2. Metabolic Enzyme/Protease
  3. Oxidative Phosphorylation Mitochondrial Metabolism
  4. Mitochonic acid 5

Mitochonic acid 5  (Synonyms: MA-5)

Cat. No.: HY-111536 Purity: 99.28%
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Mitochonic acid 5 binds mitochondria and ameliorates renal tubular and cardiac myocyte damage. Mitochonic acid 5 modulates mitochondrial ATP synthesis.

For research use only. We do not sell to patients.

Mitochonic acid 5 Chemical Structure

Mitochonic acid 5 Chemical Structure

CAS No. : 1354707-41-7

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10 mM * 1 mL in DMSO
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Customer Review

Based on 3 publication(s) in Google Scholar

Mitochonic acid 5 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Mitochonic acid 5 binds mitochondria and ameliorates renal tubular and cardiac myocyte damage. Mitochonic acid 5 modulates mitochondrial ATP synthesis.

IC50 & Target

Mitochondrial Metabolism[1]
In Vitro

Mitochonic acid 5 (MA-5) modulates mitochondrial ATP synthesis independently of oxidative phosphorylation and the electron transport chain. Mitochondrial dysfunction causes increased oxidative stress and depletion of ATP, which are involved in the etiology of a variety of renal diseases[1]. Mitochonic acid 5 (MA-5), which is derived from the plant growth hormone indole-3-acetic acid, can protect mitochondrial function by regulating energy metabolism and reducing mitochondrial oxidative stress. To observe the protective role of Mitochonic acid 5 in microglia under inflammatory conditions, TNFα is applied. Subsequently, the MTT assay is used to evaluate cell viability. In response to the TNFα treatment, cell viability significantly decreases. However, this effect is dose-dependently inhibited by Mitochonic acid 5 treatment[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mitochonic acid 5 Related Antibodies
In Vivo

Administration of Mitochonic acid 5 (MA-5) to an ischemia-reperfusion injury model and a cisplatin-induced nephropathy model improved renal function. To examine the tissue-protective effect of Mitochonic acid 5, the oral bioavailability is examined. Oral administration of Mitochonic acid 5 increases the plasma concentration in a dose-response manner at the peak time of 1 hour[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

329.30

Formula

C18H13F2NO3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)C(CC(C1=CC=C(F)C=C1F)=O)C2=CNC3=C2C=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (303.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0367 mL 15.1837 mL 30.3674 mL
5 mM 0.6073 mL 3.0367 mL 6.0735 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Cell Assay
[2]

The mouse BV-2 cells used in this study are cultured in L-DMEM supplemented with 10% fetal bovine serum (FBS) at 37°C in an atmosphere with 5% CO2. To induce inflammatory injury, cells are treated with 10 ng/mL TNFα for about 12 h. Mitochonic acid 5 (0-10 μM) is incubated with BV-2 cells for about 12 h with TNFα treatment[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Mice[1]
For evaluation of the blood concentrations of Mitochonic acid 5 (MA-5), Mitochonic acid 5 is orally administered at doses of 25, 50, or 150 mg/kg to C57/BL 6 mice, and blood samples are collected at the designated times. After 1 hour, the mice are euthanized. The blood concentration of Mitochonic acid 5 is determined by LC/MS/MS[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0367 mL 15.1837 mL 30.3674 mL 75.9186 mL
5 mM 0.6073 mL 3.0367 mL 6.0735 mL 15.1837 mL
10 mM 0.3037 mL 1.5184 mL 3.0367 mL 7.5919 mL
15 mM 0.2024 mL 1.0122 mL 2.0245 mL 5.0612 mL
20 mM 0.1518 mL 0.7592 mL 1.5184 mL 3.7959 mL
25 mM 0.1215 mL 0.6073 mL 1.2147 mL 3.0367 mL
30 mM 0.1012 mL 0.5061 mL 1.0122 mL 2.5306 mL
40 mM 0.0759 mL 0.3796 mL 0.7592 mL 1.8980 mL
50 mM 0.0607 mL 0.3037 mL 0.6073 mL 1.5184 mL
60 mM 0.0506 mL 0.2531 mL 0.5061 mL 1.2653 mL
80 mM 0.0380 mL 0.1898 mL 0.3796 mL 0.9490 mL
100 mM 0.0304 mL 0.1518 mL 0.3037 mL 0.7592 mL
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Mitochonic acid 5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mitochonic acid 51354707-41-7MA-5Mitochonic acid5Mitochonic acid-5MA5MA 5Oxidative PhosphorylationMitochondrial MetabolismInhibitorinhibitorinhibit

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