MLT-748

Name: MLT-748
Brand: MedChemExpress
SKU: HY-115466
Rating: 5.0 (4 reviews)

Cat. No.: HY-115466 Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MLT-748 is a potent, selective and allosteric inhibitor of MALT1, binds MALT1 in the allosteric Trp580 pocket, with an IC₅₀ of 5 nM.

For research use only. We do not sell to patients.

MLT-748 Chemical Structure

CAS No. : 1832578-30-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 325	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 325	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1480	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE MLT-748

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Description

MLT-748 is a potent, selective and allosteric inhibitor of MALT1, binds MALT1 in the allosteric Trp580 pocket, with an IC₅₀ of 5 nM^[1].

IC₅₀ & Target

IC50: 5 nM (MALT1)^[1]
Kd: 13 nM (MALT1(329-728)-W580S), 42 nM (MALT1(329-728))^[1]

In Vitro

MLT-748 reversibly binds to human mutant MALT1(329-728)-W580S (K_d, 13 nM) with affinity similar to that of the wild type MALT1(329-728) (K_d, 42 nM)^[1].
MLT-748 (0-2 μM) stabilizes cellular MALT1-W580S, with an EC₅₀ of 69 nM^[1].
MLT-748 (2 μM, 24 hours) increases the phosphorylation of p65 and IκBα in MALT1^mut/mut patient immortalized B cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	MALT1^mut/mut patient immortalized B cells
Concentration:	2 µM
Incubation Time:	24 hours
Result:	Increased NF-κB signaling phosphorylation of both p65 and IκBα as early as 5 min after stimulation with PMA and ionomycin.

Molecular Weight

492.32

Formula

C₁₉H₁₉Cl₂N₉O₃

CAS No.

1832578-30-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC(Cl)=C(N2N=CC=N2)N=C1)NC3=C([C@@H](OC)[C@H](OC)C)N4C(N=C3)=CC(Cl)=N4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (406.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0312 mL	10.1560 mL	20.3120 mL
5 mM	0.4062 mL	2.0312 mL	4.0624 mL
10 mM	0.2031 mL	1.0156 mL	2.0312 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% DMSO 95% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.08 mM); Clear solution
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 4

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (250 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Quancard J, et al. An allosteric MALT1 inhibitor is a molecular corrector rescuing function in an immunodeficient patient. Nat Chem Biol. 2019 Mar;15(3):304-313. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0312 mL	10.1560 mL	20.3120 mL	50.7800 mL
	5 mM	0.4062 mL	2.0312 mL	4.0624 mL	10.1560 mL
	10 mM	0.2031 mL	1.0156 mL	2.0312 mL	5.0780 mL
	15 mM	0.1354 mL	0.6771 mL	1.3541 mL	3.3853 mL
	20 mM	0.1016 mL	0.5078 mL	1.0156 mL	2.5390 mL
	25 mM	0.0812 mL	0.4062 mL	0.8125 mL	2.0312 mL
	30 mM	0.0677 mL	0.3385 mL	0.6771 mL	1.6927 mL
	40 mM	0.0508 mL	0.2539 mL	0.5078 mL	1.2695 mL
	50 mM	0.0406 mL	0.2031 mL	0.4062 mL	1.0156 mL
	60 mM	0.0339 mL	0.1693 mL	0.3385 mL	0.8463 mL
	80 mM	0.0254 mL	0.1269 mL	0.2539 mL	0.6347 mL
	100 mM	0.0203 mL	0.1016 mL	0.2031 mL	0.5078 mL

MLT-748 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MLT-7481832578-30-9MLT748MLT 748MALT1mucosa associated lymphoid tissue lymphoma translocation gene 1Inhibitorinhibitorinhibit

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MLT-748

Customer Review

MLT-748 Related Antibodies

4 Publications Citing Use of MCE MLT-748

Featured Recommendations

•Related Screening Libraries:

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•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

MLT-748 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

MLT-748 Related Classifications

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