MG-101 (Synonyms: Calpain inhibitor I; Ac-LLnL-CHO; ALLN)

Name: MG-101
Brand: MedChemExpress
SKU: HY-18964
Rating: 5.0 (10 reviews)

Cat. No.: HY-18964 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MG-101 (ALLN) is an inhibitor of cysteine proteases which inhibits calpain I, calpain II, cathepsin B and cathepsin L with K_is of 190, 220, 150 and 500 pM, respectively. MG-101 induces apoptosis and inhibits tumor growth, it can be used for the research of colon cancer.

For research use only. We do not sell to patients.

MG-101 Chemical Structure

CAS No. : 110044-82-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 10 publication(s) in Google Scholar

10 Publications Citing Use of MCE MG-101

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	CC₅₀	25.1 μM Compound: ALLN	Cytotoxicity against human HeLa cells assessed as growth inhibition after 48 hrs by MTS assay Cytotoxicity against human HeLa cells assessed as growth inhibition after 48 hrs by MTS assay	[PMID: 23357632]

In Vitro

MG-101 (0-26 μM; 24 h) affects the growth of colon cancer HCT116 cells^[1].
MG-101 (0-26 μM; 24 h) induces apoptosis of various cancer cells^[1].
MG-101 (0-26 μM; 24 h) induces Bax-dependent apoptosis in HCT116 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HCT116 cell line
Concentration:	0-26 μM
Incubation Time:	24 hours
Result:	Anchorage-independently and significantly decreased the viability of HCT116 cells.

Western Blot Analysis^[1]

Cell Line:	HCT116, RKO, SW480 and HepG2 cell lines
Concentration:	0-26 μM
Incubation Time:	24 hours
Result:	Dose-dependently decreased the concentration of pro-caspase 3 and increased the concentration of cleaved-PARP in HCT116 cells. Induced apoptosis of HCT116, RKO, SW480 and HepG2 cells.

Western Blot Analysis^[1]

Cell Line:	HCT116, HCT116/p53^-/- and HCT116/Bax^-/- cell lines
Concentration:	0-26 μM
Incubation Time:	24 hours
Result:	Induced apoptosis via a Bax-dependent manner, but not via a p53-dependent manner.

In Vivo

MG-101 (10 mg/kg; i.p. once daily for 15 days) inhibits tumor growth in nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female athymic nude mice with HCT116 cells injection^[1]
Dosage:	10 mg/kg
Administration:	Intraperitoneal injection; 10 mg/kg once daily; for 15 days
Result:	Significantly decreased tumor weight and volume of treated mice at day 26 compared to control mice, and showed no effect on body weights.

Molecular Weight

383.53

Formula

C₂₀H₃₇N₃O₄

CAS No.

110044-82-1

Appearance

Solid

Color

White to light yellow

SMILES

O=C(N[C@H](C=O)CCCC)[C@@H](NC([C@@H](NC(C)=O)CC(C)C)=O)CC(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (260.74 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6074 mL	13.0368 mL	26.0736 mL
5 mM	0.5215 mL	2.6074 mL	5.2147 mL
10 mM	0.2607 mL	1.3037 mL	2.6074 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (4.35 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.67 mg/mL (4.35 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (4.35 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (250 KB) HNMR (234 KB)

Handling Instructions (2659 KB)

References

[1]. Li SZ, et al. ALLN hinders HCT116 tumor growth through Bax-dependent apoptosis. Biochem Biophys Res Commun. 2013 Jul 26;437(2):325-330. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6074 mL	13.0368 mL	26.0736 mL	65.1839 mL
	5 mM	0.5215 mL	2.6074 mL	5.2147 mL	13.0368 mL
	10 mM	0.2607 mL	1.3037 mL	2.6074 mL	6.5184 mL
	15 mM	0.1738 mL	0.8691 mL	1.7382 mL	4.3456 mL
	20 mM	0.1304 mL	0.6518 mL	1.3037 mL	3.2592 mL
	25 mM	0.1043 mL	0.5215 mL	1.0429 mL	2.6074 mL
	30 mM	0.0869 mL	0.4346 mL	0.8691 mL	2.1728 mL
	40 mM	0.0652 mL	0.3259 mL	0.6518 mL	1.6296 mL
	50 mM	0.0521 mL	0.2607 mL	0.5215 mL	1.3037 mL
	60 mM	0.0435 mL	0.2173 mL	0.4346 mL	1.0864 mL
	80 mM	0.0326 mL	0.1630 mL	0.3259 mL	0.8148 mL
	100 mM	0.0261 mL	0.1304 mL	0.2607 mL	0.6518 mL

MG-101 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MG-101 (Synonyms: Calpain inhibitor I; Ac-LLnL-CHO; ALLN)

Customer Review

MG-101 Related Antibodies

10 Publications Citing Use of MCE MG-101

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

MG-101 Related Antibodies

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Complete Stock Solution Preparation Table

MG-101 Related Classifications

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