2. Autophagy Apoptosis
  3. Autophagy Apoptosis
  4. Dioscin

Dioscin  (Synonyms: Collettiside III; CCRIS 4123)

Cat. No.: HY-N0124 Purity: 99.95%
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Dioscin (CCRIS 4123; Collettiside III) is a natural plant-derived steroidal saponin that has good anti-cancer activity against a variety of cancer cells. Dioscin causes DNA damage and induces apoptosis in HeLa and SiHa cells. Dioscin regulates ROS-mediated DNA damage and mitochondrial signaling pathways, exerting anticancer activity.

For research use only. We do not sell to patients.

Dioscin Chemical Structure

Dioscin Chemical Structure

CAS No. : 19057-60-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 84 In-stock
Solution
10 mM * 1 mL in DMSO USD 84 In-stock
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5 mg USD 55 In-stock
10 mg USD 88 In-stock
50 mg USD 319 In-stock
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Based on 12 publication(s) in Google Scholar

Dioscin Related Antibodies

Top Publications Citing Use of Products

    Dioscin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Feb 19;117:109839.  [Abstract]

    Dioscin-Medium group (5 mg/kg; p.o.; every-two days for 12 weeks) profoundly inhibits the phosphorylation of NF-ĸB in mice (Fig H and I). Dioscin-Low (Dio-L, 2.5 mg/kg), Dioscin-Medium (Dio-M, 5 mg/kg) and Dioscin-High (Dio-H, 10 mg/Kg).

    Dioscin purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2017 Oct;16(4):4922-4926.  [Abstract]

    Dioscin concentrations of 0.5, 1, 5, 10 and 25 µg/mL promotes the expression of ALP and inhibits the expression of LC3B in MC3T3-E1 cells, in a concentration-dependent manner.

    • Biological Activity

    • Purity & Documentation

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    • Customer Review

    Description

    Dioscin (CCRIS 4123; Collettiside III) is a natural plant-derived steroidal saponin that has good anti-cancer activity against a variety of cancer cells. Dioscin causes DNA damage and induces apoptosis in HeLa and SiHa cells. Dioscin regulates ROS-mediated DNA damage and mitochondrial signaling pathways, exerting anticancer activity[1][2][3][4][5][6].

    In Vitro

    Dioscin (1.25-5 μg/mL; 6-24 h) increases intracellular calcium levels and induces apoptosis in HeLa and SiHa cells[4].
    Dioscin (1.25-5 μg/mL; 6-24 h) Dioscin up-regulates the protein levels of Bak, Bax, Bid, p53, caspase-3, caspase-9, and down-regulates the proteins of Bcl-2 and Bcl-xl level[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Dioscin Related Antibodies
    In Vivo

    Dioscin (75-300 mg/kg, 10 mL/kg; oral gavage; 90 days) has no subchronic toxicity in female rats and mild subchronic toxicity in male rats. Dioscin caused mild gastrointestinal dilatation in male rats and demonstrated hemolytic anemia on hematological evaluation[5].
    Dioscin can reduce liver ischemia-reperfusion injury in rats by inhibiting oxidative-nitrative stress, inflammation and apoptosis[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    869.04

    Formula

    C45H72O16
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@@H]1[C@@H](O)[C@H](C)O[C@@]([H])(O[C@H]2[C@H](O)[C@@H](O[C@]3([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O)[C@@](O[C@@H]4CC5=CC[C@]([C@]([H])(C[C@@]6([H])[C@]7([H])[C@H](C)[C@]8(OC[C@H](C)CC8)O6)[C@]7(C)CC9)([H])[C@@]9([H])[C@@]5(C)CC4)([H])O[C@@H]2CO)[C@@H]1O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (115.07 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.1507 mL 5.7535 mL 11.5070 mL
    5 mM 0.2301 mL 1.1507 mL 2.3014 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.25 mg/mL (2.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.25 mg/mL (2.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.95%

    COA (256 KB) HNMR (340 KB) RP-HPLC (213 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.1507 mL 5.7535 mL 11.5070 mL 28.7674 mL
    5 mM 0.2301 mL 1.1507 mL 2.3014 mL 5.7535 mL
    10 mM 0.1151 mL 0.5753 mL 1.1507 mL 2.8767 mL
    15 mM 0.0767 mL 0.3836 mL 0.7671 mL 1.9178 mL
    20 mM 0.0575 mL 0.2877 mL 0.5753 mL 1.4384 mL
    25 mM 0.0460 mL 0.2301 mL 0.4603 mL 1.1507 mL
    30 mM 0.0384 mL 0.1918 mL 0.3836 mL 0.9589 mL
    40 mM 0.0288 mL 0.1438 mL 0.2877 mL 0.7192 mL
    50 mM 0.0230 mL 0.1151 mL 0.2301 mL 0.5753 mL
    60 mM 0.0192 mL 0.0959 mL 0.1918 mL 0.4795 mL
    80 mM 0.0144 mL 0.0719 mL 0.1438 mL 0.3596 mL
    100 mM 0.0115 mL 0.0575 mL 0.1151 mL 0.2877 mL
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    Dioscin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Dioscin19057-60-4Collettiside III CCRIS 4123CCRIS4123CCRIS 4123CCRIS-4123AutophagyApoptosisDNA damageROSapoptosiscervical carcinomaNatural productSprague-Dawley rats90-Day subchronic toxicityCardiac diseasesDiosgeninPotential protectiveInhibitorinhibitorinhibit

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