2. Membrane Transporter/Ion Channel
  3. CFTR
  4. GlyH-101

GlyH-101 is a potent CFTR inhibitor. GlyH-101 also is a potent and reversible inhibitor of the VSORC conductance. GlyH-101 shows antiproliferative activity. GlyH-101 inhibits CFTR-like current and VSORC current.

For research use only. We do not sell to patients.

GlyH-101 Chemical Structure

GlyH-101 Chemical Structure

CAS No. : 328541-79-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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 USD 106 In-stock
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10 mM * 1 mL in DMSO USD 106 In-stock
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Customer Review

Based on 4 publication(s) in Google Scholar

GlyH-101 Related Antibodies

Top Publications Citing Use of Products

    GlyH-101 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2018 Apr 16;8(1):6028.  [Abstract]

    Western blot analysis of expression and phosphorylation of H3 in GlyH-101 (10 nM for 48 hours)-treated cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    GlyH-101 is a potent CFTR inhibitor. GlyH-101 also is a potent and reversible inhibitor of the VSORC conductance. GlyH-101 shows antiproliferative activity. GlyH-101 inhibits CFTR-like current and VSORC current[1][2].

    In Vitro

    GlyH-101 (0-50 μM) shows antiproliferative activity in PCT and PS120 cells[1].
    GlyH-101 (0.5, 1, 5, 10 μM) inhibits CFTR-like current in a concentration-dependent manner in PCT cells[1].
    GlyH-101 (0.5, 1, 5, 10 μM) inhibits the VSORC current with IC50s of 5.38, 6.26 μM for PS120, PCT cells, respectively[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    GlyH-101 Related Antibodies

    Cell Viability Assay[1]

    Cell Line: PCT, PS120 cells
    Concentration: 0, 1, 5, 10, 20, 50 µM
    Incubation Time: 24 h
    Result: Inhibited the cell growth in a dose-dependent manner.
    In Vivo

    GlyH-101 (2.5 μg) reduces by about 80% cholera toxin-induced intestinal fluid secretion in mouse[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    493.15

    Formula

    C19H15Br2N3O3
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(N/N=C/C1=CC(Br)=C(O)C(Br)=C1O)CNC2=CC3=CC=CC=C3C=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 58 mg/mL (117.61 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0278 mL 10.1389 mL 20.2778 mL
    5 mM 0.4056 mL 2.0278 mL 4.0556 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

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    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.07 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.68%

    COA (246 KB) LCMS (104 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0278 mL 10.1389 mL 20.2778 mL 50.6945 mL
    5 mM 0.4056 mL 2.0278 mL 4.0556 mL 10.1389 mL
    10 mM 0.2028 mL 1.0139 mL 2.0278 mL 5.0695 mL
    15 mM 0.1352 mL 0.6759 mL 1.3519 mL 3.3796 mL
    20 mM 0.1014 mL 0.5069 mL 1.0139 mL 2.5347 mL
    25 mM 0.0811 mL 0.4056 mL 0.8111 mL 2.0278 mL
    30 mM 0.0676 mL 0.3380 mL 0.6759 mL 1.6898 mL
    40 mM 0.0507 mL 0.2535 mL 0.5069 mL 1.2674 mL
    50 mM 0.0406 mL 0.2028 mL 0.4056 mL 1.0139 mL
    60 mM 0.0338 mL 0.1690 mL 0.3380 mL 0.8449 mL
    80 mM 0.0253 mL 0.1267 mL 0.2535 mL 0.6337 mL
    100 mM 0.0203 mL 0.1014 mL 0.2028 mL 0.5069 mL
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    GlyH-101 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    GlyH-101328541-79-3GlyH101GlyH 101CFTRCystic fibrosis transmembrane conductance regulatorantiproliferativePCTPS120 cellsVSORCCFTR-like currentintestinal fluidInhibitorinhibitorinhibit

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    Cat. No.:
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