FLI-06

Name: FLI-06
Brand: MedChemExpress
SKU: HY-15860
Rating: 5.0 (11 reviews)

Cat. No.: HY-15860 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

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FLI-06 is an inhibitor of Notch signaling with an EC₅₀ of 2.3 μM.

For research use only. We do not sell to patients.

FLI-06 Chemical Structure

CAS No. : 313967-18-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 97	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 97	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 88	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 132	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 440	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 770	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 11 publication(s) in Google Scholar

9 Publications Citing Use of MCE FLI-06

: FLI-06 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2018 Nov;107:1370-1376. [Abstract]; ECa109 and EC9706 cells are treated with different concentrations of FLI-06 (0, 10 and 20 μM) for 48 h, respectively. The expressions of LSD1 and H3K4me2 are investigated by Western blotting.

: FLI-06 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2018 Nov;107:1370-1376. [Abstract]; ECa109 and EC9706 cells are treated with different concentrations of FLI-06 (0, 10 and 20 μM) for 48 h, respectively. The expressions of Notch3, DTX1 and Hes1 are investigated by Western blotting.

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Protocol
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Description

FLI-06 is an inhibitor of Notch signaling with an EC₅₀ of 2.3 μM.

IC₅₀ & Target

EC50: 2.3 μM (Notch signaling)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	1.8 μM Compound: 1; FLI-06	Antiproliferative activity against human HCT116 cells after 72 hrs by SYBR green dye based fluorescence assay Antiproliferative activity against human HCT116 cells after 72 hrs by SYBR green dye based fluorescence assay	[PMID: 30201292]
HeLa	IC₅₀	2.3 μM Compound: 6; FLI-06	Inhibition of gamma secretase mediated Notch delta E activation in human HeLa cells expressing EGFP after 16 hrs by immunofluorescence analysis Inhibition of gamma secretase mediated Notch delta E activation in human HeLa cells expressing EGFP after 16 hrs by immunofluorescence analysis	[PMID: 27045975]

In Vitro

FLI-06, disrupted the Golgi apparatus in a manner distinct from that of brefeldin A and golgicide A. FLI-06 inhibited general secretion at a step before exit from the endoplasmic reticulum. In FLI-06–treated cells, no APPCTF accumulates despite strongly reduced Aβ secretion, suggesting that it acts upstream of α-secretase and β-secretase cleavage. FLI-06 is a very useful chemical probe to study the inhibition of membrane traffic at pre- ER-exit site (ERES) stages without fusion of ER-Golgi^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

438.52

Formula

C₂₅H₃₀N₂O₅

CAS No.

313967-18-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(C)NC2=C(C(CC(C)(C)C2)=O)C1C3=CC=C([N+]([O-])=O)C=C3)OC4CCCCC4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 38 mg/mL (86.66 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2804 mL	11.4020 mL	22.8040 mL
5 mM	0.4561 mL	2.2804 mL	4.5608 mL
10 mM	0.2280 mL	1.1402 mL	2.2804 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.70 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (252 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Kramer A, et al. Small molecules intercept Notch signaling and the early secretory pathway. Nat Chem Biol. 2013 Nov;9(11):731-8. [Content Brief]

Kinase Assay
^[1]

EC₅₀ values of the test compounds are calculated from serial dilution series ranging from 200 to 0.1 μM. Cells are seeded in 96-well plates at a density of 5,000 cells per well in 100 μL medium. The next day, 100 μL medium containing each test compound is added. Cells are incubated for 16 h, fixed and processed for automated microscopy. EC₅₀ estimates are calculated using four-parameter log-logistic fit with the package drc^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Kramer A, et al. Small molecules intercept Notch signaling and the early secretory pathway. Nat Chem Biol. 2013 Nov;9(11):731-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2804 mL	11.4020 mL	22.8040 mL	57.0099 mL
	5 mM	0.4561 mL	2.2804 mL	4.5608 mL	11.4020 mL
	10 mM	0.2280 mL	1.1402 mL	2.2804 mL	5.7010 mL
	15 mM	0.1520 mL	0.7601 mL	1.5203 mL	3.8007 mL
	20 mM	0.1140 mL	0.5701 mL	1.1402 mL	2.8505 mL
	25 mM	0.0912 mL	0.4561 mL	0.9122 mL	2.2804 mL
	30 mM	0.0760 mL	0.3801 mL	0.7601 mL	1.9003 mL
	40 mM	0.0570 mL	0.2850 mL	0.5701 mL	1.4252 mL
	50 mM	0.0456 mL	0.2280 mL	0.4561 mL	1.1402 mL
	60 mM	0.0380 mL	0.1900 mL	0.3801 mL	0.9502 mL
	80 mM	0.0285 mL	0.1425 mL	0.2850 mL	0.7126 mL

FLI-06 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FLI-06313967-18-9FLI06FLI 06NotchInhibitorinhibitorinhibit

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FLI-06

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FLI-06 Related Antibodies

9 Publications Citing Use of MCE FLI-06

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