2. Metabolic Enzyme/Protease PI3K/Akt/mTOR Apoptosis
  3. Endogenous Metabolite mTOR Apoptosis
  4. L-Proline

L-Proline is one of the twenty amino acids used in living organisms as the building blocks of proteins.

For research use only. We do not sell to patients.

L-Proline Chemical Structure

L-Proline Chemical Structure

CAS No. : 147-85-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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10 mM * 1 mL in Water In-stock
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Based on 4 publication(s) in Google Scholar

Other Forms of L-Proline:

L-Proline Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

L-Proline is one of the twenty amino acids used in living organisms as the building blocks of proteins.

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

L-Proline (1 g/L, 48 h) inhibits AFB1- or AFM1-induced apoptosis through downregulation of PRODH expression and its downstream apoptotic factors (Bax, Bcl-2 and cleaved Caspase-3)[1].
L-Proline (40-400 μM, 4 days) activates the mTOR signaling pathway, induces the differentiation of mouse embryonic stem cells (ES cells)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Proline Related Antibodies

Apoptosis Analysis[1]

Cell Line: HEK293
Concentration: 1 g/L
Incubation Time: 48 h
Result: Inhibited AFB1- or AFM1-induced apoptosis.
In Vivo

L-Proline (10 g/kg, po for 28 days) exhibits protective effect against renal oxidative damage and dysfunction caused by AFB1 and AFM1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CD-1 mouse AFB1 and AFM1 induced kidney damage model[1]
Dosage: 10 g/kg
Administration: po for 28 days
Result: Ameliorated AFB1 and AFM1 induced renal oxidative damage and dysfunction.
Molecular Weight

115.13

Formula

C5H9NO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@H]1NCCC1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

RT, protect from light, stored under nitrogen

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (434.28 mM; Need ultrasonic)

DMSO : 2 mg/mL (17.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.6857 mL 43.4284 mL 86.8568 mL
5 mM 1.7371 mL 8.6857 mL 17.3714 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (868.57 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (393 KB)

COA (249 KB) HNMR (234 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 8.6857 mL 43.4284 mL 86.8568 mL 217.1421 mL
5 mM 1.7371 mL 8.6857 mL 17.3714 mL 43.4284 mL
10 mM 0.8686 mL 4.3428 mL 8.6857 mL 21.7142 mL
15 mM 0.5790 mL 2.8952 mL 5.7905 mL 14.4761 mL
H2O 20 mM 0.4343 mL 2.1714 mL 4.3428 mL 10.8571 mL
25 mM 0.3474 mL 1.7371 mL 3.4743 mL 8.6857 mL
30 mM 0.2895 mL 1.4476 mL 2.8952 mL 7.2381 mL
40 mM 0.2171 mL 1.0857 mL 2.1714 mL 5.4286 mL
50 mM 0.1737 mL 0.8686 mL 1.7371 mL 4.3428 mL
60 mM 0.1448 mL 0.7238 mL 1.4476 mL 3.6190 mL
80 mM 0.1086 mL 0.5429 mL 1.0857 mL 2.7143 mL
100 mM 0.0869 mL 0.4343 mL 0.8686 mL 2.1714 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Proline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Proline147-85-3Endogenous MetabolitemTORApoptosisMammalian target of RapamycinInhibitorinhibitorinhibit

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