2. Cell Cycle/DNA Damage Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation NF-κB
  3. DNA/RNA Synthesis Caspase Apoptosis Reactive Oxygen Species
  4. L67

L67  (Synonyms: DNA Ligase Inhibitor)

Cat. No.: HY-15586 Purity: ≥98.0%
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L67 (DNA Ligase Inhibitor) is a competitive DNA ligase inhibitor that effectively inhibits DNA ligases I/III (both IC50 are 10 μM). L67 can cause nuclear DNA damage by reducing levels of mitochondrial DNA and increasing levels of mitochondrially-generated ROS. L67 also activates the Caspase 1-dependent apoptosis pathway in cancer cells, can be used in cancer research.

For research use only. We do not sell to patients.

L67 Chemical Structure

L67 Chemical Structure

CAS No. : 325970-71-6

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Based on 1 publication(s) in Google Scholar

L67 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE L67

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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Description

L67 (DNA Ligase Inhibitor) is a competitive DNA ligase inhibitor that effectively inhibits DNA ligases I/III (both IC50 are 10 μM). L67 can cause nuclear DNA damage by reducing levels of mitochondrial DNA and increasing levels of mitochondrially-generated ROS. L67 also activates the Caspase 1-dependent apoptosis pathway in cancer cells, can be used in cancer research[1][2].

IC50 & Target[1][2]

DNA ligases I

10 μM (IC50)

DNA ligases III

10 μM (IC50)

Caspase-1

 

In Vitro

L67 (10, 15 μM; 24 h) promotes nuclear DNA damage and (0-50 μM) increases the level of mSOX by inhibiting mitochondrial LigIIIα in HeLa cells[1].
L67 (10 μM; 24 h) induces changes in mitochondrial function that cause a reduction in OCR and mitochondrial DNA, and abnormal mitochondrial morphology in HeLa[1].
L67 (10, 100 μM; 24 h) induces apoptosis in cancer cells[1].
L67 (0-30 μM; 24 h) selectively induces cell death in cancer cells by activating a caspase 1-dependent apoptotic pathway[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L67 Related Antibodies

Cell Viability Assay[1]

Cell Line: HeLa cells
Concentration: 10, 15 μM; 0-50 μM
Incubation Time: 24 h
Result: Increased the formation of nuclear γH2AX foci and steady state levels of γH2AX when at 10 or 15 μM. (γH2AX: a sign of DNA double-strand breaks).
Resulted in a concentration (0-50 μM)-dependent increase in mSOX (mitochondrial superoxide) levels. (mSOX is a major cause of the cellular oxidative damage).

Cell Viability Assay[1]

Cell Line: HeLa cells
Concentration: 10 μM
Incubation Time: 24 h
Result: Reduced oxygen consumption rate (OCR) approximately 20%.
Resulted in about a 25% reduction in mitochondrial DNA.

Apoptosis Analysis[1]

Cell Line: HeLa cells
Concentration: 10, 100 μM
Incubation Time: 24 h
Result: Result:Induced apoptosis, and at 100 μM with apoptotic cells constituting about 50% of the HeLa cell population.

Cell Viability Assay[1]

Cell Line: HeLa cells
Concentration: 0-30 μM
Incubation Time: 24 h
Result: Activates a caspase 1-dependent cell death pathway in cancer cells.
Molecular Weight

486.11

Formula

C16H14Br2N4O4
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

BrC1=CC(NCC(N/N=C/C2=CC([N+]([O-])=O)=CC=C2O)=O)=CC(Br)=C1C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (205.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0571 mL 10.2857 mL 20.5715 mL
5 mM 0.4114 mL 2.0571 mL 4.1143 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0571 mL 10.2857 mL 20.5715 mL 51.4287 mL
5 mM 0.4114 mL 2.0571 mL 4.1143 mL 10.2857 mL
10 mM 0.2057 mL 1.0286 mL 2.0571 mL 5.1429 mL
15 mM 0.1371 mL 0.6857 mL 1.3714 mL 3.4286 mL
20 mM 0.1029 mL 0.5143 mL 1.0286 mL 2.5714 mL
25 mM 0.0823 mL 0.4114 mL 0.8229 mL 2.0571 mL
30 mM 0.0686 mL 0.3429 mL 0.6857 mL 1.7143 mL
40 mM 0.0514 mL 0.2571 mL 0.5143 mL 1.2857 mL
50 mM 0.0411 mL 0.2057 mL 0.4114 mL 1.0286 mL
60 mM 0.0343 mL 0.1714 mL 0.3429 mL 0.8571 mL
80 mM 0.0257 mL 0.1286 mL 0.2571 mL 0.6429 mL
100 mM 0.0206 mL 0.1029 mL 0.2057 mL 0.5143 mL
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L67 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L67325970-71-6DNA Ligase InhibitorL 67L-67DNA/RNA SynthesisCaspaseApoptosisReactive Oxygen SpeciesDNAmitochondrialDNA damageROSmSOXγH2AXHeLacaspase 1Inhibitorinhibitorinhibit

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