Ecteinascidin 770 (Synonyms: Ecteinascidine 770; Et-770)

Name: Ecteinascidin 770
Brand: MedChemExpress
SKU: HY-101191
Rating: 5.0 (2 reviews)

Cat. No.: HY-101191 Purity: 99.76%: FAQs
Data Sheet SDS COA Handling Instructions

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Ecteinascidin 770 (ET-770) is a 1,2,3,4-tetrahydroisoquinoline alkaloid with potent anti-cancer activities; inhibits U373MG cells with an IC₅₀ of 4.83 nM.

For research use only. We do not sell to patients.

Ecteinascidin 770 Chemical Structure

CAS No. : 114899-80-8

Size	Price	Stock	Quantity
1 mg	USD 260	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 750	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1200	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 2450	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Ecteinascidin 770

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Biological Activity
Protocol
Purity & Documentation
References
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Description

Ecteinascidin 770 (ET-770) is a 1,2,3,4-tetrahydroisoquinoline alkaloid with potent anti-cancer activities; inhibits U373MG cells with an IC₅₀ of 4.83 nM.

IC₅₀ & Target

IC50: 4.83 nM (U373MG cell)^[1]; 0.6 nM (HCT116 cell), 2.4 nM (QG56 cell), 0.81 nM (DU145)^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
DLD-1	IC₅₀	2.4 nM Compound: ecteinascidin 770	Cytotoxicity against human DLD1 cells after 4 days Cytotoxicity against human DLD1 cells after 4 days	[PMID: 14640515]
DU-145	IC₅₀	0.81 nM Compound: 1b	Cytotoxicity against human DU145 cells after 4 days by MTT assay Cytotoxicity against human DU145 cells after 4 days by MTT assay	[PMID: 21752654]
HCT-116	IC₅₀	0.6 nM Compound: 1b	Cytotoxicity against human HCT116 cells after 4 days by MTT assay Cytotoxicity against human HCT116 cells after 4 days by MTT assay	[PMID: 21752654]
HCT-116	IC₅₀	1.2 nM Compound: ecteinascidin 770	Cytotoxicity against human HCT116 cells after 4 days Cytotoxicity against human HCT116 cells after 4 days	[PMID: 14640515]
NCI-H460	IC₅₀	0.64 nM Compound: ecteinascidin 770	Cytotoxicity against human NCI-H460 cells after 4 days Cytotoxicity against human NCI-H460 cells after 4 days	[PMID: 14640515]
QG-56	IC₅₀	2.4 nM Compound: 1b	Cytotoxicity against human QG56 cells after 4 days by MTT assay Cytotoxicity against human QG56 cells after 4 days by MTT assay	[PMID: 21752654]
QG-56	IC₅₀	3.9 nM Compound: ecteinascidin 770	Cytotoxicity against human QG56 cells after 4 days Cytotoxicity against human QG56 cells after 4 days	[PMID: 14640515]

In Vitro

Ecteinascidin 770 induces apoptosis of U373MG cells. The IC₅₀ concentration of ecteinascidin 770 for killing U373MG glioblastoma cells in culture by using the MTT assay is 4.83 nM by a 72 hour-treatment^[1]. The IC₅₀ values against human cell lines HCT116, QG56, and DU145 are 0.6, 2.4, and 0.81 nM, respectively^[2]. ET-770 is shown to enhance anoikis response of human lung cancer H23 cells in a dose-dependent manner. Ecteinascidin 770 sensitizes the cells by activating the p53 protein, which in turn down-regulates anti-apoptotic myeloid cell leukemia sequence-1 (MCL1) and up-regulates BCL2-associated X protein (BAX) proteins. However, B-cell lymphoma-2 (BCL2) proteins are not significantly affected by Ecteinascidin 770. The anoikis sensitization of ET-770 is observed in H460 lung cancer cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

770.85

Formula

C₄₀H₄₂N₄O₁₀S

CAS No.

