2. Anti-infection
  3. Bacterial Antibiotic
  4. Cefazolin sodium

Cefazolin sodium  (Synonyms: Cephazolin sodium)

Cat. No.: HY-B1078 Purity: 99.08%
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Cefazolin sodium is a first-generation cephalosporin antibiotic and can be used in varieties of bacterial infections research. Cefazolin sodium has anti-inflammatory effect and can attenuate post-operative cognitive dysfunction (POCD).

For research use only. We do not sell to patients.

Cefazolin sodium Chemical Structure

Cefazolin sodium Chemical Structure

CAS No. : 27164-46-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 7 publication(s) in Google Scholar

Other Forms of Cefazolin sodium:

Cefazolin sodium Related Antibodies

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  • Purity & Documentation

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Description

Cefazolin sodium is a first-generation cephalosporin antibiotic and can be used in varieties of bacterial infections research[1]. Cefazolin sodium has anti-inflammatory effect and can attenuate post-operative cognitive dysfunction (POCD)[2].

IC50 & Target

β-lactam

 

In Vitro

Cefazolin sodium (0-300 μg/ml; 6 or 24 h) has a direct anti-inflammatory effect on C8-B4 cells stimulated by lipopolysaccharide[2].
Cefazolin sodium (0-400 μM; 72 h) treatment inhibits IL-2, IL-4 and IL-15-induced cell proliferation[3].
Cefazolin sodium (0-400 μM; 30 min) treatment inhibits IL-2, IL-4, IL-15 and IL-21-stimulated JAK3 phosphorylation[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cefazolin sodium Related Antibodies

Cell Viability Assay[2]

Cell Line: C8-B4 cells
Concentration: 0, 50, 100, 150, 200, 250, or 300 μg/ml
Incubation Time: 6 or 24 hours
Result: Inhibited the increase of IL-1β at all doses, but inhibited the increase of IL-6 only at 200 μg/ml.

Cell Proliferation Assay[3]

Cell Line: PBMC, and TF-1 cells
Concentration: 0, 100, 200, and 400 μM
Incubation Time: 72 hours
Result: Reduced IL-2, IL-4 and IL-15-induced cell proliferation, suggested that Cefazolin interferes not only with IL-15Rα, but also with IL-2/IL-15Rβ and/or γc.

Cell Viability Assay[3]

Cell Line: PBMC, NK-92, and TF-1 cells
Concentration: 0, 100, 200, and 400 μM
Incubation Time: 30 min
Result: Diminished the phosphorylation of JAK3 in response to the cytokine treatment, concluded suppressing signal transduction by γc receptors.
In Vivo

Cefazolin sodium (Subcutaneous injection; 300-500 mg/kg; once daily; 5 d) treatment improves learning and memory in mice after surgery[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 6- to 8-week-old male CD-1 mice underwent clinical exploratory laparotomy[2]
Dosage: 300-500 mg/kg
Administration: Subcutaneous injection; 300-500 mg/kg; once daily; 5 days
Result: Attenuated learning and memory dysfunction induced by the surgery.
Clinical Trial
Molecular Weight

476.49

Formula

C14H13N8NaO4S3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C(N12)=C(CSC3=NN=C(C)S3)CS[C@]2([H])[C@H](NC(CN4N=NN=C4)=O)C1=O)O[Na]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (209.87 mM)

DMSO : 100 mg/mL (209.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0987 mL 10.4934 mL 20.9868 mL
5 mM 0.4197 mL 2.0987 mL 4.1974 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (209.87 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.0987 mL 10.4934 mL 20.9868 mL 52.4670 mL
5 mM 0.4197 mL 2.0987 mL 4.1974 mL 10.4934 mL
10 mM 0.2099 mL 1.0493 mL 2.0987 mL 5.2467 mL
15 mM 0.1399 mL 0.6996 mL 1.3991 mL 3.4978 mL
20 mM 0.1049 mL 0.5247 mL 1.0493 mL 2.6233 mL
25 mM 0.0839 mL 0.4197 mL 0.8395 mL 2.0987 mL
30 mM 0.0700 mL 0.3498 mL 0.6996 mL 1.7489 mL
40 mM 0.0525 mL 0.2623 mL 0.5247 mL 1.3117 mL
50 mM 0.0420 mL 0.2099 mL 0.4197 mL 1.0493 mL
60 mM 0.0350 mL 0.1749 mL 0.3498 mL 0.8744 mL
80 mM 0.0262 mL 0.1312 mL 0.2623 mL 0.6558 mL
100 mM 0.0210 mL 0.1049 mL 0.2099 mL 0.5247 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Cefazolin sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cefazolin27164-46-1CephazolinBacterialAntibioticanti-inflammatory activitypost-operative cognitive dysfunctionPOCDC8-B4PBMCNK-92TF-1Inhibitorinhibitorinhibit

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