JJKK 048

Name: JJKK 048
Brand: MedChemExpress
SKU: HY-108613
Rating: 4.5 (1 reviews)

Cat. No.: HY-108613 Purity: 99.55%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

JJKK 048 is an ultrapotent and highly selective inhibitor of Monoacylglycerol lipase (MAGL).

For research use only. We do not sell to patients.

JJKK 048 Chemical Structure

CAS No. : 1515855-97-6

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 99	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 99	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 490	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 780	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE JJKK 048

•J Pharm Sci. 2023 May 21;S0022-3549(23)00198-3. [Abstract]

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References
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Description

JJKK 048 is an ultrapotent and highly selective inhibitor of Monoacylglycerol lipase (MAGL).

IC₅₀ & Target

MAGL^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	IC₅₀	4800 nM Compound: JJKK-048	Inhibition of human recombinant FAAH expressed in COS7 cells using anandamide as substrate Inhibition of human recombinant FAAH expressed in COS7 cells using anandamide as substrate	[PMID: 25282655]
HEK293	IC₅₀	0.4 nM Compound: JJKK-048	Inhibition of human recombinant MAGL expressed in HEK293 cells using 2-AG as substrate Inhibition of human recombinant MAGL expressed in HEK293 cells using 2-AG as substrate	[PMID: 25282655]

Molecular Weight

434.44

Formula

C₂₃H₂₂N₄O₅

CAS No.

1515855-97-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCC(C(C2=CC=C(OCO3)C3=C2)C4=CC=C(OCO5)C5=C4)CC1)N6N=CN=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (76.72 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3018 mL	11.5091 mL	23.0181 mL
5 mM	0.4604 mL	2.3018 mL	4.6036 mL
10 mM	0.2302 mL	1.1509 mL	2.3018 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.55%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (257 KB) RP-HPLC (208 KB) MS (181 KB)

Handling Instructions (2659 KB)

References

[1]. Aaltonen N, et al. In Vivo Characterization of the Ultrapotent Monoacylglycerol Lipase Inhibitor {4-[bis-(benzo[d][1,3]dioxol-5-yl)methyl]-piperidin-1-yl}(1H-1,2,4-triazol-1-yl)methanone (JJKK-048). J Pharmacol Exp Ther. 2016 Oct;359(1):62-72. [Content Brief]

[2]. Aaltonen N, et al. Piperazine and piperidine triazole ureas as ultrapotent and highly selective inhibitors of monoacylglycerol lipase. Chem Biol. 2013 Mar 21;20(3):379-90. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3018 mL	11.5091 mL	23.0181 mL	57.5453 mL
	5 mM	0.4604 mL	2.3018 mL	4.6036 mL	11.5091 mL
	10 mM	0.2302 mL	1.1509 mL	2.3018 mL	5.7545 mL
	15 mM	0.1535 mL	0.7673 mL	1.5345 mL	3.8364 mL
	20 mM	0.1151 mL	0.5755 mL	1.1509 mL	2.8773 mL
	25 mM	0.0921 mL	0.4604 mL	0.9207 mL	2.3018 mL
	30 mM	0.0767 mL	0.3836 mL	0.7673 mL	1.9182 mL
	40 mM	0.0575 mL	0.2877 mL	0.5755 mL	1.4386 mL
	50 mM	0.0460 mL	0.2302 mL	0.4604 mL	1.1509 mL
	60 mM	0.0384 mL	0.1918 mL	0.3836 mL	0.9591 mL

JJKK 048 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JJKK 0481515855-97-6JJKK048JJKK-048MAGLMonoacylglycerol lipaseInhibitorinhibitorinhibit

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JJKK 048

Customer Review

JJKK 048 Related Antibodies

1 Publications Citing Use of MCE JJKK 048

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

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References

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Complete Stock Solution Preparation Table

JJKK 048 Related Classifications

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