2. Metabolic Enzyme/Protease
  3. Carbonic Anhydrase
  4. Dimethylfraxetin

Dimethylfraxetin  (Synonyms: 6,7,8-Trimethoxycoumarin; Fraxetin dimethyl ether)

Cat. No.: HY-N0085 Purity: 99.81%
COA Handling Instructions

Molarity Calculator

Dimethylfraxetin is a Carbonic anhydrase inhibitor, with a Ki value of 0.0097 μM.

For research use only. We do not sell to patients.

Dimethylfraxetin Chemical Structure

Dimethylfraxetin Chemical Structure

CAS No. : 6035-49-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 132 In-stock
Solution
10 mM * 1 mL in DMSO USD 132 In-stock
Solid
1 mg USD 54 In-stock
5 mg USD 120 In-stock
10 mg USD 197 In-stock
25 mg USD 395 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

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Customer Review

Based on 1 publication(s) in Google Scholar

Dimethylfraxetin Related Antibodies

Top Publications Citing Use of Products

View All Carbonic Anhydrase Isoform Specific Products:

View All Isoforms
CA CA I CA Ⅱ CA Ⅸ CA XII

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Dimethylfraxetin is a Carbonic anhydrase inhibitor, with a Ki value of 0.0097 μM.

IC50 & Target

Ki: 0.0097 μM (Carbonic anhydrase)[1]
In Vitro

At CA I there is one stand out compound being Dimethylfraxetin (compound 17), a nanomolar CA I inhibitor. This trimethoxy coumarin is the most potent of any of the NP coumarins across the six CA isozymes of the present study[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dimethylfraxetin Related Antibodies
Molecular Weight

236.22

Formula

C12H12O5
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C1C=CC2=CC(OC)=C(OC)C(OC)=C2O1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (529.17 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.2333 mL 21.1667 mL 42.3334 mL
5 mM 0.8467 mL 4.2333 mL 8.4667 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.97%

COA (251 KB) HNMR (170 KB) LCMS (406 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[1]

Inhibitor (including Dimethylfraxetin) and enzyme solutions are preincubated together for 6 h at room temperature prior to assay, in order to allow for the formation of the enzyme-inhibitor complex[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.2333 mL 21.1667 mL 42.3334 mL 105.8335 mL
5 mM 0.8467 mL 4.2333 mL 8.4667 mL 21.1667 mL
10 mM 0.4233 mL 2.1167 mL 4.2333 mL 10.5834 mL
15 mM 0.2822 mL 1.4111 mL 2.8222 mL 7.0556 mL
20 mM 0.2117 mL 1.0583 mL 2.1167 mL 5.2917 mL
25 mM 0.1693 mL 0.8467 mL 1.6933 mL 4.2333 mL
30 mM 0.1411 mL 0.7056 mL 1.4111 mL 3.5278 mL
40 mM 0.1058 mL 0.5292 mL 1.0583 mL 2.6458 mL
50 mM 0.0847 mL 0.4233 mL 0.8467 mL 2.1167 mL
60 mM 0.0706 mL 0.3528 mL 0.7056 mL 1.7639 mL
80 mM 0.0529 mL 0.2646 mL 0.5292 mL 1.3229 mL
100 mM 0.0423 mL 0.2117 mL 0.4233 mL 1.0583 mL
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Dimethylfraxetin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dimethylfraxetin6035-49-06,7,8-Trimethoxycoumarin Fraxetin dimethyl etherCarbonic AnhydraseCarbonate dehydrataseInhibitorinhibitorinhibit

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