Gboxin

Name: Gboxin
Brand: MedChemExpress
SKU: HY-111651
Rating: 5.0 (3 reviews)

Cat. No.: HY-111651 Purity: 98.23%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Gboxin is an oxidative phosphorylation (OXPHOS) inhibitor that targets glioblastoma. Gboxin inhibits the activity of F₀F₁ ATP synthase. Antitumour activity.

For research use only. We do not sell to patients.

Gboxin Chemical Structure

CAS No. : 2101315-36-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 116	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 116	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 105	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 330	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 820	In-stock	Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Gboxin

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Biological Activity
Purity & Documentation
References
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Description

Gboxin is an oxidative phosphorylation (OXPHOS) inhibitor that targets glioblastoma. Gboxin inhibits the activity of F₀F₁ ATP synthase. Antitumour activity^[1].

IC₅₀ & Target

F₀F₁ ATP synthase^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
GBM	IC₅₀	1 μM Compound: Gboxin	Growth inhibition of patient derived GBM cells incubated for 4 days by Celltiter glo assay Growth inhibition of patient derived GBM cells incubated for 4 days by Celltiter glo assay	[PMID: 33103432]
GBM	IC₅₀	150 nM Compound: Gboxin	Anticancer activity against human GBM cells carrying high-throughput GBM sphere assessed as reduction in cell viability incubated for 96 hrs by Cell-Titer-Glo assay Anticancer activity against human GBM cells carrying high-throughput GBM sphere assessed as reduction in cell viability incubated for 96 hrs by Cell-Titer-Glo assay	[PMID: 32942072]

In Vitro

Gboxin (0-15 μM; 96 hours) specifically inhibit the growth of primary ‘high-throughput GBM sphere’ (HTS) cells but not that of cycling primary low-passage mouse embryonic fibroblasts (MEFs) or astrocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HTS cells, MEFs and astrocytes
Concentration:	0, 185, 555, 1667, 5000, 15000 nM
Incubation Time:	96 hours
Result:	Inhibited the growth of HTS cells (IC₅₀=150 nM).

Molecular Weight

392.96

Formula

C₂₂H₃₃ClN₂O₂

CAS No.

2101315-36-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(O[C@H]1[C@H](C(C)C)CC[C@@H](C)C1)CN2C(CC)=[N+](C)C3=CC=CC=C23.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 32.5 mg/mL (82.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 10 mg/mL (25.45 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5448 mL	12.7239 mL	25.4479 mL
5 mM	0.5090 mL	2.5448 mL	5.0896 mL
10 mM	0.2545 mL	1.2724 mL	2.5448 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (5.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.17 mg/mL (5.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.17 mg/mL (5.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 2 mg/mL (5.09 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.23%

Select Batch:

Data Sheet (279 KB) SDS (251 KB)

COA (257 KB) RP-HPLC (281 KB)

Handling Instructions (2659 KB)

References

[1]. Shi Y, et al. Gboxin is an oxidative phosphorylation inhibitor that targets glioblastoma. Nature. 2019 Mar;567(7748):341-346. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.5448 mL	12.7239 mL	25.4479 mL	63.6197 mL
	5 mM	0.5090 mL	2.5448 mL	5.0896 mL	12.7239 mL
	10 mM	0.2545 mL	1.2724 mL	2.5448 mL	6.3620 mL
	15 mM	0.1697 mL	0.8483 mL	1.6965 mL	4.2413 mL
	20 mM	0.1272 mL	0.6362 mL	1.2724 mL	3.1810 mL
	25 mM	0.1018 mL	0.5090 mL	1.0179 mL	2.5448 mL
DMSO	30 mM	0.0848 mL	0.4241 mL	0.8483 mL	2.1207 mL
	40 mM	0.0636 mL	0.3181 mL	0.6362 mL	1.5905 mL
	50 mM	0.0509 mL	0.2545 mL	0.5090 mL	1.2724 mL
	60 mM	0.0424 mL	0.2121 mL	0.4241 mL	1.0603 mL
	80 mM	0.0318 mL	0.1590 mL	0.3181 mL	0.7952 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Gboxin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gboxin2101315-36-8Oxidative PhosphorylationATP SynthaseMitochondrial MetabolismInhibitorinhibitorinhibit

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Gboxin

Customer Review

Gboxin Related Antibodies

3 Publications Citing Use of MCE Gboxin

Featured Recommendations

•Related Screening Libraries:

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Purity & Documentation

References

Customer Review

Gboxin Related Antibodies

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Complete Stock Solution Preparation Table

Gboxin Related Classifications

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