2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. L189

L189 is a DNA ligase inhibitor. L189 has inhibition effect for DNA Ligase I, III and IV with IC50 values of 5 μM, 9 μM and 5 μM, respectively. L189 has no cytotoxicity and individually increase cell death. L189 can be used for the research of cancer.

For research use only. We do not sell to patients.

L189 Chemical Structure

L189 Chemical Structure

CAS No. : 64232-83-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 81 In-stock
Solution
10 mM * 1 mL in DMSO USD 81 In-stock
Solid
5 mg USD 74 In-stock
10 mg USD 119 In-stock
25 mg USD 245 In-stock
50 mg USD 428 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

L189 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

L189 is a DNA ligase inhibitor. L189 has inhibition effect for DNA Ligase I, III and IV with IC50 values of 5 μM, 9 μM and 5 μM, respectively. L189 has no cytotoxicity and individually increase cell death. L189 can be used for the research of cancer[1][2].

In Vitro

L189 inhibits DNA Ligase I, III and IV activity with IC50 values of 5 μM, 9 μM and 5 μM, respectively[1].
L189 (5 μM, 48 h) bring good anti-proliferation activity preferentially and cause cell death by creating a cytotoxic environment[2].
L189 (5 μM, 48 h) reduces HeLa nuclear staining along with TMZ[2].
L189 (5 μM, 48 h) enhances TMZ-induced HeLa growth arrest when along with TMZ that possibly in G2/M cell cycle phase without employing cell death mechanisms[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L189 Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: HeLa cells
Concentration: 5 μM
Incubation Time: 48 h
Result: Blocked to HeLa growth and proliferation along with TMZ and not mark the significant cell cytotoxicity alone.
Molecular Weight

246.29

Formula

C11H10N4OS
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=NC(S)=NC(N)=C1/N=C/C2=CC=CC=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 33 mg/mL (133.99 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0603 mL 20.3013 mL 40.6025 mL
5 mM 0.8121 mL 4.0603 mL 8.1205 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.0603 mL 20.3013 mL 40.6025 mL 101.5064 mL
5 mM 0.8121 mL 4.0603 mL 8.1205 mL 20.3013 mL
10 mM 0.4060 mL 2.0301 mL 4.0603 mL 10.1506 mL
15 mM 0.2707 mL 1.3534 mL 2.7068 mL 6.7671 mL
20 mM 0.2030 mL 1.0151 mL 2.0301 mL 5.0753 mL
25 mM 0.1624 mL 0.8121 mL 1.6241 mL 4.0603 mL
30 mM 0.1353 mL 0.6767 mL 1.3534 mL 3.3835 mL
40 mM 0.1015 mL 0.5075 mL 1.0151 mL 2.5377 mL
50 mM 0.0812 mL 0.4060 mL 0.8121 mL 2.0301 mL
60 mM 0.0677 mL 0.3384 mL 0.6767 mL 1.6918 mL
80 mM 0.0508 mL 0.2538 mL 0.5075 mL 1.2688 mL
100 mM 0.0406 mL 0.2030 mL 0.4060 mL 1.0151 mL
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L189 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L18964232-83-3L 189L-189DNA/RNA SynthesisDNA ligaseDNA Ligase IDNA Ligase IIIDNA Ligase IVcytotoxicitycell deathTMZcancerInhibitorinhibitorinhibit

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