Glutaminase C-IN-1 (Synonyms: Compound 968)

Name: Glutaminase C-IN-1
Brand: MedChemExpress
SKU: HY-12682
Rating: 5.0 (6 reviews)

Cat. No.: HY-12682 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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Glutaminase C-IN-1 (Compound 968) is an allosteric inhibitor of Glutaminase C that inhibits cancer cell growth without affecting their normal cellular counterparts.

For research use only. We do not sell to patients.

Glutaminase C-IN-1 Chemical Structure

CAS No. : 311795-38-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 77	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 77	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 495	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 6 publication(s) in Google Scholar

5 Publications Citing Use of MCE Glutaminase C-IN-1

Proliferation Assay

: Glutaminase C-IN-1 purchased from MedChemExpress. Usage Cited in: J Hepatol. 2020 May;72(5):909-923. [Abstract]; Proliferation of HCC-LM3 cells treated with Ctrl, 5 μM 968 alone, 4 μM Sor alone, and combined 5 μM 968 and 4 μM Sor (968+Sor).

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Description

Glutaminase C-IN-1 (Compound 968) is an allosteric inhibitor of Glutaminase C that inhibits cancer cell growth without affecting their normal cellular counterparts^[1]^[2].

IC₅₀ & Target

Glutaminase C^[2]

In Vitro

Glutaminase C-IN-1 (Compound 968) (10 μM; 14 d) inhibits cellular transformation in NIH 3T3 cells^[1].
Glutaminase C-IN-1 (10 μM; 6 d) blocks the signaling activity of a specific target of Dbl^[1].
Glutaminase C-IN-1 blocks the transforming activity of human breast cancer cells^[1].
Glutaminase C-IN-1 (10 μM) blocks glutaminolysis in transformed cells^[2].
Glutaminase C-IN-1 preferentially binds to the monomeric state of Glutaminase C^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MDA-MB231 cells, SKBR3 cells, and NIH 3T3 cells
Concentration:	10 μM
Incubation Time:	5 h
Result:	Inhibited cell growth.

In Vivo

Glutaminase C-IN-1 (Compound 968) (200 μg/mouse; i.p.; daily for 12 days) reduces tumor volume in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SCID mice with P493 B lymphoma cells^[1]
Dosage:	200 μg/mouse
Administration:	Intraperitoneal injection, daily for 12 days
Result:	Caused a ~50% reduction in the size of the tumors.

Molecular Weight

475.42

Formula

C₂₇H₂₇BrN₂O

CAS No.

311795-38-7

Appearance

Solid

Color

White to off-white

SMILES

O=C1C(C(C2=CC=C(N(C)C)C(Br)=C2)NC3=C4C5=CC=CC=C5C=C3)=C4CC(C)(C)C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (52.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1034 mL	10.5170 mL	21.0340 mL
5 mM	0.4207 mL	2.1034 mL	4.2068 mL
10 mM	0.2103 mL	1.0517 mL	2.1034 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.26 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (278 KB) SDS (394 KB)

COA (253 KB) HNMR (263 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Wang JB, et al. Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Cancer Cell. 2010 Sep 14;18(3):207-19. [Content Brief]

[2]. Stalnecker CA, et al. Mechanism by which a recently discovered allosteric inhibitor blocks glutamine metabolism in transformed cells. Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):394-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1034 mL	10.5170 mL	21.0340 mL	52.5851 mL
	5 mM	0.4207 mL	2.1034 mL	4.2068 mL	10.5170 mL
	10 mM	0.2103 mL	1.0517 mL	2.1034 mL	5.2585 mL
	15 mM	0.1402 mL	0.7011 mL	1.4023 mL	3.5057 mL
	20 mM	0.1052 mL	0.5259 mL	1.0517 mL	2.6293 mL
	25 mM	0.0841 mL	0.4207 mL	0.8414 mL	2.1034 mL
	30 mM	0.0701 mL	0.3506 mL	0.7011 mL	1.7528 mL
	40 mM	0.0526 mL	0.2629 mL	0.5259 mL	1.3146 mL
	50 mM	0.0421 mL	0.2103 mL	0.4207 mL	1.0517 mL

Glutaminase C-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Glutaminase C-IN-1311795-38-7Compound 968Compound968Compound-968GlutaminaseGlutaminase CcancerNIH 3T3glutaminolysisSKBR3MDA-MB231P493 B lymphomaInhibitorinhibitorinhibit

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Glutaminase C-IN-1 (Synonyms: Compound 968)

Customer Review

Glutaminase C-IN-1 Related Antibodies

5 Publications Citing Use of MCE Glutaminase C-IN-1

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•Related Screening Libraries:

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Biological Activity

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References

Customer Review

Glutaminase C-IN-1 Related Antibodies

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Complete Stock Solution Preparation Table

Glutaminase C-IN-1 Related Classifications

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