Dimethylfraxetin (Synonyms: 6,7,8-Trimethoxycoumarin; Fraxetin dimethyl ether)

Name: Dimethylfraxetin
Brand: MedChemExpress
SKU: HY-N0085
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0085 Purity: 99.81%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Dimethylfraxetin is a Carbonic anhydrase inhibitor, with a K_i value of 0.0097 μM.

For research use only. We do not sell to patients.

Dimethylfraxetin Chemical Structure

CAS No. : 6035-49-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 132	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 132	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 54	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 197	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 395	In-stock	Estimated Time of Arrival: December 31
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100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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CA CA I CA Ⅱ CA Ⅸ CA XII

Biological Activity
Protocol
Purity & Documentation
References
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Description

Dimethylfraxetin is a Carbonic anhydrase inhibitor, with a K_i value of 0.0097 μM.

IC₅₀ & Target

Ki: 0.0097 μM (Carbonic anhydrase)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Neutrophil	IC₅₀	> 30 μM Compound: 7	Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced superoxide anion generation treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge by spectrophotometry Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced superoxide anion generation treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge by spectrophotometry	[PMID: 19203247]
Neutrophil	IC₅₀	47.1 μM Compound: 15	Antiinflammatory activity in human neutrophils assessed as fMLP-induced superoxide release after 5 mins by spectrometry Antiinflammatory activity in human neutrophils assessed as fMLP-induced superoxide release after 5 mins by spectrometry	[PMID: 17822293]

In Vitro

At CA I there is one stand out compound being Dimethylfraxetin (compound 17), a nanomolar CA I inhibitor. This trimethoxy coumarin is the most potent of any of the NP coumarins across the six CA isozymes of the present study^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

236.22

Formula

C₁₂H₁₂O₅

CAS No.

6035-49-0

Appearance

Solid

Color

White to yellow

SMILES

O=C1C=CC2=CC(OC)=C(OC)C(OC)=C2O1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (529.17 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2333 mL	21.1667 mL	42.3334 mL
5 mM	0.8467 mL	4.2333 mL	8.4667 mL
10 mM	0.4233 mL	2.1167 mL	4.2333 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (275 KB) SDS (392 KB)

COA (251 KB) HNMR (170 KB) LCMS (406 KB)

Handling Instructions (2659 KB)

References

[1]. Davis RA, et al. Natural product coumarins that inhibit human carbonic anhydrases. Bioorg Med Chem. 2013 Mar 15;21(6):1539-43. [Content Brief]

Kinase Assay ^[1]	Inhibitor (including Dimethylfraxetin) and enzyme solutions are preincubated together for 6 h at room temperature prior to assay, in order to allow for the formation of the enzyme-inhibitor complex^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Davis RA, et al. Natural product coumarins that inhibit human carbonic anhydrases. Bioorg Med Chem. 2013 Mar 15;21(6):1539-43. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.2333 mL	21.1667 mL	42.3334 mL	105.8335 mL
	5 mM	0.8467 mL	4.2333 mL	8.4667 mL	21.1667 mL
	10 mM	0.4233 mL	2.1167 mL	4.2333 mL	10.5834 mL
	15 mM	0.2822 mL	1.4111 mL	2.8222 mL	7.0556 mL
	20 mM	0.2117 mL	1.0583 mL	2.1167 mL	5.2917 mL
	25 mM	0.1693 mL	0.8467 mL	1.6933 mL	4.2333 mL
	30 mM	0.1411 mL	0.7056 mL	1.4111 mL	3.5278 mL
	40 mM	0.1058 mL	0.5292 mL	1.0583 mL	2.6458 mL
	50 mM	0.0847 mL	0.4233 mL	0.8467 mL	2.1167 mL
	60 mM	0.0706 mL	0.3528 mL	0.7056 mL	1.7639 mL
	80 mM	0.0529 mL	0.2646 mL	0.5292 mL	1.3229 mL
	100 mM	0.0423 mL	0.2117 mL	0.4233 mL	1.0583 mL

Dimethylfraxetin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dimethylfraxetin6035-49-06,7,8-Trimethoxycoumarin Fraxetin dimethyl etherCarbonic AnhydraseCarbonate dehydrataseInhibitorinhibitorinhibit

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Dimethylfraxetin (Synonyms: 6,7,8-Trimethoxycoumarin; Fraxetin dimethyl ether)

Customer Review

Dimethylfraxetin Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Carbonic Anhydrase Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Dimethylfraxetin Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Dimethylfraxetin Related Classifications

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