9

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Based on 1 publication(s) in Google Scholar

Other Forms of Azelaic acid:

Azelaic acid Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Azelaic acid

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  • Purity & Documentation

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Description

Azelaic acid is a nine-carbon dicarboxylic acid. Azelaic acid has antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis through inhibition of microbial cellular prorein synthesis. Azelaic acid has hypopigmentation action resulting from its ability to scavenge free radicals[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Azelaic acid (0.5 M; 48 h-7 D) has antimicrobial effect[3].
Azelaic acid (5 M; 24 h) decreases intracellular Reactive Oxygen Species (ROS) levels and increases antioxidant capacity[5].
Azelaic acid (1-100 nM, 24 h) suppresses B16, HMB2, and SK23 cell survival ability in a dose-dependent manner[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Azelaic acid Related Antibodies

Cell Viability Assay[3]

Cell Line: Propionibacterium acnes and Staphylococcus epidermidis
Concentration: 0.5 M
Incubation Time: Propionibacterium acnes for 48 h, Staphylococcus epidermidis for 7 D
Result: Exhibited large reduction in viability (at least 40-fold) over 24 h.

Immunofluorescence[5]

Cell Line: HL60, U937, THP-1, and AML-PC cells
Concentration: 5 M
Incubation Time: 24 h
Result: Exhibited markedly decreasing the intracellular ROS levels.

Cell Viability Assay[6]

Cell Line: B16, HMB2, and SK23, CHO
Concentration: 10 nM, 20 nM, 30 nM, 40 nM, 50 nM, 60 nM, 70 nM, 80 nM, 90 nM, 100 nM
Incubation Time: 24 h
Result: Showed significantly reduce in the number of B16, HMB2, and SK23 comparing to CHO.
In Vivo

Azelaic acid (15% gel; Smear; twice daily) in 15% gel is efficacious in people with moderate papulo-pustular[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Human with rosacea for 12 weeks[4]
Dosage: 15% gel
Administration: Smear
Result: Showed excellent improvent in 78% of the patients with Azelaic acid.
Clinical Trial
Molecular Weight

188.22

Formula

C9H16O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC(CCCCCCCC(O)=O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (531.29 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 2 mg/mL (10.63 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.3129 mL 26.5647 mL 53.1293 mL
5 mM 1.0626 mL 5.3129 mL 10.6259 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (13.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (13.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (240 KB) HNMR (114 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 5.3129 mL 26.5647 mL 53.1293 mL 132.8233 mL
5 mM 1.0626 mL 5.3129 mL 10.6259 mL 26.5647 mL
10 mM 0.5313 mL 2.6565 mL 5.3129 mL 13.2823 mL
DMSO 15 mM 0.3542 mL 1.7710 mL 3.5420 mL 8.8549 mL
20 mM 0.2656 mL 1.3282 mL 2.6565 mL 6.6412 mL
25 mM 0.2125 mL 1.0626 mL 2.1252 mL 5.3129 mL
30 mM 0.1771 mL 0.8855 mL 1.7710 mL 4.4274 mL
40 mM 0.1328 mL 0.6641 mL 1.3282 mL 3.3206 mL
50 mM 0.1063 mL 0.5313 mL 1.0626 mL 2.6565 mL
60 mM 0.0885 mL 0.4427 mL 0.8855 mL 2.2137 mL
80 mM 0.0664 mL 0.3321 mL 0.6641 mL 1.6603 mL
100 mM 0.0531 mL 0.2656 mL 0.5313 mL 1.3282 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Azelaic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Azelaic acid123-99-9Nonanedioic acidAntibioticEndogenous MetaboliteInhibitorinhibitorinhibit

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