114899-80-8

Appearance

Solid

Color

White to off-white

SMILES

N#C[C@@H]([C@@]1([H])N(C)[C@]2([H])C3=C(C=C(C)C(OC)=C3O)C1)N4[C@@]2([H])[C@@](SC[C@@]5(NCC6)C7=C6C=C(O)C(OC)=C7)([H])C8=C(C(OCO9)=C9C(C)=C8OC(C)=O)[C@]4([H])COC5=O

Structure Classification

Initial Source

Microorganisms

Ecteinascidia thurstoni

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (64.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2973 mL	6.4863 mL	12.9727 mL
5 mM	0.2595 mL	1.2973 mL	2.5945 mL
10 mM	0.1297 mL	0.6486 mL	1.2973 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.24 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (258 KB) HNMR (503 KB) RP-HPLC (269 KB) LCMS (129 KB)

Handling Instructions (2659 KB)

References

[1]. Tabunoki H, et al. Molecular network profiling of U373MG human glioblastoma cells following induction of apoptosis by novel marine-derived anti-cancer 1,2,3,4-tetrahydroisoquinoline alkaloids. Cancer Cell Int. 2012 Apr 11;12(1):14. [Content Brief]

[2]. Saktrakulkla P, et al. Chemistry of ecteinascidins. Part 3: preparation of 2'-N-acyl derivatives of ecteinascidin 770 and evaluation of cytotoxicity. Bioorg Med Chem. 2011 Aug 1;19(15):4421-36. [Content Brief]

[3]. Powan P, et al. Ecteinascidin 770, a tetrahydroisoquinoline alkaloid, sensitizes human lung cancer cells to anoikis. Anticancer Res. 2013 Feb;33(2):505-12. [Content Brief]

Cell Assay
^[3]

Ecteinascidin 770 is dissolved in DMSO and diluted with appropriate medium. H23 and H460 cells are seeded into 96-well plates at 1×10⁵ cell/mL for 24 h and then treated with different concentrations of ecteinascidin 770 for 24 h. Cells are then incubated with 20 μM of XTT reagent for a further 4 h at 37°C. The intensity of the formazan product is measured at 450 nm using a microplate reader. The cell viability is calculated from the optical density (OD) ratio of treated to non-treated control cells and is presented as a percentage to that of the non-treated controls^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Tabunoki H, et al. Molecular network profiling of U373MG human glioblastoma cells following induction of apoptosis by novel marine-derived anti-cancer 1,2,3,4-tetrahydroisoquinoline alkaloids. Cancer Cell Int. 2012 Apr 11;12(1):14. [Content Brief]

[2]. Saktrakulkla P, et al. Chemistry of ecteinascidins. Part 3: preparation of 2'-N-acyl derivatives of ecteinascidin 770 and evaluation of cytotoxicity. Bioorg Med Chem. 2011 Aug 1;19(15):4421-36. [Content Brief]

[3]. Powan P, et al. Ecteinascidin 770, a tetrahydroisoquinoline alkaloid, sensitizes human lung cancer cells to anoikis. Anticancer Res. 2013 Feb;33(2):505-12. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2973 mL	6.4863 mL	12.9727 mL	32.4317 mL
	5 mM	0.2595 mL	1.2973 mL	2.5945 mL	6.4863 mL
	10 mM	0.1297 mL	0.6486 mL	1.2973 mL	3.2432 mL
	15 mM	0.0865 mL	0.4324 mL	0.8648 mL	2.1621 mL
	20 mM	0.0649 mL	0.3243 mL	0.6486 mL	1.6216 mL
	25 mM	0.0519 mL	0.2595 mL	0.5189 mL	1.2973 mL
	30 mM	0.0432 mL	0.2162 mL	0.4324 mL	1.0811 mL
	40 mM	0.0324 mL	0.1622 mL	0.3243 mL	0.8108 mL
	50 mM	0.0259 mL	0.1297 mL	0.2595 mL	0.6486 mL
	60 mM	0.0216 mL	0.1081 mL	0.2162 mL	0.5405 mL

Ecteinascidin 770 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Ecteinascidin 770 (Synonyms: Ecteinascidine 770; Et-770)

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Ecteinascidin 770 Related Antibodies

2 Publications Citing Use of MCE Ecteinascidin 770

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Ecteinascidin 770 Related Classifications

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