2. Membrane Transporter/Ion Channel PI3K/Akt/mTOR Stem Cell/Wnt MAPK/ERK Pathway
  3. Piezo Channel Akt ERK Potassium Channel
  4. Yoda 1

Yoda 1 is a potent and selective Piezo1 agonist. Yoda 1 activates purified Piezo1 channels. Yoda 1 potently inhibits macropinocytosis induced by epidermal growth factor (EGF). Yoda 1 enhances Ca2+ influx followed by activation of the calcium-activated potassium channel KCa3.1 and inhibition of Rac1 activation.

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Yoda 1 Chemical Structure

Yoda 1 Chemical Structure

CAS No. : 448947-81-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 118 In-stock
Solution
10 mM * 1 mL in DMSO USD 118 In-stock
Solid
1 mg USD 51 In-stock
5 mg USD 108 In-stock
10 mg USD 144 In-stock
50 mg USD 576 In-stock
100 mg USD 1020 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 37 publication(s) in Google Scholar

Yoda 1 Related Antibodies

Top Publications Citing Use of Products

37 Publications Citing Use of MCE Yoda 1

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

View All ERK Isoform Specific Products:

View All Isoforms
ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Yoda 1 is a potent and selective Piezo1 agonist. Yoda 1 activates purified Piezo1 channels. Yoda 1 potently inhibits macropinocytosis induced by epidermal growth factor (EGF). Yoda 1 enhances Ca2+ influx followed by activation of the calcium-activated potassium channel KCa3.1 and inhibition of Rac1 activation[1][2][3].

IC50 & Target

ERK1

 

ERK2

 

In Vitro

Yoda1 (0-6 μM, 5 min) induces the activation of both Akt and ERK1/2, which is not dependent on Piezo1[2].
Yoda1 (1.5 μM, 5 min) inhibits Rac1 activation[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Yoda 1 Related Antibodies

Western Blot Analysis

Cell Line: human coronary artery endothelial cells (HCAECs)[2], A431 cells[3]
Concentration: 0, 1.5, 3.0, and 6.0 μM
Incubation Time: 5 min
Result: Induced the activation of both Akt and ERK1/2, and increased the phosphorylation levels of both Akt and ERK1/2 in a dose-dependent manner. Inhibited EGF-induced increase in the amount of Rac1-GTP, and inhibited Rac1 activation
Molecular Weight

355.27

Formula

C13H8Cl2N4S2
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

ClC1=C(CSC2=NN=C(C3=NC=CN=C3)S2)C(Cl)=CC=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

DMSO : 15.62 mg/mL (43.97 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 5 mg/mL (14.07 mM; Need ultrasonic)

Methanol : 2.5 mg/mL (7.04 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8148 mL 14.0738 mL 28.1476 mL
5 mM 0.5630 mL 2.8148 mL 5.6295 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.56 mg/mL (4.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.56 mg/mL (4.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  15% Cremophor EL    85% Saline

    Solubility: 0.62 mg/mL (1.75 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.98%

COA (250 KB) RP-HPLC (252 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Methanol / Ethanol / DMSO 1 mM 2.8148 mL 14.0738 mL 28.1476 mL 70.3690 mL
5 mM 0.5630 mL 2.8148 mL 5.6295 mL 14.0738 mL
Ethanol / DMSO 10 mM 0.2815 mL 1.4074 mL 2.8148 mL 7.0369 mL
DMSO 15 mM 0.1877 mL 0.9383 mL 1.8765 mL 4.6913 mL
20 mM 0.1407 mL 0.7037 mL 1.4074 mL 3.5185 mL
25 mM 0.1126 mL 0.5630 mL 1.1259 mL 2.8148 mL
30 mM 0.0938 mL 0.4691 mL 0.9383 mL 2.3456 mL
40 mM 0.0704 mL 0.3518 mL 0.7037 mL 1.7592 mL
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Yoda 1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Yoda 1448947-81-7Yoda1Yoda-1Piezo ChannelAktERKPotassium ChannelPKBProtein kinase BExtracellular signal regulated kinasesKcsAA431 cellsHCAECsPiezo1Endothelial cellsERK1/2Inhibitorinhibitorinhibit

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