2. Metabolic Enzyme/Protease Apoptosis
  3. Proteasome Apoptosis
  4. MG-101

MG-101  (Synonyms: Calpain inhibitor I; Ac-LLnL-CHO; ALLN)

Cat. No.: HY-18964 Purity: ≥98.0%
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MG-101 (ALLN) is an inhibitor of cysteine proteases which inhibits calpain I, calpain II, cathepsin B and cathepsin L with Kis of 190, 220, 150 and 500 pM, respectively. MG-101 induces apoptosis and inhibits tumor growth, it can be used for the research of colon cancer.

For research use only. We do not sell to patients.

MG-101 Chemical Structure

MG-101 Chemical Structure

CAS No. : 110044-82-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
1 mg USD 30 In-stock
5 mg USD 60 In-stock
10 mg USD 90 In-stock
25 mg USD 190 In-stock
50 mg USD 300 In-stock
100 mg USD 480 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 10 publication(s) in Google Scholar

MG-101 Related Antibodies

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  • Purity & Documentation

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Description

MG-101 (ALLN) is an inhibitor of cysteine proteases which inhibits calpain I, calpain II, cathepsin B and cathepsin L with Kis of 190, 220, 150 and 500 pM, respectively. MG-101 induces apoptosis and inhibits tumor growth, it can be used for the research of colon cancer[1].

In Vitro

MG-101 (0-26 μM; 24 h) affects the growth of colon cancer HCT116 cells[1].
MG-101 (0-26 μM; 24 h) induces apoptosis of various cancer cells[1].
MG-101 (0-26 μM; 24 h) induces Bax-dependent apoptosis in HCT116 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MG-101 Related Antibodies

Cell Viability Assay[1]

Cell Line: HCT116 cell line
Concentration: 0-26 μM
Incubation Time: 24 hours
Result: Anchorage-independently and significantly decreased the viability of HCT116 cells.

Western Blot Analysis[1]

Cell Line: HCT116, RKO, SW480 and HepG2 cell lines
Concentration: 0-26 μM
Incubation Time: 24 hours
Result: Dose-dependently decreased the concentration of pro-caspase 3 and increased the concentration of cleaved-PARP in HCT116 cells. Induced apoptosis of HCT116, RKO, SW480 and HepG2 cells.

Western Blot Analysis[1]

Cell Line: HCT116, HCT116/p53-/- and HCT116/Bax-/- cell lines
Concentration: 0-26 μM
Incubation Time: 24 hours
Result: Induced apoptosis via a Bax-dependent manner, but not via a p53-dependent manner.
In Vivo

MG-101 (10 mg/kg; i.p. once daily for 15 days) inhibits tumor growth in nude mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female athymic nude mice with HCT116 cells injection[1]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; 10 mg/kg once daily; for 15 days
Result: Significantly decreased tumor weight and volume of treated mice at day 26 compared to control mice, and showed no effect on body weights.
Molecular Weight

383.53

Formula

C20H37N3O4
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(N[C@H](C=O)CCCC)[C@@H](NC([C@@H](NC(C)=O)CC(C)C)=O)CC(C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (325.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6074 mL 13.0368 mL 26.0736 mL
5 mM 0.5215 mL 2.6074 mL 5.2147 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.67 mg/mL (4.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1.67 mg/mL (4.35 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (250 KB) HNMR (234 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6074 mL 13.0368 mL 26.0736 mL 65.1839 mL
5 mM 0.5215 mL 2.6074 mL 5.2147 mL 13.0368 mL
10 mM 0.2607 mL 1.3037 mL 2.6074 mL 6.5184 mL
15 mM 0.1738 mL 0.8691 mL 1.7382 mL 4.3456 mL
20 mM 0.1304 mL 0.6518 mL 1.3037 mL 3.2592 mL
25 mM 0.1043 mL 0.5215 mL 1.0429 mL 2.6074 mL
30 mM 0.0869 mL 0.4346 mL 0.8691 mL 2.1728 mL
40 mM 0.0652 mL 0.3259 mL 0.6518 mL 1.6296 mL
50 mM 0.0521 mL 0.2607 mL 0.5215 mL 1.3037 mL
60 mM 0.0435 mL 0.2173 mL 0.4346 mL 1.0864 mL
80 mM 0.0326 mL 0.1630 mL 0.3259 mL 0.8148 mL
100 mM 0.0261 mL 0.1304 mL 0.2607 mL 0.6518 mL
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MG-101 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MG-101110044-82-1Calpain inhibitor I Ac-LLnL-CHO ALLNMG101MG 101ProteasomeApoptosisanti-tumorcolon cancerapoptosisBaxmitochondriaHCT116RKOSW480HepG2Inhibitorinhibitorinhibit

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