2. Metabolic Enzyme/Protease
  3. Carbonic Anhydrase
  4. Dichlorphenamide

Dichlorphenamide  (Synonyms: Diclofenamide)

Cat. No.: HY-B0397 Purity: 98.90%
COA Handling Instructions

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Dichlorphenamide (Diclofenamide) is an orally active, specific, carbonic anhydrase inhibitor. Dichlorphenamide can reduce intraocular pressure by inhibiting the secretion of water from the eye. Dichlorphenamide can be used for glaucoma research.

For research use only. We do not sell to patients.

Dichlorphenamide Chemical Structure

Dichlorphenamide Chemical Structure

CAS No. : 120-97-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 101 In-stock
Solution
10 mM * 1 mL in DMSO USD 101 In-stock
Solid
5 mg USD 59 In-stock
10 mg USD 92 In-stock
50 mg USD 120 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Dichlorphenamide:

Dichlorphenamide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Dichlorphenamide

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CA CA I CA Ⅱ CA Ⅸ CA XII

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Dichlorphenamide (Diclofenamide) is an orally active, specific, carbonic anhydrase inhibitor. Dichlorphenamide can reduce intraocular pressure by inhibiting the secretion of water from the eye. Dichlorphenamide can be used for glaucoma research[1].

In Vivo

Dichlorphenamide can reduce IOP locally in male albino rabbits[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male albino rabbits (approximately 2.5 kg)
Dosage: 50 μL 10% aqueous solutions of dichlorphenamide sodium or 2 mg/kg, 6 mg/kg
Administration: Eye drop of 50 μL or Oral gavage of 2 mg/kg or 6 mg/kg, 5 hours
Result: Showed significant decrease in IOP at 30 min by instilling into eyes and decrease in IOP only at 1 h by orally after drug administration, and demonstrated that drug levels were significantly higher in the iris ciliary body and lower in the serum by instilling compared to oral administration.
Clinical Trial
Molecular Weight

305.16

Formula

C6H6Cl2N2O4S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC(Cl)=C(Cl)C(S(=O)(N)=O)=C1)(N)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (327.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2770 mL 16.3848 mL 32.7697 mL
5 mM 0.6554 mL 3.2770 mL 6.5539 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.90%

COA (252 KB) RP-HPLC (260 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2770 mL 16.3848 mL 32.7697 mL 81.9242 mL
5 mM 0.6554 mL 3.2770 mL 6.5539 mL 16.3848 mL
10 mM 0.3277 mL 1.6385 mL 3.2770 mL 8.1924 mL
15 mM 0.2185 mL 1.0923 mL 2.1846 mL 5.4616 mL
20 mM 0.1638 mL 0.8192 mL 1.6385 mL 4.0962 mL
25 mM 0.1311 mL 0.6554 mL 1.3108 mL 3.2770 mL
30 mM 0.1092 mL 0.5462 mL 1.0923 mL 2.7308 mL
40 mM 0.0819 mL 0.4096 mL 0.8192 mL 2.0481 mL
50 mM 0.0655 mL 0.3277 mL 0.6554 mL 1.6385 mL
60 mM 0.0546 mL 0.2731 mL 0.5462 mL 1.3654 mL
80 mM 0.0410 mL 0.2048 mL 0.4096 mL 1.0241 mL
100 mM 0.0328 mL 0.1638 mL 0.3277 mL 0.8192 mL
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Dichlorphenamide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dichlorphenamide120-97-8DiclofenamideCarbonic AnhydraseCarbonate dehydrataseInhibitorGlaucomaEyesinhibitorinhibit

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